An electrochemical biosensor for the direct detection of oxytetracycline in mouse blood serum and urine

Abstract

Oxytetracycline (OTC), a broad-spectrum antibiotic, has been extensively used as a food additive for livestock. Its extensive use has greatly increased the risk of chronic drug abuse and has also increased the risk of the resulting diseases. Therefore, in light of this emerging situation, the detection of OTC in both food and livestock is very important to reduce the risks and for diagnosis purposes . In this work, we have proposed an electrochemical aptasensor to quantify OTC. The biosensor shows considerable sensitivity and selectivity, and it can be easily operated and regenerated. Furthermore, for the first time, we have shown that an electrochemical aptasensor can be directly used to detect OTC in mouse blood serum and urine. This biosensor has the potential to aid in the analysis of residual OTC levels, as well as providing more pharmacokinetic information in the future.

A colorimetric biosensor using Fe3O4 nanoparticles for highly sensitive and selective detection of tetracyclines

“…These results demonstrate that the proposed method can sensitively detect TCs. As shown in Tables 1, the value of the LOD was comparable to previously reported values obtained from the electrochemical, [29] high performance liquid chromatography, [30] paper-based color test-strip [31] and electrochemiluminescence biosensors. [32] The repeatability of the proposed method is evaluated by six repeated measurements of 500nM OTC, and the relative standard deviation is 1.5%, proving the reliability of the detection method.…”

Aptamer-based Biosensors for Antibiotic Detection: A Review

“…Zheng et al [201] developed an electrochemical aptasensor for direct detection of oxytetracycline in mouse blood, serum and urine. First, the working electrode was incubated with ferrocenefunctionalized cDNA of the aptamer and these formed a SAM onto the surface of the electrode via Au-S bonding.…”

Gold nanoparticle based photometric determination of tobramycin by using new specific DNA aptamers

“…Several aptamers targeting antibiotics such as streptomycin [20], tetracycline [21], chloramphenicol [22], ofloxacin [23] have been screened. Then these aptamers have been further applied in the detection of antibiotics using different techniques, such as electrochemical [24,25], colorimetric [26], fluorescent [27], chemiluminescent [28] and cantilever array [29] detection. Among those reported detections, gold nanoparticle (AuNP) attracted wide attention and was frequently used as the carrier of recognition elements or the probe to amplify the detection signal, due to its unique characteristics, including strong surface plasmon resonance absorption, high extinction coefficient, excellent electrical conductivity, huge specific surface area, and high biocompatibility [30].…”