An electrochemical nanobiosensor for plasma miRNA-155, based on graphene oxide and gold nanorod, for early detection of breast cancer

Azimzadeh, Mostafa; Rahaie, Mahdi; Nasirizadeh, Navid; Ashtari, Khadijeh; Naderi-Manesh, Hossein

doi:10.1016/j.bios.2015.09.020

Cited by 294 publications

(121 citation statements)

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“…It is worth noting that commonly used miRNA determination techniques such as northern blot and qRT-PCR provide a significantly low detection limit (aM-nM) and excellent selectivity [21]. However, their complex time-consuming procedure along with the need for amplification steps has made them inappropriate for routine clinical screening.…”

Section: Resultsmentioning

confidence: 99%

“…Over the past years, miRNAs have been one of the most attractive targets for the construction of electrochemical nanobiosensors [8,21,28]. There are numerous studies on fabricating electrochemical nanobiosensors for the detection of label-free or labeled miRNA.…”

Section: Introductionmentioning

confidence: 99%

“…There are numerous studies on fabricating electrochemical nanobiosensors for the detection of label-free or labeled miRNA. Although biosensors based on electrochemical impedance spectroscopy (EIS) offered a label-free methodology and reached a low detection limit for miRNA [29], most of the label-free miRNA biosensors are developed using molecular probes such as methylene blue [30], Oracet blue [21], and ferrocene boronic acid [31]. …”

Section: Introductionmentioning

confidence: 99%

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A novel electrochemical nanobiosensor for the ultrasensitive and specific detection of femtomolar-level gastric cancer biomarker miRNA-106a

Daneshpour¹,

Omidfar²,

Ghanbarian³

2016

Beilstein J. Nanotechnol.

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Gastric cancer (GC) is the second leading cause of cancer-related deaths all over the world. miR-106a is a circulatory oncogenic microRNA (miRNA), which overexpresses in various malignancies, especially in GC. In this study, an ultrasensitive electrochemical nanobiosensor was developed for the detection of miR-106a using a double-specific probe methodology and a gold–magnetic nanocomposite as tracing tag. The successful modification of the electrode and hybridization with the target miRNA were confirmed by electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) methods. Differential pulse voltammetry (DPV) was used for quantitative evaluation of miR-106a via recording the reduction peak current of gold nanoparticles. The electrochemical signal had a linear relationship with the concentration of the target miRNA ranging from 1 × 10−3 pM to 1 × 103 pM, and the detection limit was 3 × 10−4 pM. The proposed miRNA-nanobiosensor showed remarkable selectivity, high specificity, agreeable storage stability, and great performance in real sample investigation with no pretreatment or amplification. Consequently, our biosensing strategy offers such a promising application to be used for clinical early detection of GC and additionally the screen of any miRNA sequence.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A novel electrochemical nanobiosensor for the ultrasensitive and specific detection of femtomolar-level gastric cancer biomarker miRNA-106a

Daneshpour¹,

Omidfar²,

Ghanbarian³

2016

Beilstein J. Nanotechnol.

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“…This induces cytotoxic effects that lead to an irreversible photodamage of cancer cells [71][72][73][74]77]. In this regard, carbon nanomaterials have been used successfully to cause thermal ablation of solid tumors in breast cancer studies [16,68,[74][75][76][79][80][81][82]. Ogbodu et al [74] reported for the first time the synthesis, photophysical properties, and PDT activity of ZnMCPPc-spermine-SWCNT (zinc monocarboxy phenoxy phthalocyanine upon conjugation to spermidine, as a targeting molecule, and then adsorbed onto SWCNT) on breast cancer cells (MCF-7).…”

Section: Photodynamicmentioning

confidence: 99%

“…Biosensors are able to identify tumor markers at such a low concentration (traces) where other techniques, such as image studies, may fail. Therefore, the numbers of studies towards the use of carbon nanomaterials as biosensors have increased [16,[82][83][84].…”

Section: Biosensorsmentioning

confidence: 99%

Carbon Nanomaterials for Breast Cancer Treatment

Casais-Molina

Cab

Canto

et al. 2018

Journal of Nanomaterials

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Currently, breast cancer is considered as a health problem worldwide. Furthermore, current treatments neither are capable of stopping its propagation and/or recurrence nor are specific for cancer cells. Therefore, side effects on healthy tissues and cells are common. An increase in the efficiency of treatments, along with a reduction in their toxicity, is desirable to improve the life quality of patients affected by breast cancer. Nanotechnology offers new alternatives for the design and synthesis of nanomaterials that can be used in the identification, diagnosis, and treatment of cancer and has now become a very promising tool for its use against this disease. Among the wide variety of nanomaterials, the scientific community is particularly interested in carbon nanomaterials (fullerenes, nanotubes, and graphene) due to their physical properties, versatile chemical functionalization, and biocompatibility. Recent scientific evidence shows the potential uses of carbon nanomaterials as therapeutic agents, systems for selective and controlled drug release, and contrast agents for diagnosing and locating tumors. This generates new possibilities for the development of innovative systems to treat breast cancer and can be used to detect this disease at much earlier stages. Thus, applications of carbon nanomaterials in breast cancer treatment are discussed in this article.

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