An electrochemical peptide sensor for detection of dengue fever biomarker NS1

Lim, Jong Min; Kim, Ji Hong; Ryu, Myung Yi; Cho, Chae Hwan; Park, Tae Jung; Park, Jong Pil

doi:10.1016/j.aca.2018.04.005

Cited by 53 publications

(44 citation statements)

References 30 publications

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“…For the finest peptide (EHDRMHAYYLTR) shown, K d = 3.9 nM, while the whole electrochemical biosensor provided an LOD of 0.025 ug/mL. The obtained result is far lower than the level of viral proteins in patients' bloodstream (about 50 ug/mL) [53].…”

Section: Voltammetric and Impedimetricmentioning

confidence: 79%

“…For phage-displayed peptides, the apparent dissociation constant is usually determined by the correlation of absorbance or fluorescence with the concentration of the incubated epitope-carrying bacteriophages with the targeted molecule [50][51][52]. Additionally, apparent dissociation constant may be calculated from electrochemical impedance spectroscopy (EIS) by plotting relative resistance change against concentration [53]. In Table 1, we summarize several bacteriophage-based and peptide-based sensors detecting disease markers.…”

Section: M13 Bacteriophage-based and Peptide-based Sensorsmentioning

confidence: 99%

“…These marvellous parameters of the sensor were obtained thanks to a dense receptor layer provided by landscape library and high sensitivity of EIS [58,76]. An analogous scheme was used for detection of the Dengue virus type 2 marker (DENV2 NS1 protein) [53]. A random 12-residues peptide library (Ph.D.-12) was screened and the binding clones were collected and characterized.…”

Section: Voltammetric and Impedimetricmentioning

confidence: 99%

“…This approach was used to develop sensors for detection of troponin I [51,65], myoglobin [69,70], alanine aminotransferase [66] or Norovirus coat protein [67,68]. An analogous scheme was used for detection of the Dengue virus type 2 marker (DENV2 NS1 protein) [53]. A random 12-residues peptide library (Ph.D.-12) was screened and the binding clones were collected and characterized.…”

Section: Voltammetric and Impedimetricmentioning

confidence: 99%

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Phage-Based Sensors in Medicine: A Review

2020

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Bacteriophages are interesting entities on the border of biology and chemistry. In nature, they are bacteria parasites, while, after genetic manipulation, they gain new properties, e.g., selectively binding proteins. Owing to this, they may be applied as recognition elements in biosensors. Combining bacteriophages with different transducers can then result in the development of innovative sensor designs that may revolutionize bioanalytics and improve the quality of medical services. Therefore, here, we review the use of bacteriophages, or peptides from bacteriophages, as new sensing elements for the recognition of biomarkers and the construction of the highly effective diagnostics tools.

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Section: Voltammetric and Impedimetricmentioning

confidence: 79%

Section: M13 Bacteriophage-based and Peptide-based Sensorsmentioning

confidence: 99%

Section: Voltammetric and Impedimetricmentioning

confidence: 99%

Section: Voltammetric and Impedimetricmentioning

confidence: 99%

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Phage-Based Sensors in Medicine: A Review

2020

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“…Presently, many strategies have been developed to detect DENV based on the measurement of specific biomarkers of DENV infection, such as soluble non-structural protein 1 (NS1) [ 9 , 10 , 11 ], DENV envelope protein [ 12 , 13 ], anti-DENV IgM or IgG antibodies [ 14 ] and DENV nucleic acid fragments [ 15 , 16 , 17 , 18 , 19 , 20 , 21 ] in patient sera. However, the immunoreactive kits cannot differentiate the serotypes of DENV.…”

Section: Introductionmentioning

confidence: 99%

A Label-Free Impedimetric Genosensor for the Nucleic Acid Amplification-Free Detection of Extracted RNA of Dengue Virus

Yen

Lai

et al. 2020

Sensors

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Developing rapid and sensitive diagnostic methods for dengue virus (DENV) infection is of prime priority because DENV infection is the most prevalent mosquito-borne viral disease. This work proposes an electrochemical impedance spectroscopy (EIS)-based genosensor for the label-free and nucleic acid amplification-free detection of extracted DENV RNA intended for a sensitive diagnosis of DENV infection. A concentration ratio of 0.04 mM 6-mercaptohexanoic acid (MHA) to 1 mM 6-mercapto-1-hexanol (MCH) was selected to modify thin-film gold electrodes as a link to control the coverage of self-designed probe DNA (pDNA) at a density of 4.5 ± 0.4 × 1011 pDNA/cm2. The pDNA/MHA/MCH-modified genosensors are proven to improve the hybridization efficiency of a synthetic 160-mer target DNA (160mtDNA) with a 140-mer electrode side overhang as compared to other MHA/MCH ratio-modified genosensors. The MHA(0.04 mM)/MCH(1 mM)-modified genosensors also present good hybridization efficiency with the extracted DENV serotype 1 (DENV1) RNA samples, having the same electrode side overhangs with the 160mtDNA, showing a low detection limit of 20 plaque forming units (PFU)/mL, a linear range of 102–105 PFU/mL and good selectivity for DENV1. The pDNA density-controlled method has great promise to construct sensitive genosensors based on the hybridization of extracted DENV nucleic acids.

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Preparation of the Low Molecular Weight Serum Proteome for Mass Spectrometry Analysis

Veenstra

2019

Methods in Molecular Biology

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An electrochemical peptide sensor for detection of dengue fever biomarker NS1

Cited by 53 publications

References 30 publications

Phage-Based Sensors in Medicine: A Review

Phage-Based Sensors in Medicine: A Review

A Label-Free Impedimetric Genosensor for the Nucleic Acid Amplification-Free Detection of Extracted RNA of Dengue Virus

Preparation of the Low Molecular Weight Serum Proteome for Mass Spectrometry Analysis

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