An electrochemical DNA hybridization detection assay based on a silver nanoparticle label

Cai, Hong; Xu, Ying; Zhu, Ning; He, Pingang; Fang, Yuzhi

doi:10.1039/b200555g

Cited by 193 publications

(99 citation statements)

References 27 publications

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“…Many kinds of nanoparticles, including metal nanoparticles, oxide nanoparticles, semiconductor nanoparticles, and even nanodimensional conducting polymers have been used in biosensors. For example, the use of gold and silver nanoparticles, or silver-silica hybrid nanostructures as biosensor substrates have been reported by several laboratories [4][5][6]. Some oxide nanoparticles and semiconductor nanoparticles such as MnO 2 nanoparticles [7] and CdS nanoparticles [8] are also applied to construct biosensor.…”

Section: Introductionmentioning

confidence: 99%

Gold nanoparticle-based biosensors

2010

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The unique properties of gold nanoparticles have stimulated the increasing interest in the application of GNPs in interfacing biological recognition events with signal transduction and in designing biosensing devices exhibiting novel functions. The optical properties of GNPs provide wide range opportunities for construction optical biosensors. The excellent biocompatibility, conductivity, catalytic properties and high surface-to-volume ratio and high density of GNPs facilitate extensive application of GNPs in construction of electrochemical and piezoelectric biosensors with enhanced analytical performance with respect to other biosensor designs. In this article, the recent advances in construction of GNP-based optical, electrochemical and piezoelectric biosensors are reviewed, and some illustrative examples given, with a focus on the roles GNPs play in the biosensing process and the mechanism of GNPs for improving the analytical performances. Finally, the review concludes with an outline of present and future research for the real-world applications.

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Section: Introductionmentioning

confidence: 99%

Gold nanoparticle-based biosensors

2010

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“…The peak position was related to the NaCl concentration by regression analysis in the semi-logarithmic plot (Figure 2 f). The degree of the peak shift was À10.1 mV per log 10 [NaCl] and À71.7 mV per log 10 [NaCl] at 0.01-1 mm and 1-50 mm NaCl, respectively. The results indicate the involvement of chloride ions in the oxidation of Ag NPs as Ag + Cl À !AgCl + e À and the oxidation in the solution without NaCl can be represented as Ag!Ag…”

Section: Resultsmentioning

confidence: 99%

Chemical Optimization for Simultaneous Voltammetric Detection of Molybdenum and Silver Nanoparticles in Aqueous Buffer Solutions

et al. 2014

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Simultaneous acquisition of redox signals from different types of metal nanoparticles (NPs) in a multiplexing system requires a good separation in redox potential from each component for successful identification. The appearance of a single distinct peak for each NP type is also preferred. Here, we report variations in the electrochemical nature of molybdenum (Mo) and silver (Ag) NPs cast on a glassy carbon electrode that are sensitive to various conditions of the measurement solution, such as buffering capacity and electrolyte concentration. A series of experiments allowed us to find optimal conditions, where Mo NPs showed a single oxidation peak with good separation of the redox potential from that of Ag NPs (ΔE≈0.25 V), by using differential‐pulse voltammetry (DPV). In the simultaneous monitoring of Mo and Ag NPs under the optimal conditions, the peak currents in DPV were quantitative for the amount of Mo/Ag NPs ranging from 7.8/20 to 500/20 ng ng−1 and from 500/20 to 500/0.31 ng ng−1.

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“…The EC transduction of the hybridization event relies typically on external electrochemically-active indicators, which comprise DNA intercalators (metal complexes, 24,25 anthracycline antibiotics, 26 and bisbenzimide dyes 27,28 ), enzymes, 29,30 and metal nanoparticles. [31][32][33] Despite the extensive research efforts in EC hybridization transduction, just a few groups, 28,29,34 including our group, have devoted to PCR product detection. Moreover, to the best of our knowledge, the integration of microchip-based PCR with EC detection functionality has not been reported yet.…”

Section: Introductionmentioning

confidence: 99%

Microfabricated PCR-electrochemical device for simultaneous DNA amplification and detection

2003

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Microfabricated silicon/glass-based devices with functionalities of simultaneous polymerase chain reaction (PCR) target amplification and sequence-specific electrochemical (EC) detection have been successfully developed. The microchip-based device has a reaction chamber (volume of 8 µl) formed in a silicon substrate sealed by bonding to a glass substrate. Electrode materials such as gold and indium tin oxide (ITO) were patterned on the glass substrate and served as EC detection platforms where DNA probes were immobilized. Platinum temperature sensors and heaters were patterned on top of the silicon substrate for real-time, precise and rapid thermal cycling of the reaction chamber as well as for efficient target amplification by PCR. DNA analyses in the integrated PCR-EC microchip start with the asymmetric PCR amplification to produce single-stranded target amplicons, followed by immediate sequence-specific recognition of the PCR product as they hybridize to the probe-modified electrode. Two electrochemistry-based detection techniques including metal complex intercalators and nanogold particles are employed in the microdevice to achieve a sensitive detection of target DNA analytes. With the integrated PCR-EC microdevice, the detection of trace amounts of target DNA (as few as several hundred copies) is demonstrated. The ability to perform DNA amplification and EC sequence-specific product detection simultaneously in a single reaction chamber is a great leap towards the realization of a truly portable and integrated DNA analysis system.

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An electrochemical DNA hybridization detection assay based on a silver nanoparticle label

Cited by 193 publications

References 27 publications

Gold nanoparticle-based biosensors

Gold nanoparticle-based biosensors

Chemical Optimization for Simultaneous Voltammetric Detection of Molybdenum and Silver Nanoparticles in Aqueous Buffer Solutions

Microfabricated PCR-electrochemical device for simultaneous DNA amplification and detection

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