An Electrochemical Hydrogen Peroxide Biosensor Based on Polydopamine-Entrapped G-Quadruplex-Hemin DNAzyme

Gao, Ai; Wang, Yu-Ru; He, Xiwen; Yin, Xue‐Bo

doi:10.1016/s1872-2040(11)60575-6

Cited by 16 publications

(8 citation statements)

References 18 publications

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“…From a biosensor perspective an interesting feature of PDA is the possibility to covalently graft onto its residual quinone groups other nitrogen and thiol containing molecules through either Schiff base or Michael-type additions [23][24][25][26][27]. This possibility, together with a high reactivity and the possibility to cover an almost unlimited number of materials offer many unexplored opportunities for biosensor assemblies [23][24][25][26][27][28][29][30][31][32]. In fact, PDA provides a suitable microenvironment for immobilizing a high density of oriented biomolecules with their catalytic and biorecognition activity intact [23,25,[33][34][35][36][37].…”

Section: Introductionmentioning

confidence: 99%

Cholesterol biosensing with a polydopamine-modified nanostructured platinum electrode prepared by oblique angle physical vacuum deposition

Martín

Salazar

Álvarez

et al. 2017

Sensors and Actuators B: Chemical

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This paper reports a novel cholesterol biosensor based on nanostructured platinum (Pt) thin films prepared by Magnetron Sputtering (MS) in an oblique angle (OAD) configuration. Pt thin films were deposited onto a gold screen-printed electrode and characterized using Rutherford Back Scattering (RBS), Scanning Electron Microscopy (SEM), X-ray Diffraction (XRD), Cyclic Voltammetry (CV), X-ray Photo-electron Spectroscopy (XPS), Atomic Force Microscopy (AFM) and wetting analysis. Our results confirmed that the film is highly porous and formed by tilted nanocolumns, with an inclination of around 40º and a total thickness of 280 nm. XRD and CV analysis confirmed the polycrystalline nature of the Pt thin film. Cholesterol oxidase (ChOx) was covalently immobilized using a bioinspired polymer, polydopamine (PDA), via Schiff base formation and Michael-type addition. After being immobilized, ChOx displayed an apparent activation energy of 34.09 kJ mol-1 and a Michaelis constant (K M) equal to 3.65 mM, confirming the high affinity between ChOx and cholesterol and the excellent ability of the PDA film for immobilizing biological material without degradation. Under optimized working conditions our biosensor presented a sensitivity of 14.3 mA M −1 cm −2 (R 2 :0.999) with a linear range up to 0.5 mM and a limit of detection of 10.5 µM (S/N = 3). Furthermore the biosensor exhibits a fast response (< 8 s), good anti-interference properties and high stability after relatively long-term storage (2 months).

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Section: Introductionmentioning

confidence: 99%

Cholesterol biosensing with a polydopamine-modified nanostructured platinum electrode prepared by oblique angle physical vacuum deposition

Martín

Salazar

Álvarez

et al. 2017

Sensors and Actuators B: Chemical

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“…Mechanism of dopamine polymerization is reported by Jang et al [27]. Polymerization of dopamine can also be observed by the colour change from clear to darkish brown [28]. Figure 5 shows XRD patterns of manganese oxide and the nickel oxide.…”

Section: Modified Gold Electrodesmentioning

confidence: 70%

Nanowires Nickel Oxide and Nanospherical Manganese Oxide Synthesized via Low Temperature Hydrothermal Technique for Hydrogen Peroxide Sensor

Ibrahim

Charinpanitkul

Kobatake

et al. 2016

Journal of Chemistry

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Binary catalyst nickel oxides (NiO) and manganese oxides (MnO) were prepared individually via hydrothermal route. The catalysts were characterized by scanning electron microscope (SEM), Brunauer-Emmett-Teller (BET) analysis, cyclic voltammetry (CV), and amperometry. Morphology studies revealed physical structure of nanowires nickel oxide and spherical manganese oxide with estimated length of 0.3–2.3 μm and diameter of 0.2–0.8 μm, respectively. Surface areas obtained for nickel oxide and manganese oxide were 68.9 m2 g−1and 45.2 m2 g−1, respectively. Cyclic voltammetry exhibits electrochemical responses corresponding to the electrode surfaces. The linear responses of the binary catalyst modified gold electrodes with NiO-MnO were observed in the concentration range from 31.8 μM to 0.5 mM with the detection limit of 62.5 μM.

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“…By oxidizing dopamine diluted in phosphate buffered silane, polydopamine was formed, resulting in the immobilization of the DNAzymes onto the electrode’s surface. The resultant biosensor exhibited a limit of detection of 2.2 μM …”

Section: Dnazyme Immobilization Strategies For Biosensingmentioning

confidence: 99%

“…The resultant biosensor exhibited a limit of detection of 2.2 μM. 80 Ultimately, the nature of physical entrapment makes it difficult to implement in most sensing platforms, and its stability over time will be a challenge. The lack of existing literature using this strategy makes it difficult to characterize its effectiveness for DNAzyme immobilization.…”

Section: Dnazyme Immobilization Strategies For Biosensingmentioning

confidence: 99%

DNAzyme-Based Biosensors: Immobilization Strategies, Applications, and Future Prospective

et al. 2021

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Since their discovery almost three decades ago, DNAzymes have been used extensively in biosensing. Depending on the type of DNAzyme being used, these functional oligonucleotides can act as molecular recognition elements within biosensors, offering high specificity to their target analyte, or as reporters capable of transducing a detectable signal. Several parameters need to be considered when designing a DNAzyme-based biosensor. In particular, given that many of these biosensors immobilize DNAzymes onto a sensing surface, selecting an appropriate immobilization strategy is vital. Suboptimal immobilization can result in both DNAzyme detachment and poor accessibility toward the target, leading to low sensing accuracy and sensitivity. Various approaches have been employed for DNAzyme immobilization within biosensors, ranging from amine and thiol-based covalent attachment to non-covalent strategies involving biotin–streptavidin interactions, DNA hybridization, electrostatic interactions, and physical entrapment. While the properties of each strategy inform its applicability within a proposed sensor, the selection of an appropriate strategy is largely dependent on the desired application. This is especially true given the diverse use of DNAzyme-based biosensors for the detection of pathogens, metal ions, and clinical biomarkers. In an effort to make the development of such sensors easier to navigate, this paper provides a comprehensive review of existing immobilization strategies, with a focus on their respective advantages, drawbacks, and optimal conditions for use. Next, common applications of existing DNAzyme-based biosensors are discussed. Last, emerging and future trends in the development of DNAzyme-based biosensors are discussed, and gaps in existing research worthy of exploration are identified.

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An Electrochemical Hydrogen Peroxide Biosensor Based on Polydopamine-Entrapped G-Quadruplex-Hemin DNAzyme

Cited by 16 publications

References 18 publications

Cholesterol biosensing with a polydopamine-modified nanostructured platinum electrode prepared by oblique angle physical vacuum deposition

Cholesterol biosensing with a polydopamine-modified nanostructured platinum electrode prepared by oblique angle physical vacuum deposition

Nanowires Nickel Oxide and Nanospherical Manganese Oxide Synthesized via Low Temperature Hydrothermal Technique for Hydrogen Peroxide Sensor

DNAzyme-Based Biosensors: Immobilization Strategies, Applications, and Future Prospective

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