A Label-Free Impedimetric Genosensor for the Nucleic Acid Amplification-Free Detection of Extracted RNA of Dengue Virus

Wu, Ching-Chou; Yen, Hao Yu; Lai, Lu; Perng, Guey Chuen; Lee, Cheng Rei; Wu, Shuenn Jue L.

doi:10.3390/s20133728

Cited by 17 publications

(5 citation statements)

References 41 publications

(72 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In addition, these analytical devices can work with low sample volumes. More importantly, biosensors can be used for the label-free and nucleic acid amplification-free detection of DNA/RNA for the sensitive diagnosis of infection [ 38 ]. Therefore, ultralow LOD, ability for simultaneous detection, selectivity, and portability of biosensors make them a better choice for COVID-19 diagnosis.…”

Section: Resultsmentioning

confidence: 99%

A nanoscale genosensor for early detection of COVID-19 by voltammetric determination of RNA-dependent RNA polymerase (RdRP) sequence of SARS-CoV-2 virus

Farzin¹,

Sadjadi²,

Sheini³

et al. 2021

Microchim Acta

View full text Add to dashboard Cite

Graphical abstract A voltammetric genosensor has been developed for the early diagnosis of COVID-19 by determination of RNA-dependent RNA polymerase (RdRP) sequence as a specific target of novel coronavirus. The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) uses an RdRP for the replication of its genome and the transcription of its genes. Here, the silver ions (Ag + ) in the hexathia-18-crown-6 (HT18C6) were used for the first time as a redox probe. Then, the HT18C6(Ag) incorporated carbon paste electrode (CPE) was further modified with chitosan and PAMAM dendrimer-coated silicon quantum dots (SiQDs@PAMAM) for immobilization of probe sequences (aminated oligonucleotides). The current intensity of differential pulse voltammetry using the redox probe was found to decrease with increasing the concentration of target sequence. Based on such signal-off trend, the proposed genosensor exhibited a good linear response to SARS-CoV-2 RdRP in the concentration range 1.0 pM–8.0 nM with a regression equation I (μA) = − 6.555 log [RdRP sequence] (pM) + 32.676 ( R 2 = 0.995) and a limit of detection (LOD) of 0.3 pM. The standard addition method with different spike concentrations of RdRP sequence in human sputum samples showed a good recovery for real sample analysis (> 95%). Therefore, the developed voltammetric genosensor can be used to determine SARS-CoV-2 RdRP sequence in sputum samples. PAMAM-functionalized SiQDs were used as a versatile electrochemical platform for the SARS-CoV-2 RdRP detection based on a signal off sensing strategy. In this study, for the first time, the silver ions (Ag + ) in the hexathia-18-crown-6 carrier were applied as an electrochemical probe. Supplementary Information The online version contains supplementary material available at 10.1007/s00604-021-04773-6.

show abstract

Section: Resultsmentioning

confidence: 99%

A nanoscale genosensor for early detection of COVID-19 by voltammetric determination of RNA-dependent RNA polymerase (RdRP) sequence of SARS-CoV-2 virus

Farzin¹,

Sadjadi²,

Sheini³

et al. 2021

Microchim Acta

View full text Add to dashboard Cite

show abstract

“…As a receptor, the complement was invented. Because of its high selectivity, this research may be generated as the targeted sequence with other DENV serotypes 1–4 based on electrochemical impedance spectroscopy studies . Furthermore, the 27-mer DNA target of DENV (3′-TTG TCG TAT AAC TGC GAC CCT CTC TGG-5′) could be detected by the 27-mer amine terminated probe (5′-NH2-C6-AAC AGC ATA TTG ACG CTG GGA GAG ACC-3′).…”

Section: Peptide Discovery Through Receptor–flavivirus Interactionmentioning

confidence: 99%

“…Because of its high selectivity, this research may be generated as the targeted sequence with other DENV serotypes 1−4 based on electrochemical impedance spectroscopy studies. 115 Furthermore, the 27-mer DNA target of DENV (3′-TTG TCG TAT AAC TGC GAC CCT CTC TGG-5′) could be detected by the 27-mer amine terminated probe (5′-NH2-C6-AAC AGC ATA TTG ACG CTG GGA GAG ACC-3′). Through the electrical biosensor, the 27-mer terminated amine could selectively detect the complementarity among the mismatched DNA and noncomplementary DNA.…”

Section: Peptide Discovery Through Receptor−flavivirus Interactionmentioning

confidence: 99%

Peptide-Based Flavivirus Biosensors: From Cell Structure to Virological and Serological Detection Methods

Muslihati,

Septiani,

Gumilar

et al. 2024

ACS Biomater. Sci. Eng.

View full text Add to dashboard Cite

In tropical and developing countries, mosquito-borne diseases by flaviviruses pose a serious threat to public health. Early detection is critical for preventing their spread, but conventional methods are time-consuming and require skilled technicians. Biosensors have been developed to address this issue, but cross-reactivity with other flaviviruses remains a challenge. Peptides are essentially biomaterials used in diagnostics that allow virological and serological techniques to identify flavivirus selectively. This biomaterial originated as a small protein consisting of two to 50 amino acid chains. They offer flexibility in chemical modification and can be easily synthesized and applied to living cells in the engineering process. Peptides could potentially be developed as robust, low-cost, sensitive, and selective receptors for detecting flaviviruses. However, modification and selection of the receptor agents are crucial to determine the effectiveness of binding between the targets and the receptors. This paper addresses two potential peptide nucleic acids (PNAs) and affinity peptides that can detect flavivirus from another target-based biosensor as well as the potential peptide behaviors of flaviviruses. The PNAs detect flaviviruses based on the nucleotide base sequence of the target's virological profile via Watson−Crick base pairing, while the affinity peptides sense the epitope or immunological profile of the targets. Recent developments in the functionalization of peptides for flavivirus biosensors are explored in this Review by division into electrochemical, optical, and other detection methods.

show abstract

“…Among emerging technologies available, DNA biosensor, an analytical device incorporating a single-stranded oligonucleotide (probe) linked with a physicochemical transducer, offers an interesting alternative test. In recent years, this technology has been studied widely as a potential novel method for the detection of DNA hybridization in various fields such as the diagnosis of diseases including cancer [ 25 , 26 , 27 ], the detection of infectious agents [ 28 , 29 ], drug screening [ 30 , 31 , 32 ], crops screening [ 33 , 34 ], and forensic applications [ 35 , 36 ].…”

Section: Introductionmentioning

confidence: 99%

Sequence-Specific Electrochemical Genosensor for Rapid Detection of blaOXA-51-like Gene in Acinetobacter baumannii

et al. 2022

View full text Add to dashboard Cite

Acinetobacter baumannii (A. baumannii) are phenotypically indistinguishable from the Acinetobacter calcoaceticus–A. baumannii (ACB) complex members using routine laboratory methods. Early diagnosis plays an important role in controlling A. baumannii infections and this could be assisted by the development of a rapid, yet sensitive diagnostic test. In this study, we developed an enzyme-based electrochemical genosensor for asymmetric PCR (aPCR) amplicon detection of the blaOXA-51-like gene in A. baumannii. A. baumannii blaOXA-51-like gene PCR primers were designed, having the reverse primer modified at the 5′ end with FAM. A blaOXA-51-like gene sequence-specific biotin labelled capture probe was designed and immobilized using a synthetic oligomer (FAM-labelled) deposited on the working electrode of a streptavidin-modified, screen-printed carbon electrode (SPCE). The zot gene was used as an internal control with biotin and FAM labelled as forward and reverse primers, respectively. The blaOXA-51-like gene was amplified using asymmetric PCR (aPCR) to generate single-stranded amplicons that were detected using the designed SPCE. The amperometric current response was detected with a peroxidase-conjugated, anti-fluorescein antibody. The assay was tested using reference and clinical A. baumannii strains and other nosocomial bacteria. The analytical sensitivity of the assay at the genomic level and bacterial cell level was 0.5 pg/mL (1.443 µA) and 103 CFU/mL, respectively. The assay was 100% specific and sensitive for A. baumannii. Based on accelerated stability performance, the developed genosensor was stable for 1.6 years when stored at 4 °C and up to 28 days at >25 °C. The developed electrochemical genosensor is specific and sensitive and could be useful for rapid, accurate diagnosis of A. baumannii infections even in temperate regions.

show abstract

A Label-Free Impedimetric Genosensor for the Nucleic Acid Amplification-Free Detection of Extracted RNA of Dengue Virus

Cited by 17 publications

References 41 publications

A nanoscale genosensor for early detection of COVID-19 by voltammetric determination of RNA-dependent RNA polymerase (RdRP) sequence of SARS-CoV-2 virus

A nanoscale genosensor for early detection of COVID-19 by voltammetric determination of RNA-dependent RNA polymerase (RdRP) sequence of SARS-CoV-2 virus

Peptide-Based Flavivirus Biosensors: From Cell Structure to Virological and Serological Detection Methods

Sequence-Specific Electrochemical Genosensor for Rapid Detection of blaOXA-51-like Gene in Acinetobacter baumannii

Contact Info

Product

Resources

About