An elasticity microscope. Part II: Experimental results

Cohn, N.A.; Emelianov, Stanislav; O’Donnell, Matthew

doi:10.1109/58.656635

Cited by 42 publications

(16 citation statements)

References 6 publications

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“…The methods for preparation have been described in greater detail previously. 4,5 In brief, oral mucosa keratinocytes were enzymatically dissociated from the tissue sample, and a primary cell culture was established and propagated in a chemically defined, serum- and xenogeneic products-free culture medium, with calcium concentration of 1.2 mM. The AlloDerm ® was soaked in 5 µg/cm 2 human type IV collagen at 4 °C overnight prior to seeding cells to assist the adherence of cells, then approximately 1.5 × 10 5 cells/cm 2 of oral keratinocytes were seeded onto the type IV collagen pre-soaked AlloDerm ® .…”

Section: Methodsmentioning

confidence: 99%

Acoustic Microscopy Analyses to Determine Good vs. Failed Tissue Engineered Oral Mucosa Under Normal or Thermally Stressed Culture Conditions

et al. 2010

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This study uses scanning acoustic microscopy (SAM) ultrasonic profilometry to determine acceptable vs. failed tissue engineered oral mucosa. Specifically, ex vivo-produced oral mucosal equivalents (EVPOMEs) under normal or thermally stressed culture conditions were scanned with the SAM operator blinded to the culture conditions. As seeded cells proliferate, they fill in and smooth out the surface irregularities; they then stratify and produce a keratinized protective upper layer. Some of these transformations could alter backscatter of ultrasonic signals and in the case of the thermally stressed cells, produce backscatter similar to an unseeded device. If non-invasive ultrasonic monitoring could be developed, then tissue cultivation could be adjusted to measure biological variations in the stratified surface. To create an EVPOME device, oral mucosa keratinocytes were seeded onto acellular cadaveric dermis. Two sets of EVPO-MEs were cultured: one at physiological temperature 37 °C and the other at 43 °C. The specimens were imaged with SAM consisting of a single-element transducer: 61 MHz center frequency, 32 MHz bandwidth, 1.52 f#. Profilometry for the stressed and unseeded specimens showed higher surface irregularities compared to unstressed specimens. Elevated thermal stress retards cellular differentiation, increasing root mean square values; these results show that SAM can potentially monitor cell/tissue development.

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Section: Methodsmentioning

confidence: 99%

Acoustic Microscopy Analyses to Determine Good vs. Failed Tissue Engineered Oral Mucosa Under Normal or Thermally Stressed Culture Conditions

et al. 2010

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“…The displacement estimates were then accumulated over the entire data set spanning 800 µm surface applied deformation. Finally, the axial strain values were obtained by calculating the 1-D central derivative of the axial displacement estimates [52][53][54][55].…”

Section: Methodsmentioning

confidence: 99%

“…Therefore, ultrasound based strain imaging is also performed simultaneously with photoacoustic imaging [45,50,51]. Strain imaging is based on the analysis of ultrasound images captured during mechanical deformation of the tissue [52][53][54][55], i.e., the internal tissue motion and, therefore, strain is measured using speckle tracking algorithms. Therefore, qualitative measurements of blood perfusion can be obtained from ultrasound based strain and photoacoustic imaging.…”

Section: Introductionmentioning

confidence: 99%

Measurement of blood perfusion using photoacoustic, ultrasound, and strain imaging

Mallidi

Karpiouk

Aglyamov

et al. 2007

Photons Plus Ultrasound: Imaging and Sensing 2007: The Eighth Conference on Biomedical Thermoacoustics, Optoacoustics, and Acou

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In many clinical and research applications including cancer diagnosis, tumor response to therapy, reconstructive surgery, monitoring of transplanted tissues and organs, and quantitative evaluation of angiogenesis, sequential and quantitative assessment of microcirculation in tissue is required. In this paper we present an imaging technique capable of spatial and temporal measurements of blood perfusion through microcirculation. To demonstrate the developed imaging technique, studies were conducted using phantoms with modeled small blood vessels of various diameters positioned at different depths. A change in the magnitude of the photoacoustic signal was observed during vessel constriction and subsequent displacement of optically absorbing liquid present in the vessels. The results of the study suggest that photoacoustic, ultrasound and strain imaging could be used to sequentially monitor and qualitatively assess blood perfusion through microcirculation.

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“…The spatial resolution was estimated using the FWHM of the autocorrelation of the difference between strain maps estimated using sT and strain maps calculated using FEM, as described in [31].…”

Section: Rf Simulation and Rf-based Speckle Trackingmentioning

confidence: 99%

Estimation of shear modulus ratio between inclusion and background using strain ratios in 2-D ultrasound elastography

Jia

Alam

Azar

et al. 2014

IEEE Trans. Ultrason., Ferroelect., Freq. Contr.

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The goal of this study is to assess the effects of region of interest (ROI) selection and lesion size on estimates of shear modulus ratio from strain ratios to quantify relative stiffness of breast tumors. A theoretical model and finite element method (FEM) simulations of lesions with various shear modulus ratios are created for a 2-D plane strain deformation. Both the lesion and the surrounding tissue are assumed to be linearly elastic, isotropic, homogenous, and incompressible. The results from the model and simulations are in agreement that the lesion-to-surrounding shear modulus ratio is linearly proportional to the axial normal strain ratio for small lesions when the ROI in the surrounding tissue is at least four lesion diameters away from the lesion. For larger lesions, FEM simulations show that the estimated strain ratio using the same ROI location increases with the lesion size and would overestimate the shear modulus ratio. Therefore, a correction factor is necessary for large breast lesions when strain ratios are used to estimate the shear modulus ratio. We also demonstrate that strain elastograms calculated using a speckle tracking method on simulated RF data are accurate enough to observe the same effect on strain ratio estimation. This result is confirmed using experimental data acquired from two tissue-mimicking phantoms. Our findings will help clinicians to estimate strain ratios and shear modulus ratios more accurately for more reliable comparison of one clinical examination to another.

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An elasticity microscope. Part II: Experimental results

Cited by 42 publications

References 6 publications

Acoustic Microscopy Analyses to Determine Good vs. Failed Tissue Engineered Oral Mucosa Under Normal or Thermally Stressed Culture Conditions

Acoustic Microscopy Analyses to Determine Good vs. Failed Tissue Engineered Oral Mucosa Under Normal or Thermally Stressed Culture Conditions

Measurement of blood perfusion using photoacoustic, ultrasound, and strain imaging

Estimation of shear modulus ratio between inclusion and background using strain ratios in 2-D ultrasound elastography

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