An electrochemical immunosensor for the detection of Glypican-3 based on enzymatic ferrocene-tyramine deposition reaction

Lu, Wenxi; Xie, Xixiang; Lan, Xianli; Wu, Pan; Peng, Hongmei; Zhong, Liping; Liu, Xiyu; Deng, Zhiming; Tan, Zhenkai; Wu, Anxin; Shi, Liang; Huang, Yong

doi:10.1016/j.bios.2023.115081

Cited by 10 publications

(6 citation statements)

References 29 publications

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“…W. Lu et al used the magnetic bead-GPC3 antibody-GPC3 antigen-HRP coupling as a catalytic inducer to induce the deposition of large amounts of ferrocene-tyramine (Fc-Tyr) on the surface of the SPCE/Au NP/3-mercaptopropionic acid (MPA)/Tyr electrode, generating enhanced ferrocene redox currents so that the analyte concentration could be quantified by recording the current signal of the Fc molecules, and the concentration range is 0.1–1 ng ml −1 . 66 (Fig. 7b) P. Chikhaliwala et al used magnetite nanoparticles (Fe 3 O 4 NPs) functionalized with first-generation poly-amidoamine dendrimers (G1; PAMAM) as an electrochemical biosensing platform for early diagnosis of HCC which can simultaneously measure GPC3 from a single sample.…”

Section: Non-optical Inspection Methodsmentioning

confidence: 99%

Current methods for the detection of glypican-3

Xiao,

Huang,

Lin

et al. 2024

Anal. Methods

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Section: Non-optical Inspection Methodsmentioning

confidence: 99%

Current methods for the detection of glypican-3

Xiao,

Huang,

Lin

et al. 2024

Anal. Methods

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“…As shown in Table S1, the dynamic range of this TSA-amplified PGM immunoassay with 5 orders of magnitude is also better than that of PGM-based immunoassays. Moreover, compared with the reported TSA-based electrochemical immunoassay, 27 chemiluminescence immunoassay, 28 surface-enhanced Raman scattering immunoassay, 29 and electrochemiluminescence immunoassay, 30 the proposed TSA-PGM immunoassay possesses unique advantages that are simple, cost-effective, and easy to popularize and is not limited by the sophisticated equipment and technologies and special laboratories. Therefore, the TSA-PGM immunoassay provides a simple and efficient strategy for the detection of a low level of proteins by integration of the TSA reaction and PGM readout.…”

Section: ■ Introductionmentioning

confidence: 99%

Tyramine-Invertase Bioconjugate-Amplified Personal Glucose Meter Signaling for Ultrasensitive Immunoassay

Dai,

Zhang,

et al. 2024

Anal. Chem.

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Highly sensitive and facile detection of low levels of protein markers is of great significance for the early diagnosis and efficacy monitoring of diseases. Herein, aided by an efficient tyramine-signal amplification (TSA) mechanism, we wish to report a simple but ultrasensitive immunoassay with signal readout on a portable personal glucose meter (PGM). In this study, the bioconjugates of tyramine and invertase (Tyr-inv), which act as the critical bridge to convert and amplify the protein concentration information into glucose, are prepared following a click chemistry reaction. Then, in the presence of a target protein, the sandwich immunoreaction between the immobilized capture antibody, the target protein, and the horseradish peroxidase (HRP)-conjugated detection antibody is specifically performed in a 96-well microplate. Subsequently, the specifically loaded HRP-conjugated detection antibodies will catalyze the amplified deposition of a large number of Tyr-inv molecules onto adjacent proteins through highly efficient TSA. Then, the deposited invertase, whose dosage can faithfully reflect the original concentration of the target protein, can efficiently convert sucrose to glucose. The amount of finally produced glucose is simply quantified by the PGM, realizing the highly sensitive detection of trace protein markers such as the carcinoembryonic antigen and alpha fetoprotein antigen at the fg/mL level. This method is simple, cost-effective, and ultrasensitive without the requirement of sophisticated instruments or specialized laboratory equipment, which may provide a universal and promising technology for highly sensitive immunoassay for in vitro diagnosis of diseases.

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“…15 At the same time, electrochemistry-based POCT devices have gained great attention because of their several advantages, including high sensitivity, low cost, rapid response and easy operation. 16,17 For example, Chen et al reported an integrated flexible electrode for the rapid detection of cancer-related RNA in human urine samples with excellent sensitivity and stability. 18…”

Section: Introductionmentioning

confidence: 99%

“…15 At the same time, electrochemistrybased POCT devices have gained great attention because of their several advantages, including high sensitivity, low cost, rapid response and easy operation. 16,17 For example, Chen et al reported an integrated flexible electrode for the rapid detection of cancer-related RNA in human urine samples with excellent sensitivity and stability. 18 To fabricate microelectrodes for integrated electrochemical immunosensors, screen printing technology is considered an ideal technique because of its unique advantages such as simplicity, speed, efficiency and mass production.…”

Section: Introductionmentioning

confidence: 99%