Sensitive electrochemical immunosensor based on three-dimensional nanostructure gold electrode

Zhong, Guang‐Xian; Lan, Ruilong; Zhang, Wenxin; Fu, Fei-Huan; Sun, Yiming; Peng, Hua-Ping; Chen, Tianbin; Cai, Yaqi; Liu, Ailin; Lin, Jianhua; Lin, Xinhua

doi:10.2147/ijn.s76200

Cited by 10 publications

(6 citation statements)

References 38 publications

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“…Electrochemical biosensors have shown potential applications in the detection of phosphorylated proteins/ peptides because of their high sensitivity and specificity. [55][56][57][58][59] Usually, the resulting phosphorylated products could be recognized by the elements such as antiphosphorylated peptide antibodies, 19 metal ions, 20 metal complexes, [21][22][23] and nanoparticles. [24][25][26] Moreover, the use of ATP analogs as the cosubstrates (eg, ferrocene [Fc]-ATP, biotin-ATP, and adenosine 5′-[γ-thio] triphosphate [ATP-S]) can also facilitate the development of various novel electrochemical kinase biosensors.…”

mentioning

confidence: 99%

Gold nanoparticles-based electrochemical method for the detection of protein kinase with a peptide-like inhibitor as the bioreceptor

Sun¹,

Chang²,

Zhou³

et al. 2017

IJN

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This article presents a general method for the detection of protein kinase with a peptide-like kinase inhibitor as the bioreceptor, and it was done by converting gold nanoparticles (AuNPs)-based colorimetric assay into sensitive electrochemical analysis. In the colorimetric assay, the kinase-specific aptameric peptide triggered the aggregation of AuNPs in solution. However, the specific binding of peptide to the target protein (kinase) inhibited its ability to trigger the assembly of AuNPs. In the electrochemical analysis, peptides immobilized on a gold electrode and presented as solution triggered together the in situ formation of AuNPs-based network architecture on the electrode surface. Nevertheless, the formation of peptide–kinase complex on the electrode surface made the peptide-triggered AuNPs assembly difficult. Electrochemical impedance spectroscopy was used to measure the change in surface property in the binding events. When a ferrocene-labeled peptide (Fc-peptide) was used in this design, the network of AuNPs/Fc-peptide produced a good voltammetric signal. The competitive assay allowed for the detection of protein kinase A with a detection limit of 20 mU/mL. This work should be valuable for designing novel optical or electronic biosensors and likely lead to many detection applications.

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mentioning

confidence: 99%

Gold nanoparticles-based electrochemical method for the detection of protein kinase with a peptide-like inhibitor as the bioreceptor

Sun¹,

Chang²,

Zhou³

et al. 2017

IJN

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“…Ab 2 /AuNPs/GCE Pd/APTES-M-CeO 2 -GO-Ab 2 Amp 0.0001-50 3.3×10 -6 (Wei et al, 2016) Ab 1 /GO-MB-AuNPs/GCE AuC-HRP-Ab 2 DPV 0. 005-20 1.5×10 -3 (Shen et al, 2020) Ab 1 /AuNPs-GO/GCE P(VT-co-HEMA)-g-GO/Ab 2 SWV 0.025 -50 1.8×10 -2 (Zhao et al, 2020) Ab 1 /3D AuE HRP/Ab 2 Amp 0.005-50 3.0×10 -3 (Zhong et al, 2015) Ab 1 /rGO-TEPA-Thi-AuNPs/SPCE CMK-3@AuPtNPs-Ab 2 Amp 0.005-100 2.2×10…”

Section: Platformmentioning

confidence: 99%

A sandwich-like configuration with a signal amplification strategy using a methylene blue/aptamer complex on a heterojunction 2D MoSe2/2D WSe2 electrode: Toward a portable and sensitive electrochemical alpha-fetoprotein immunoassay

Chanarsa

Jakmunee

Ounnunkad

2022

Front. Cell. Infect. Microbiol.

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Liver cancer is one of the most common global health problems that features a high mortality rate. Alpha-fetoprotein (AFP) is a potential liver cancer biomarker for the diagnosis of liver cancer. The quantitative detection of AFP at an ultratrace level has important medical significance. Using the reaction of the antibody–antigen pair in an immunosensor enables the sensitive and selective AFP assay. Finding a strategy in signal generation and amplification is challenging to fabricate new sensitive electrochemical immunosensors for AFP detection. This study demonstrates the construction of a simple, reliable, and label-free immunosensor for the detection of AFP on a smart phone. Exfoliated two-dimensional (2D) molybdenum diselenide (MoSe2) and 2D tungsten diselenide (WSe2) were employed to modify the disposable screen-printed carbon electrode (SPCE) to use as the electrochemical platform, which is affixed to a small potentiostat connected to a smart phone. The modified electrode offers antibody immobilization and allows detection of AFP via an immunocomplex forming a sandwich-like configuration with the AFP-corresponding aptamer. A heterojunction 2D MoSe2/2D WSe2 composite improves the SPCE’s reactivity and provides a large surface area and good adsorption capacity for the immobilizing antibodies. The signal generation for the immunosensor is from the electrochemical response of methylene blue (MB) intercalating into the aptamer bound on the electrode. The response for the proposed sandwich-like immunosensor is proportional to the AFP concentration (1.0–50,000 pg ml-1). The biosensor has potential for the development of a simple and robust point-of-care diagnostic platform for the clinical diagnosis of liver cancer, achieving a low limit of detection (0.85 pg ml-1), high sensitivity, high selectivity, good stability, and excellent reproducibility.

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“…Glutaraldehyde (GA), 1‐pyrenebutanoic acid succinimidyl ester (PBSE), phthaloyl chloride and iminithiolane can also be used. Many immunosensors were described in which carbodiimide chemistry was employed for antibody immobilization , , . In general, in this process the primary amine groups of the antibody are covalently bound to the carboxylic acid group present on the sensor surface, through reactive succinimide esters by amine coupling through EDC/NHS chemistry.…”

Section: Antibody Immobilizationmentioning

confidence: 99%

“… and Zhong et al. used similar methodologies based on HRP as label and 3,3′,5,5′ tetramethylbenzidine (TMB) as co‐substrate. The authors combined TMB and H 2 O 2 to apply a less positive potential and thus avoid some interferences.…”

Section: Assay Formats (Immunochemical Interactions)mentioning

confidence: 99%

Electrochemical Biosensing in Cancer Diagnostics and Follow‐up

2018

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In cancer, screening and early detection are critical for the success of the patient's treatment and to increase the survival rate. The development of analytical tools for non‐invasive detection, through the analysis of cancer biomarkers, is imperative for disease diagnosis, treatment and follow‐up. Tumour biomarkers refer to substances or processes that, in clinical settings, are indicative of the presence of cancer in the body. These biomarkers can be detected using biosensors, that, because of their fast, accurate and point of care applicability, are prominent alternatives to the traditional methods. Moreover, the constant innovations in the biosensing field improve the determination of normal and/or elevated levels of tumour biomarkers in patients’ biological fluids (such as serum, plasma, whole blood, urine, etc.). Although several biomarkers (DNA, RNA, proteins, cells) are known, the detection of proteins and circulating tumour cells (CTCs) are the most commonly reported due to their approval as tumour biomarkers by the specialized entities and commonly accepted for diagnosis by medical and clinical teams. Therefore, electrochemical immunosensors and cytosensors are vastly described in this review, because of their fast, simple and accurate detection, the low sample volumes required, and the excellent limits of detection obtained. The biosensing strategies reported for the six most commonly diagnosed cancers (lung, breast, colorectal, prostate, liver and stomach) are summarized and the distinct phases of the sensors’ constructions (surface modification, antibody immobilization, immunochemical interactions, detection approach) and applications are discussed.

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Sensitive electrochemical immunosensor based on three-dimensional nanostructure gold electrode

Cited by 10 publications

References 38 publications

Gold nanoparticles-based electrochemical method for the detection of protein kinase with a peptide-like inhibitor as the bioreceptor

Gold nanoparticles-based electrochemical method for the detection of protein kinase with a peptide-like inhibitor as the bioreceptor

A sandwich-like configuration with a signal amplification strategy using a methylene blue/aptamer complex on a heterojunction 2D MoSe2/2D WSe2 electrode: Toward a portable and sensitive electrochemical alpha-fetoprotein immunoassay

Electrochemical Biosensing in Cancer Diagnostics and Follow‐up

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