An electrochemical aptasensor for detection of bovine interferon gamma

Crulhas, Bruno P.; Hadley, Dustin J.; Liu, Ying; Shin, Dong‐Sik; Stybayeva, Gulnaz; Imanbekova, Meruyert; Hill, Ashley E.; Pedrosa, Valber A.; Revzin, Alexander

doi:10.1039/c7ay01313b

Cited by 13 publications

(8 citation statements)

References 21 publications

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“…AR signal suppression (SS) (Figure 4(b)) increased linearly with the increasing of AR concentration (SS = 9.23 x -7.535 (R 2 = 0.99)). Detection limit found was calculated around 0.5 ng/mL with a linear range from 0 to 110 ng/mL; such range for detection is lined up with AR concentrations on human prostate tissue demonstrating the possibility of using the rGO-AuNPs-aptamer sensor in order to detect AR on biological samples [48].…”

Section: Resultsmentioning

confidence: 65%

Reduced Graphene Oxide Decorated with AuNPs as a New Aptamer-Based Biosensor for the Detection of Androgen Receptor from Prostate Cells

et al. 2019

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Here we described an aptasensor based on graphene oxide (GO) decorated with gold nanoparticles (AuNPs) able to detect the AR released by prostate cells, in order to provide new insights about the relationship between AR and prostate cancer for a more precise diagnosis for cancer. Characterization of the surface modified GE was carried out by cyclic voltammetry and electrochemical impedance spectroscopy using ferrocene as a redox probe. Under the optimized experimental conditions, a detection limit of 0.5 ng/mL (3σ/slope) was obtained by the present electrochemical biosensor, along with a linear range of 0-110 ng/mL. By virtue of excellent sensitivity, specificity, and repeatability, the present electrochemical biosensor provides a potential application in clinical diagnosis.

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Section: Resultsmentioning

confidence: 65%

Reduced Graphene Oxide Decorated with AuNPs as a New Aptamer-Based Biosensor for the Detection of Androgen Receptor from Prostate Cells

et al. 2019

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“…Figure A shows the stability of the developed sensor over time: background signal was very stable and it did not cause significant loss in the peak current (around 10 % signal loss) as shown in Figure B. This outcome is similar to results recorded for a whole range of new electrochemical aptamer‐based biosensors .…”

Section: Resultsmentioning

confidence: 99%

Using an Electrochemical Aptasensor to Early Detect Prostate Specific and Free Prostate Specific Antigens Released by Cancer Cells

et al. 2018

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The detection of prostate cancer (PCa) in its early stage is essential to prevent metastasis. Patients who start their treatment earlier have greater chances to be cured; therefore, several research groups have been developing a simple and non‐invasive methodology to detect PCa. The aim of this article is to report a new method to detect prostate specific (PSA) and free prostate‐specific antigens (PSA) released by cancer cells based on the use of an aptasensor. Scanning electron microscopy (SEM), EDS and cyclic voltammetry were the instruments adopted to assess the surface of the biosensor. According to our results, the aptasensor was highly sensitive and recorded linear response 1–100 ng/ml of PSA and fPSA and detection limit 1.1 ng/ml. Studies on interfering compounds such as human serum protein, ascorbic acid, uric acid and mucin type I have shown low correlation to the used aptamer. Thus, this biosensor could be adopted in the near future to early diagnose PCa.

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“…The IFN-γ bonding triggered the aptamer hairpin to unfold, displacing the redox MB molecules away from the electrode and lowering the electron transfer efficacy as measured by SWV. The bovine gamma interferon (BoIFN-γ) was also assayed [ 102 ]. A graphene-based FET monolayer structure is integrated on a PDMS substrate with an IFN-γ aptamer attached to the graphene [ 103 ].…”

Section: Electrochemical Aptasensors For Clinical Applicationmentioning

confidence: 99%

Electrochemical Aptasensors: Current Status and Future Perspectives

Abd-Ellatief

2021

Diagnostics

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This article reviews the progress of diversity of electrochemical aptasensor for target analytes detection. The immobilization strategies of aptamers on an electrode surface are addressed. The aptasensors are also introduced in compliance with the assay platforms. Many electrochemical aptasensors are nearly identical to conventional immunochemical approaches, sandwich and competition assays using electroactive signaling moieties. Others are “signal-on” and “sign-off” aptasensors credited to the target binding-induced conformational change of aptamers. Label-free aptasensors are also highlighted. Furthermore, the aptasensors applied for clinically important biomarkers are emphasized.

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An electrochemical aptasensor for detection of bovine interferon gamma

Abstract: Bovine tuberculosis (TB) is an infectious disease caused byMycobacterium tuberculosis(MTB) in which it is hard to identify the pathological symptoms.

Cited by 13 publications

References 21 publications

Reduced Graphene Oxide Decorated with AuNPs as a New Aptamer-Based Biosensor for the Detection of Androgen Receptor from Prostate Cells

Reduced Graphene Oxide Decorated with AuNPs as a New Aptamer-Based Biosensor for the Detection of Androgen Receptor from Prostate Cells

Using an Electrochemical Aptasensor to Early Detect Prostate Specific and Free Prostate Specific Antigens Released by Cancer Cells

Electrochemical Aptasensors: Current Status and Future Perspectives

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