An electrochemical immunosensing system on patterned electrodes for immunoglobulin E detection

Oh, Seung Jun; Ahn, Jun Ki; Park, Hun; Song, Yesol; Kwon, Seong Jung; Shin, Hang-Beum

doi:10.1039/c9ay01257e

Cited by 8 publications

(5 citation statements)

References 21 publications

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“…In this research, we introduced the electron transfer mediator, Fc, as a functionality in the APTES SAMs-modified ITO electrode. In previous studies [33][34][35], the Fc-modified SAMs were constructed on the Au electrode by a complicated method: for example, using a specially designed Fc-derivative, such as an Fc-tethered dendrimer or Fc-functionalized thiols. Therefore, less reproducible mixed SAMs using multiple thiols and additional immobilization steps using a crosslinker were required for the introduction of the Fc moiety in the electrochemical sensing layer.…”

Section: Preparation Of Fc-modified Sams On the Ito Electrodementioning

confidence: 99%

Electrochemical Immunosensor for Human IgE Using Ferrocene Self-Assembled Monolayers Modified ITO Electrode

et al. 2020

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The immunoglobulin E (IgE) level in serum is an important factor in the examination of allergy. Ferrocene (Fc)-modified self-assembled monolayers (SAMs) were placed on an indium tin oxide (ITO) electrode as a sensing layer for the detection of human IgE. The Fc moiety in the SAMs facilitated the electron transfer through the organic SAMs layer and electrocatalytic signal amplification. The electrochemical measurement was accomplished after the sandwich type immobilization of the receptor antibody, target human IgE, and enzyme conjugated secondary antibody. The enzyme product, p-aminophenol, was quantitatively analyzed by redox cycling via Fc. In addition, the electrochemical impedance spectroscopy (EIS) was investigated for the detection of IgE. The limit of detection (LOD), limit of quantification (LOQ), and dynamic range of the electrochemical sensor were 3 IU/mL, 10 IU/mL, and from 10 IU/mL to 100 IU/mL, respectively.

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Section: Preparation Of Fc-modified Sams On the Ito Electrodementioning

confidence: 99%

Electrochemical Immunosensor for Human IgE Using Ferrocene Self-Assembled Monolayers Modified ITO Electrode

et al. 2020

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“…The Nernst equation defines how the concentration of the species in solution near the working electrode changes over time [52]. Oh et al applied CV to an immunosensing platform for detecting IgE in multiple samples simultaneously [53]. A gold electrode surface was treated with a mixed self-assembled monolayer (SAM) containing 11-mercapto-1-undecanol (MUO), 12-mercaptododecanoic acid (MDA) and 11-(mercaptoundecyl)ferrocene (MUF).…”

Section: Electrochemical Biosensorsmentioning

confidence: 99%

Biosensors for Detecting Lymphocytes and Immunoglobulins

et al. 2020

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Lymphocytes (B, T and natural killer cells) and immunoglobulins are essential for the adaptive immune response against external pathogens. Flow cytometry and enzyme-linked immunosorbent (ELISA) kits are the gold standards to detect immunoglobulins, B cells and T cells, whereas the impedance measurement is the most used technique for natural killer cells. For point-of-care, fast and low-cost devices, biosensors could be suitable for the reliable, stable and reproducible detection of immunoglobulins and lymphocytes. In the literature, such biosensors are commonly fabricated using antibodies, aptamers, proteins and nanomaterials, whereas electrochemical, optical and piezoelectric techniques are used for detection. This review describes how these measurement techniques and transducers can be used to fabricate biosensors for detecting lymphocytes and the total content of immunoglobulins. The various methods and configurations are reported, along with the advantages and current limitations.

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“…Driven by this conviction, this work reports the first electroanalytical bioplatform reported so far for the single and simultaneous determination of OVA-specific IgE and IgG4. It is important to note that although some electrochemical biosensors for IgEs have been previously reported, 1,30-33 some of them require a long preparation time 32,33 or have limited sensitivity 31 and none of them dealt with the determination of specific IgE against food allergens. In addition, to our knowledge, no electrochemical bioplatform has been reported so far for the determination of IgG4 or targeting the simultaneous determination of different Igs related to allergic processes.…”

Section: Introductionmentioning

confidence: 99%

Rapid diagnosis of egg allergy by targeting ovalbumin specific IgE and IgG4 in serum on a disposable electrochemical immunoplatform

et al. 2022

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An electrochemical immunosensing system on patterned electrodes for immunoglobulin E detection

Abstract: We present an electrochemical biosensing platform which enables immunoglobulin E (IgE) detection from eight independent biological samples. The performance of the electrochemical immunosensor was comparable to the conventional optical assay.

Cited by 8 publications

References 21 publications

Electrochemical Immunosensor for Human IgE Using Ferrocene Self-Assembled Monolayers Modified ITO Electrode

Electrochemical Immunosensor for Human IgE Using Ferrocene Self-Assembled Monolayers Modified ITO Electrode

Biosensors for Detecting Lymphocytes and Immunoglobulins

Rapid diagnosis of egg allergy by targeting ovalbumin specific IgE and IgG4 in serum on a disposable electrochemical immunoplatform

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