An electrochemical biosensor for SARS-CoV-2 detection via its papain-like cysteine protease and the protease inhibitor screening

Liang, Qizhi; Huang, Yue; Wang, Minghui; Kuang, Deqi; Yang, Jingsi; Yi, Yongxiang; Shi, Hai; Li, Jinlong; Yang, Jie; Li, Genxi

doi:10.1016/j.cej.2022.139646

Cited by 27 publications

(6 citation statements)

References 59 publications

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“…The sensor exhibits a linear response in the range of 0–2500 copies/μL. Many technologies have been developed to detect COVID-19, which vary in their target, detection performance (Table S2), and cost. − Although many electrochemical-based devices are being developed to tackle the current pandemic, these technologies focus on the detection of COVID-19 antibodies which inherently provides lesser sensitivity and specificity. Currently, to the best of our knowledge, at the time of drafting this manuscript, there are no commercially translated electrochemical tests available for molecular detection of SARS-CoV-2.…”

Section: Resultsmentioning

confidence: 99%

Binding-Induced Folding of DNA Oligonucleotides Targeted to the Nucleocapsid Gene Enables Electrochemical Sensing of SARS-CoV-2

Alafeef

Skrodzki

Moitra

et al. 2023

ACS Appl. Bio Mater.

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In the wake of the COVID-19 pandemic, millions of confirmed cases and deaths have been reported around the world. COVID-19 spread can be slowed and eventually stopped by a rapid test to diagnose positive cases of the disease on the spot. It is still important to test for COVID-19 quickly regardless of the availability of the vaccine. Using the binding-induced folding principle, we developed an electrochemical test for detecting SARS-CoV-2 with no RNA extraction or nucleic acid amplification. The test showed high sensitivity with a limit of detection of 2.5 copies/μL. An electrode mounted with a capture probe and a portable potentiostat are used to conduct the test. To target the N-gene of SARS-CoV-2, a highly specific oligo-capturing probe was used. Based on the binding-induced “folding” principle, the sensor detects binding between the oligo and RNA. When the target is absent, the capture probe tends to form a hairpin as a secondary structure, retaining the redox reporter close to the surface. This can be seen as a large anodic and cathodic peak current. When the target RNA is present, the hairpin structure will open to hybridize with its complementary sequence, causing the redox reporter to pull away from the electrode. Consequently, the anodic/cathodic peak currents are reduced, indicating the presence of the SARS-CoV-2 genetic material. Validation of the test performance was performed using 122 COVID-19 clinical samples (55 positives and 67 negatives) and benchmarked to the gold standard reverse transcription-polymerase chain reaction (RT-PCR) test. As a result of our test, the accuracy, sensitivity, and specificity have been measured at 98.4%, 98.2%, and 98.5%, respectively.

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Section: Resultsmentioning

confidence: 99%

Binding-Induced Folding of DNA Oligonucleotides Targeted to the Nucleocapsid Gene Enables Electrochemical Sensing of SARS-CoV-2

Alafeef

Skrodzki

Moitra

et al. 2023

ACS Appl. Bio Mater.

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“…After immunoreaction, the MB molecules loaded in the bacteria can be released into solution and then captured by DNA preimmobilized on the electrode surface through electrostatic interaction and intercalation. , The successful immobilization of DNA duplex on the gold electrode surface has been verified, as shown in Figure S7. Subsequently, the feasibility of the fabricated Ec HER2 biocarrier-based immunosensor for HER2 detection is studied, and the current signal of MB is recorded using SWV.…”

Section: Validation Of the Electrochemical Immunosensor For Her2 Dete...mentioning

confidence: 99%

Engineered Escherichia coli as a Controlled-Release Biocarrier for Electrochemical Immunoassay

Zeng

Nie

et al. 2023

Nano Lett.

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Micro/nanocarriers hold great potential in bioanalysis for molecular recognition and signal amplification but are frequently hampered by harsh synthesis conditions and timeconsuming labeling processes. Herein, we demonstrate that Escherichia coli (Ec) can be engineered as an efficient biocarrier for electrochemical immunoassay, which can load ultrahigh amounts of redox indicators and simultaneously be decorated with detection antibodies via a facile polydopamine (PDA)mediated coating approach. Compared with conventional carrier materials, the entire preparation of the Ec biocarrier is simple, highly sustainable, and reproducible. Moreover, immune recognition and electrochemical transduction are performed independently, which eliminates the accumulation of biological interference on the electrode and simplifies electrode fabrication. Using human epidermal growth factor receptor 2 (HER2) as the model target, the proposed immunosensor exhibits excellent analytical performance with a low detection limit of 35 pg/mL. The successful design and deployment of Ec biocarrier may provide new guidance for developing biohybrids in biosensing applications.

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“…224 Except for the common RNA or antibody detection, he came up with a new method that used papain-like cysteine protease (PLpro) as a biomarker in detecting SARS-CoV-2, which can be used in real surroundings, including blood and saliva with a LOD of 27.18 fM. 225 Electrochemical SARS-CoV-2 sensors have the advantage of low-cost and commercially available systems. They can also achieve high sensitivity by using carefully designed sensing biointerfaces.…”

Section: Electrochemistrymentioning

confidence: 99%

Section: Electrochemistrymentioning

confidence: 99%

Nano-biosensor for SARS-CoV-2/COVID-19 detection: methods, mechanism and interface design

et al. 2023

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An electrochemical biosensor for SARS-CoV-2 detection via its papain-like cysteine protease and the protease inhibitor screening

Cited by 27 publications

References 59 publications

Binding-Induced Folding of DNA Oligonucleotides Targeted to the Nucleocapsid Gene Enables Electrochemical Sensing of SARS-CoV-2

Binding-Induced Folding of DNA Oligonucleotides Targeted to the Nucleocapsid Gene Enables Electrochemical Sensing of SARS-CoV-2

Engineered Escherichia coli as a Controlled-Release Biocarrier for Electrochemical Immunoassay

Nano-biosensor for SARS-CoV-2/COVID-19 detection: methods, mechanism and interface design

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