An Electrochemical Molecularly Imprinted Polymer Sensor for Rapid β-Lactoglobulin Detection

Abstract

Facile detection of β-lactoglobulin is extraordinarily important for the management of the allergenic safety of cow’s milk and its dairy products. A sensitive electrochemical sensor based on a molecularly imprinted polymer-modified carbon electrode for the detection of β-lactoglobulin was successfully synthesized. This molecularly imprinted polymer was prepared using a hydrothermal method with choline chloride as a functional monomer, β-lactoglobulin as template molecule and ethylene glycol dimethacrylate as crosslinking agent. Then, the molecularly imprinted polymer was immobilized on polyethyleneimine (PEI)-reduced graphene oxide (rGO)-gold nanoclusters (Au-NCs) to improve the sensor’s selectivity for β-lactoglobulin. Under optimal experimental conditions, the designed sensor showed a good response to β-lactoglobulin, with a linear detection range between 10−9 and 10−4 mg/mL, and a detection limit of 10−9 mg/mL (S/N = 3). The developed electrochemical sensor showed a high correlation in the detection of β-lactoglobulin in four different milk samples from the market, indicating that the sensor can be used with actual sample.

Recent Advances in Electrochemical Sensing Strategies for Food Allergen Detection

“…An electrochemical sensor based on MIP was developed for β-LB detection using choline chloride as a functional monomer, β-LB as the template molecule, ethylene glycol dimethacrylate (EDMA) as the cross-linking agent, and benzoyl-NN-dimethylaniline (BPO-DMA) as the polymerization initiator [ 178 ]. Next, the polymer was immobilized onto an SPCE modified with a nanocomposite including polyethyleneimine (PEI)-reduced graphene oxide (rGO) and AuNCs, as illustrated in Figure 17 .…”

Recent Advances of Nanomaterials-Based Molecularly Imprinted Electrochemical Sensors

“…Au-S and intermolecular forces between PDA and functional monomers guaranteed the stable immobilization of MIP film. Wang et al [ 84 ] successfully synthesized a polyethyleneimine (PEI)-rGO-gold nanoclusters (Au-NCs)@MIP sensor. Both rGO and AuNPs in nanocomposite could diminish the electrode surface resistance, making recognition of β-lactoglobulin (β-LG) faster and more accurate.…”

Coaxial dual-path electrochemical biosensing and logic strategy-based detection of lung cancer-derived exosomal PD-L1

“…Compared with traditional detection methods such as fluorescence, the use of electrochemical biosensors has gained widespread attention in marker detection due to their high sensitivity and ease of operation, 6,7 but they still face challenges in the detection of low abundance ExoPD-L1. Advances in nanotechnology have enabled nanomaterials to show outstanding advantages in highly sensitive detection of electrochemical biosensors.…”