An electrochemical DNA biosensor analytic technique for identifying DNA methylation specific sites and quantify DNA methylation level

Huang, Jian; Zhang, Shu; Mo, Fei; Su, Shasha; Chen, Xi; Li, Yan; Fang, Lichao; Huang, Hui; Deng, Jun; Liu, Hua‐Min; Yang, Xiaoli; Zheng, Junsong

doi:10.1016/j.bios.2018.12.022

Cited by 40 publications

(22 citation statements)

References 51 publications

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“…Several approaches for DNA analysis systems such as optical (fluorescent, surface plasmon resonance luminescent, colorimetric, etc.) [1][2][3][4][5], piezoelectric [6], or electrochemical [7][8][9] have been proposed. The electrochemical approach is promising because it can provide the above advantages whilst also offering the possibility of self-powered miniaturized devices relevant for point-of-care applications [10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Influence of Graphene Oxide Concentration when Fabricating an Electrochemical Biosensor for DNA Detection

et al. 2019

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We have investigated the influence exerted by the concentration of graphene oxide (GO) dispersion as a modifier for screen printed carbon electrodes (SPCEs) on the fabrication of an electrochemical biosensor to detect DNA hybridization. A new pretreatment protocol for SPCEs, involving two successive steps in order to achieve a reproducible deposition of GO, is also proposed. Aqueous GO dispersions of different concentrations (0.05, 0.1, 0.15, and 0.2 mg/mL) were first drop-cast on the SPCE substrates and then electrochemically reduced. The electrochemical properties of the modified electrodes were investigated after each modification step by cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS), while physicochemical characterization was performed by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), and Raman spectroscopy. Finally, the sensing platform was obtained by the simple adsorption of the single-stranded DNA probe onto the electrochemically reduced GO (RGO)-modified SPCEs under optimized conditions. The hybridization was achieved by incubating the functionalized SPCEs with complementary DNA target and detected by measuring the change in the electrochemical response of [Fe(CN)6]3–/4– redox reporter in CV and EIS measurements induced by the release of the newly formed double-stranded DNA from the electrode surface. Our results showed that a higher GO concentration generated a more sensitive response towards DNA detection.

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Section: Introductionmentioning

confidence: 99%

Influence of Graphene Oxide Concentration when Fabricating an Electrochemical Biosensor for DNA Detection

et al. 2019

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“…To observe the complementary base pairing and post translational modifications, secondary conjugates such as antibody or enzyme can be used in the DNA based biosensor studies. Huang et al [16] developed a biosensor to determine DNA methylation levels. In this study, the methylation level was determined with the help of a bioconjugate modified by a secondary antibody.…”

Section: Dna and Rna Usage In Biosensor Technologymentioning

confidence: 99%

Nucleic Acids for Electrochemical Biosensor Technology

Uygun¹,

Uygun²,

Sağın³

2021

Biosensors - Current and Novel Strategies for Biosensing

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Biosensor technology has developed extremely rapidly in recent years. This technology brings along precise measurements as well as specific measurements. Thanks to its ability to be miniaturized and be easily accessible to the end user, it is one-step ahead of other similar methods. The selectivity of biological molecules and the sensitivity of electrochemical methods enable the continuous evolvement of these new technologies. In this chapter, the use of nucleic acids as both recognition agents and target molecules, the way they are used in biosensor technology and their electrical properties are explained in detail with examples. Aptamers, which are synthetic nucleic acids, and their use in electrochemical biosensor systems with different electrochemical and immobilization methods have been compared extensively.

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“…DNA methylation, as one of the most widely studied epigenetic modifications, is a key factor in the regulation of gene expression during development and has been confirmed to be relevant to the pathogenesis of gene expression diseases and malignancies (X. Chen et al, 2019; J. Huang et al, 2019). Currently, an increasing number of methylated genes have been identified as significant biomarkers of tumorigenesis; thus, their detection has great significance for the diagnosis and prognosis of tumors (J. Huang et al, 2019). The CRISPR‐Cas9 triggered exponential amplification (CAS‐EXPAR) method has been developed as a powerful tool for the detection of site‐specific methylation of genomic DNA (Figure 2d; M. Huang, Zhou, Wang, & Xing, 2018).…”

Section: Detection Of Genomic Dnamentioning

confidence: 99%

Novel nucleic acid detection strategies based on CRISPR‐Cas systems: From construction to application

Zhu

Liu

Xie

et al. 2020

Biotech & Bioengineering

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Beyond their widespread application as genome‐editing and regulatory tools, clustered regularly interspaced short palindromic repeats (CRISPR)‐CRISPR‐associated (Cas) systems also play a critical role in nucleic acid detection due to their high sensitivity and specificity. Recently developed Cas family effectors have opened the door to the development of new strategies for detecting different types of nucleic acids for a variety of purposes. Precise and efficient nucleic acid detection using CRISPR‐Cas systems has the potential to advance both basic and applied biological research. In this review, we summarize the CRISPR‐Cas systems used for the recognition and detection of specific nucleic acids for different purposes, including the detection of genomic DNA, nongenomic DNA, RNA, and pathogenic microbe genomes. Current challenges and further applications of CRISPR‐based detection methods will be discussed according to the most recent developments.

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An electrochemical DNA biosensor analytic technique for identifying DNA methylation specific sites and quantify DNA methylation level

Cited by 40 publications

References 51 publications

Influence of Graphene Oxide Concentration when Fabricating an Electrochemical Biosensor for DNA Detection

Influence of Graphene Oxide Concentration when Fabricating an Electrochemical Biosensor for DNA Detection

Nucleic Acids for Electrochemical Biosensor Technology

Novel nucleic acid detection strategies based on CRISPR‐Cas systems: From construction to application

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