An Electrochemical Immunoassay for HER2 Detection

Al-Khafaji, Q. A. M.; Harris, Mark; Tombelli, Sara; Laschi, Serena; Turner, Anthony P. F.; Mascini, Marco; Marrazza, Giovanna

doi:10.1002/elan.201100501

Cited by 77 publications

(51 citation statements)

References 21 publications

(33 reference statements)

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“…In comparison with the published electrochemical immunoassay for serum HER2 ECD analysis [15], the analysis time of the developed immunosensor in this work was slightly longer (720 min). However, in the previous study the linear calibration curve was established only between 0 and 15 ng/mL (n¼4), the LOD was 6 ng/mL and the reproducibility for spiked serum samples in terms of CV was about 10%.…”

Section: Serum Analysismentioning

confidence: 82%

“…To date two electrochemical immunosensing methods for the analysis of HER2 have been published [14,15]. Mucelli et al [14] used nanoelectrode ensembles as the transducer and a sandwich immunoassay for the detection of HER2 in diluted cell lysates and tumor lysates.…”

Section: Introductionmentioning

confidence: 99%

“…The lowest concentration they analyzed was 40 ng/ mL. Al-Khafaji et al [15] applied antibody-functionalized magnetic beads in combination with a screen-printed carbon electrode. The detection limit of the used sandwich immunoassay was 6 ng/mL.…”

Section: Introductionmentioning

confidence: 99%

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Electrochemical immunosensor for the analysis of the breast cancer biomarker HER2 ECD

Marques

Subramanian

Nouws

et al. 2014

Talanta

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a b s t r a c tHuman epidermal growth factor receptor 2 (HER2) is a breast cancer biomarker that plays a major role in promoting breast cancer cell proliferation and malignant growth. The extracellular domain (ECD) of HER2 can be shed into the blood stream and its concentration is measurable in the serum fraction of blood. In this work an electrochemical immunosensor for the analysis of HER2 ECD in human serum samples was developed. To achieve this goal a screen-printed carbon electrode, modified with gold nanoparticles, was used as transducer surface. A sandwich immunoassay, using two monoclonal antibodies, was employed and the detection of the antibody-antigen interaction was performed through the analysis of an enzymatic reaction product by linear sweep voltammetry. Using the optimized experimental conditions the calibration curve (i p vs. log[HER2 ECD]) was established between 15 and 100 ng/mL and a limit of detection (LOD) of 4.4 ng/mL was achieved. These results indicate that the developed immunosensor could be a promising tool in breast cancer diagnostics, patient follow-up and monitoring of metastatic breast cancer since it allows quantification in a useful concentration range and has an LOD below the established cut-off value (15 ng/mL).

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Section: Serum Analysismentioning

confidence: 82%

Section: Introductionmentioning

confidence: 99%

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Electrochemical immunosensor for the analysis of the breast cancer biomarker HER2 ECD

Marques

Subramanian

Nouws

et al. 2014

Talanta

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“…[1][2][3] Traditional immunoassay methods have included the radioimmunoassay, enzyme-linked immunosorbent assay, and electrochemiluminescence immunoassay. In spite of their good sensitivity, the radioimmunoassay significantly increases the radioactivity hazard for laboratory workers and the enzyme-linked immunosorbent assay is tedious and time-consuming.…”

Section: Introductionmentioning

confidence: 99%

Sensitive electrochemical immunosensor based on three-dimensional nanostructure gold electrode

Zhong¹,

Lan²,

Zhang³

et al. 2015

IJN

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A sensitive electrochemical immunosensor was developed for detection of alpha-fetoprotein (AFP) based on a three-dimensional nanostructure gold electrode using a facile, rapid, “green” square-wave oxidation-reduction cycle technique. The resulting three-dimensional gold nanocomposites were characterized by scanning electron microscopy and cyclic voltammetry. A “sandwich-type” detection strategy using an electrochemical immunosensor was employed. Under optimal conditions, a good linear relationship between the current response signal and the AFP concentrations was observed in the range of 10–50 ng/mL with a detection limit of 3 pg/mL. This new immunosensor showed a fast amperometric response and high sensitivity and selectivity. It was successfully used to determine AFP in a human serum sample with a relative standard deviation of <5% (n=5). The proposed immunosensor represents a significant step toward practical application in clinical diagnosis and monitoring of prognosis.

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“…The most common types of immunosensors are found in electrochemical [7,[14][15][16][17][18][19][20][21], optical [22,23], and gravimetric [24] sensors. It is well known that electrochemical detection methods have been able to sensitively and quickly detect biomolecule targets with high selectivity [25][26][27].…”

Section: Introductionmentioning

confidence: 99%

Design of an Affibody-Based Recognition Strategy for Human Epidermal Growth Factor Receptor 2 (HER2) Detection by Electrochemical Biosensors

2016

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Abstract:In this study, we have designed and realized three simple electrochemical bioassays for the detection of the human epidermal growth factor receptor 2 (HER2) cancer biomarker using magnetic beads coupling screen-printed arrays. The different approaches were based on a sandwich format in which affibody (Af) or antibody (Ab) molecules were coupled respectively to streptavidin or protein A-modified magnetic beads. The bioreceptor-modified beads were used to capture the HER2 protein from the sample and sandwich assay was performed by adding the labeled secondary affibody or the antibody. An enzyme-amplified detection scheme based on the coupling of secondary biotinylated bioreceptor with streptavidin-alkaline phosphatase enzyme conjugate was then applied. The enzyme catalyzed the hydrolysis of the electro-inactive 1-naphthyl-phosphate to the electro-active 1-naphthol, which was detected by means of differential pulse voltammetry (DPV). Each developed assay has been studied and optimized. Furthermore, a thorough comparison of the analytical performances of developed assays was performed. Finally, preliminary experiments using serum samples spiked with HER2 protein were also carried out.

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An Electrochemical Immunoassay for HER2 Detection

Cited by 77 publications

References 21 publications

Electrochemical immunosensor for the analysis of the breast cancer biomarker HER2 ECD

Electrochemical immunosensor for the analysis of the breast cancer biomarker HER2 ECD

Sensitive electrochemical immunosensor based on three-dimensional nanostructure gold electrode

Design of an Affibody-Based Recognition Strategy for Human Epidermal Growth Factor Receptor 2 (HER2) Detection by Electrochemical Biosensors

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