An electrochemical investigation into the effects of local and systemic administrations of sodium nitroprusside in brain extracellular fluid of mice

Reid, Caroline H.; Finnerty, Niall J.

doi:10.1016/j.bioelechem.2019.107441

Cited by 5 publications

(5 citation statements)

References 55 publications

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“…While both SNP and SNAP can spontaneously release 1 mol of • NO per mole of the parent compound, this depends on the composition of the dissolution medium. For SNP, this requires it to be dissolved in a medium with a specific pH in the presence of a particular reducing agent such as Na 2 SO 4 and ascorbic acid, which was not the case in the current study. This can account for the weaker intensity of the band at 1740 cm –1 in the case of HA/SNP than that of HA/SNAP.…”

Section: Resultsmentioning

confidence: 95%

Interactions between Nitric Oxide and Hyaluronan Implicate the Migration of Breast Cancer Cells

et al. 2022

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Nitric oxide ( • NO) is one of the prominent free radicals, playing a pivotal role in breast cancer progression. Hyaluronic acid (HA) plays an essential role in neutralizing free radicals in tumor tissues. However, its interactions with nitric oxide have not been thoroughly investigated. Hence, this study attempts to understand the mechanism of these interactions and the different effects on the intracellular • NO levels and migration of breast cancer cells. The affinity of HA to scavenge • NO was investigated alongside the accompanying changes in specific physico-chemical properties and the further effects on the • NO-induced attachment and migration of the breast cancer cell lines, MDA-MB-231 and HCC1806. The reaction of the nitrogen dioxide radical, formed via • NO/O 2 interactions, with HA initiated a series of oxidative reactions, which, in the presence of • NO, induce the fragmentation of the polymeric chains. Furthermore, these interactions were found to hinder the NO-induced migration of cancer cells. However, the NO-induced HA modification/fragmentation was inhibited in the presence of hemin, a NO-scavenging compound. Collectively, these results help toward understanding the involvement of HA in the • NO-induced cell migration and suggest the possible modification of HA, used as one of the main materials in different biomedical applications.

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Section: Resultsmentioning

confidence: 95%

Interactions between Nitric Oxide and Hyaluronan Implicate the Migration of Breast Cancer Cells

et al. 2022

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“…Typically, in an in vivo setting, a three-electrode system is used: (i) the working electrode (which is the biosensor), (ii) the reference electrode, and (iii) a counter electrode [ 67 , 69 , 70 ]. Specifically, to monitor extracellular concentrations in the brain tissue, the working electrode(s) is (are) implanted in the brain region of interest ( Figure 2 c), while the reference electrode can be implanted in the cortex and the counter electrode can be attached to an anchor screw placed in the skull [ 71 ].…”

Section: Technical and Historical Overviewmentioning

confidence: 99%

“…The signal can be continuously monitored from the moment just after implantation, although it is important to wait for the baseline to stabilize before initiating an experiment. For example, some groups start measuring minutes after implantation [ 53 , 59 ], while others wait a few hours [ 71 ], although the latter strongly depends on the type of electrode that is used and the experimental setup (awake vs. under anesthesia) [ 85 ]. Hence, this equilibration period is based on the sensor rather than on brain homeostasis and the integrity of the BBB.…”

Section: Strengths and Limitationsmentioning

confidence: 99%

“…These results were confirmed in vivo up to 8 days after implantation [ 96 ]. Generally, chronic in vivo cerebral biosensor experiments are performed for a maximum of 2–3 weeks, although this strongly depends on the sensor used [ 71 , 96 , 97 , 98 ].…”

Section: Strengths and Limitationsmentioning

confidence: 99%

“…There is no consensus on the timeframe to perform cerebral biosensor experiments. However, the signal can be continuously monitored from the moment upon baseline stabilization after implantation, with a maximum duration of 2–3 weeks, although the latter strongly depends on the type of sensors that are used [ 71 , 98 ].…”

Section: Strengths and Limitationsmentioning

confidence: 99%

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Current Approaches to Monitor Macromolecules Directly from the Cerebral Interstitial Fluid

et al. 2022

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Gaining insights into the pharmacokinetic and pharmacodynamic properties of lead compounds is crucial during drug development processes. When it comes to the treatment of brain diseases, collecting information at the site of action is challenging. There are only a few techniques available that allow for the direct sampling from the cerebral interstitial space. This review concerns the applicability of microdialysis and other approaches, such as cerebral open flow microperfusion and electrochemical biosensors, to monitor macromolecules (neuropeptides, proteins, …) in the brain. Microdialysis and cerebral open flow microperfusion can also be used to locally apply molecules at the same time at the site of sampling. Innovations in the field are discussed, together with the pitfalls. Moreover, the ‘nuts and bolts’ of the techniques and the current research gaps are addressed. The implementation of these techniques could help to improve drug development of brain-targeted drugs.

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A self-powered controllable microneedle drug delivery system for rapid blood pressure reduction

Chen,

Lai,

et al. 2024

Nano Energy

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An electrochemical investigation into the effects of local and systemic administrations of sodium nitroprusside in brain extracellular fluid of mice

Cited by 5 publications

References 55 publications

Interactions between Nitric Oxide and Hyaluronan Implicate the Migration of Breast Cancer Cells

Interactions between Nitric Oxide and Hyaluronan Implicate the Migration of Breast Cancer Cells

Current Approaches to Monitor Macromolecules Directly from the Cerebral Interstitial Fluid

A self-powered controllable microneedle drug delivery system for rapid blood pressure reduction

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