An electrochemical aptasensor with N protein binding aptamer-complementary oligonucleotide as probe for ultra-sensitive detection of COVID-19

Yu, Mengdi; Zhang, Mengjie; Zhang, Xin; Zahra, Qurat ul Ain; Huang, Zenghui; Chen, Ying; Song, Chunxia; Song, Min Suk; Jiang, Hongjuan; Z, Luo; Lu, Ying

doi:10.1016/j.bios.2022.114436

Cited by 15 publications

(7 citation statements)

References 43 publications

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“…Lu et al modified the N protein aptamer A48 on the surface of a gold electrode with ferrocene (Fc)-labeled complementary oligonucleotide. The concentration of N protein was detected by the change in the current caused by the structural change of Fc-complementary DNA with aptamer binding [55] (Figure 3e). The LOD of this strategy for N protein was 1 fM (Table 2).…”

Section: Sars-cov-2 Diagnostic Strategies Based On Aptamersmentioning

confidence: 99%

Aptamer‐based strategies against SARS‐CoV‐2 viruses

Huang

Chen

Zhang

et al. 2023

BMEMat

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The COVID‐19 pandemic, caused by SARS‐CoV‐2, has seriously threatened human life and security in recent years and has had a serious impact on economic development. A range of strategies, including antibodies, small‐molecule agents and vaccines, have been developed and widely used to combat SARS‐CoV‐2. However, the uncertain development and susceptibility to mutation of SARS‐CoV‐2 pose challenges to these approaches, making it necessary to develop a broader range of strategies to complement and expand the diversity of attacks against SARS‐CoV‐2. As promising tools against SARS‐CoV‐2, aptamers have attracted wide attention due to their unique molecular properties. In this review, we survey SARS‐CoV‐2 aptamers and aptamer‐based detection tools as well as aptamer‐based therapeutics. In addition, we analyze the potential value of aptamers in the investigation of SARS‐CoV‐2 infection mechanism and vaccine design. Finally, we look forward to the future development direction of aptamers against SARS‐CoV‐2 or other viruses.

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Section: Sars-cov-2 Diagnostic Strategies Based On Aptamersmentioning

confidence: 99%

Aptamer‐based strategies against SARS‐CoV‐2 viruses

Huang

Chen

Zhang

et al. 2023

BMEMat

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“…These results proved that quantitative measurements of SARS-CoV-2 coronavirus antigens in serum/plasma are beneficial to accurate and early diagnosis of COVID-19 and can provide a way to supplement the gold-standard nucleic-acid detection by filling the test gaps. Furthermore, the quantification of the N protein is superior to that of the spike protein because it can avoid the problems related to the mutation of the latter, resulting in more reliable detection [ 17 ].…”

Section: Introductionmentioning

confidence: 99%

“…Luo group [ 22 ] screened N protein aptamer with high affinity (the minimum K d value was 0.49 ± 0.05 nM) in 2020. It has been successfully applied as a recognition element for the ultra-sensitive assay of COVID-19 in an electrochemical aptasensor by our group [ 17 ] and holds a promising prospect in many other analytical technologies.…”

Section: Introductionmentioning

confidence: 99%

β-Cyclodextrin Polymer-Based Fluorescence Enhancement Strategy via Host–Guest Interaction for Sensitive Assay of SARS-CoV-2

Gao

Yang

Zhang

et al. 2023

IJMS

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Nucleocapsid protein (N protein) is an appropriate target for early determination of viral antigen-based severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We have found that β-cyclodextrin polymer (β-CDP) has shown a significant fluorescence enhancement effect for fluorophore pyrene via host–guest interaction. Herein, we developed a sensitive and selective N protein-sensing method that combined the host–guest interaction fluorescence enhancement strategy with high recognition of aptamer. The DNA aptamer of N protein modified with pyrene at its 3′ terminal was designed as the sensing probe. The added exonuclease I (Exo I) could digest the probe, and the obtained free pyrene as a guest could easily enter into the hydrophobic cavity of host β-CDP, thus inducing outstanding luminescent enhancement. While in the presence of N protein, the probe could combine with it to form a complex owing to the high affinity between the aptamer and the target, which prevented the digestion of Exo I. The steric hindrance of the complex prevented pyrene from entering the cavity of β-CDP, resulting in a tiny fluorescence change. N protein has been selectively analyzed with a low detection limit (11.27 nM) through the detection of the fluorescence intensity. Moreover, the sensing of spiked N protein from human serum and throat swabs samples of three volunteers has been achieved. These results indicated that our proposed method has broad application prospects for early diagnosis of coronavirus disease 2019.

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“…Therefore, the development of ICA readers is still required and some special readers have been reported [ 35 , 36 , 37 ]. Electrochemical biosensors for detection of the SARS-CoV-2 N-protein have also been reported [ 38 , 39 , 40 , 41 , 42 , 43 , 44 ]. However, the requirement of a reference electrode in electrochemical biosensors may become a weak point as the liquid junction error often exists in practical situations [ 45 ].…”

Section: Introductionmentioning

confidence: 99%

Integration of Power-Free and Self-Contained Microfluidic Chip with Fiber Optic Particle Plasmon Resonance Aptasensor for Rapid Detection of SARS-CoV-2 Nucleocapsid Protein

Chang

Sun²,

Huang³

et al. 2022

Biosensors

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The global pandemic of COVID-19 has created an unrivalled need for sensitive and rapid point-of-care testing (POCT) methods for the detection of infectious viruses. For the novel coronavirus SARS-CoV-2, the nucleocapsid protein (N-protein) is one of the most abundant structural proteins of the virus and it serves as a useful diagnostic marker for detection. Herein, we report a fiber optic particle plasmon resonance (FOPPR) biosensor which employed a single-stranded DNA (ssDNA) aptamer as the recognition element to detect the SARS-CoV-2 N-protein in 15 min with a limit of detection (LOD) of 2.8 nM, meeting the acceptable LOD of 106 copies/mL set by the WHO target product profile. The sensor chip is a microfluidic chip based on the balance between the gravitational potential and the capillary force to control fluid loading, thus enabling the power-free auto-flowing function. It also has a risk-free self-contained design to avoid the risk of the virus leaking into the environment. These findings demonstrate the potential for designing a low-cost and robust POCT device towards rapid antigen detection for early screening of SARS-CoV-2 and its related mutants.

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An electrochemical aptasensor with N protein binding aptamer-complementary oligonucleotide as probe for ultra-sensitive detection of COVID-19

Cited by 15 publications

References 43 publications

Aptamer‐based strategies against SARS‐CoV‐2 viruses

Aptamer‐based strategies against SARS‐CoV‐2 viruses

β-Cyclodextrin Polymer-Based Fluorescence Enhancement Strategy via Host–Guest Interaction for Sensitive Assay of SARS-CoV-2

Integration of Power-Free and Self-Contained Microfluidic Chip with Fiber Optic Particle Plasmon Resonance Aptasensor for Rapid Detection of SARS-CoV-2 Nucleocapsid Protein

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