An electrochemical aptasensor for streptomycin based on covalent attachment of the aptamer onto a mesoporous silica thin film-coated gold electrode

A wrinkled structure of gold film greatly improves the signaling of electrochemical aptamer-based biosensors

“…Therefore, typical signaling currents from E-AB sensors that are small enough to insert in vivo are on the scale of microamperes or even nanoamperes. [17][18][19][20] This, in retrospect, limits the resolutions of sensors to determine small changes in target concentration. In order to enhance the signaling current of E-AB sensors, we and others have previously developed a set of electrochemical protocols that produce nely-controllable nanostructures on gold rod electrodes, [21][22][23][24] thus signicantly enhancing their microscopic surface areas.…”

Multimodal/Multifunctional Nanomaterials in (Bio)electrochemistry: Now and in the Coming Decade

“…MSF appears as an appealing nanosupport to design stable electrochemical biosensors due to its large surface area-to-volume ratio, well-ordered pore structure, uniform pore size, and easy modification due to the large content of hydroxyl groups at the surface [ 176 ]. Growing of MSFs on the electrode surface offers an inimitable array of perpendicular nanochannels with excellent permeability properties and unique structural characteristics [ 177 ]. Moreover, these nanopores can be rationally tuned by chemical transformation at the outer or inner surface and, therefore, modulate the specific properties of MSF-electrodes, such as charge, selective permeability, hydrophobicity/hydrophilicity, and electrocatalysis [ 175 ].…”

“…Moreover, these nanopores can be rationally tuned by chemical transformation at the outer or inner surface and, therefore, modulate the specific properties of MSF-electrodes, such as charge, selective permeability, hydrophobicity/hydrophilicity, and electrocatalysis [ 175 ]. For example, the conductivity of MSF can be improved by functionalizing with AgNPs [ 177 ]. Table 4 summarizes selected representative examples of electrochemical biosensing methods using multifunctional silica nanomaterials.…”

“…Regarding MSFs, to date, they have always been used as a modifier of conventional gold electrodes in catalytic or affinity biosensing methods to target transglutaminase activity (TGase) [ 196 ] Cu 2+ /ascorbic acid (AA) [ 195 ], E. coli 16S rRNA [ 175 ], prostate-specific antigen (PSA) [ 176 ], and streptomycin (STR) [ 177 ]. Villalonga’s group proposed an electrochemical assay to quantify TGase activity via the enzyme-controlled diffusion of Fe(CN) 6 3−/4− through amino-functionalized nanochannels of a MSF-AuE [ 196 ].…”

Electrochemical Immunosensors for Antibiotic Detection

“…A vast number of papers pertaining to sensors have been developed for electrochemical antibiotic determination [25,26,27]. Optical and electrochemical biosensors, including immunosensors [28,29] and aptasensors [30,31] as well as lateral flow assays [32,33], were also utilized for this purpose.…”