Acoustic Microscopy Analyses to Determine Good vs. Failed Tissue Engineered Oral Mucosa Under Normal or Thermally Stressed Culture Conditions

Winterroth, Frank; Lee, Junho; Kuo, Shiuhyang; Fowlkes, J. Brian; Feinberg, Stephen E.; Hollister, Scott J.; Hollman, Kyle W.

doi:10.1007/s10439-010-0176-2

Cited by 9 publications

(6 citation statements)

References 20 publications

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“…Diminished cyclic variation may perhaps depend on less change in sound speed during a cardiac cycle because of incomeplete protein content of myocytes. Recently, several researchers have focused on sound speed of living cells and sought to utilize this technology in several clinical fields including in vivo tissues and cultured cells [31][32][33]. If modern technology allows development of the acoustic microscope system for use in a catheter-based system such as intracardiac ultrasound, new knowledge may be obtained regarding cyclic variation of myocyte sound speed, leading to diagnosis of genetic cardiomyopathy in vivo.…”

Section: Discussionmentioning

confidence: 99%

A new diagnostic feasibility for cardiomyopathy utilizing acoustic microscopy

Nakamura¹,

Kusano²,

Nakamura³

et al. 2013

WJCD

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Aims: Dilated cardiomyopathy often shows left ventricular systolic dysfunction, although histologically it always exhibits non-specific abnormality. We hypothesized that myocyte sound speed might be altered due to incomplete protein accumulation in cells. Methods and Results: Ninety eight biopsied samples were obtained from 49 patients comprising 43 with clinical dilated cardiomyopathy and 6 with hypertrophic cardiomyopathy. Sound speed was evaluated in deparaffinized 10 μm thick sections using an acoustic microscope (frequency range: 50 - 105 MHz). Conventional histology revealed 7 cases of persistent myocarditis derived from clinical dilated cardio- myopathy samples. Histology of the remaining dilated cardiomyopathy patients indicated non-specific abnormality. All hypertrophic cardiomyopathy cases exhibited myocardial disarray. Ten normal autopsied hearts were compared as controls. The sound speed of controls was 1627 ± 30m/sec. The sound speed in dilated cardiomyopathy samples (1700 ±51m/sec) was 1.045-fold faster compared to controls. The sound speed in hypertrophic cardiomyopathy samples (1734 ±51m/sec, 1.066-fold compared to controls) was faster than that of the myocarditis group (1672 ±30m/sec, 1.028-fold) (P = 0.0218). Furtheremore, desmin expression was evaluated as extent of emergence (grading 0 - 4). The desmin expression score in hypertrophic cardiomyopathy samples (2.7 ± 0.8) was significantly higher than in other groups (dilated 2.0 ± 1.4, myocarditis 1.6 ± 1.5 vs., controls 0, P ≤ 0.0001, 0.0001, 0.0129, respectively). Conclusion: Cardio-myopathy enhanced the sound speed, which correlated with the elasticity of myocytes, following the impaired compliance of left ventricle, despite the absence of histological changes. The elevation of sound speed of myocytes may be linked to cytoskeletal changes. Myocyte sound speed may be a new diagnostic tool for diagnosis of idiopathic cardiomyopathy independently of conventional histological diagnosis.

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Section: Discussionmentioning

confidence: 99%

A new diagnostic feasibility for cardiomyopathy utilizing acoustic microscopy

Nakamura¹,

Kusano²,

Nakamura³

et al. 2013

WJCD

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“…18 Acoustic microscopy techniques (at 61 MHz), have been used to characterize the development and surface irregularities of engineered human oral mucosal constructs. 19,20 …”

Section: B-mode Ultrasound Imagingmentioning

confidence: 99%

Quantitative Ultrasound for Nondestructive Characterization of Engineered Tissues and Biomaterials

2015

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Non-invasive, non-destructive technologies for imaging and quantitatively monitoring the development of artificial tissues are critical for the advancement of tissue engineering. Current standard techniques for evaluating engineered tissues, including histology, biochemical assays and mechanical testing, are destructive approaches. Ultrasound is emerging as a valuable tool for imaging and quantitatively monitoring the properties of engineered tissues and biomaterials longitudinally during fabrication and post-implantation. Ultrasound techniques are rapid, non-invasive, non-destructive and can be easily integrated into sterile environments necessary for tissue engineering. Furthermore, high-frequency quantitative ultrasound techniques can enable volumetric characterization of the structural, biological, and mechanical properties of engineered tissues during fabrication and post-implantation. This review provides an overview of ultrasound imaging, quantitative ultrasound techniques, and elastography, with representative examples of applications of these ultrasound-based techniques to the field of tissue engineering.

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“…LIPUS has proven to be a clinically established, widely used and FDA approved therapy to enhance bone growth during healing of non-union fractures and other osseous defects (32, 33). Various studies have also shown that ultrasound can control the rate of scaffold degradation (13, 34-35-36), improve scaffold integration, and that they modulate different specific cellular aspects (14, 29, 37-38-39-40-41-42). Recently, our group demonstrated that LIPUS with spatial averaged and temporal averaged (SATA) intensity of 30 mW/cm 2 is able (i) to maintain hMSCs stemness in vitro for up to 28 days and therefore guarantee the presence of a stem cells reservoir and (ii) to enhance and accelerate the osteogenic differentiation of hMSCs, thereby inducing the release of a master regulator of the angiogenic process (VEGF) (unpublished data).…”

Section: Introductionmentioning

confidence: 99%

Effect of Low-Intensity Pulsed Ultrasound on Osteogenic Human Mesenchymal Stem Cells Commitment in a New Bone Scaffold

Carina

Costa

Raimondi

et al. 2017

Journal of Applied Biomaterials & Functional Materials

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Purpose Bone tissue engineering is helpful in finding alternatives to overcome surgery limitations. Bone growth and repair are under the control of biochemical and mechanical signals; therefore, in recent years several approaches to improve bone regeneration have been evaluated. Osteo-inductive biomaterials, stem cells, specific growth factors and biophysical stimuli are among those. The aim of the present study was to evaluate if low-intensity pulsed ultrasound stimulation (LIPUS) treatment would improve the colonization of an MgHA/Coll hybrid composite scaffold by human mesenchymal stem cells (hMSCs) and their osteogenic differentiation. LIPUS stimulation was applied to hMSCs cultured on MgHA/Coll hybrid composite scaffold in osteogenic medium, mimicking the microenvironment of a bone fracture. Methods hMSCs were seeded on MgHA/Coll hybrid composite scaffold in an osteo-inductive medium and exposed to LIPUS treatment for 20 min/day for different experimental times (7 days, 14 days). The investigation was focused on (i) the improvement of hMSCs to colonize the MgHA/Coll hybrid composite scaffold by LIPUS, in terms of cell viability and ultrastructural analysis; (ii) the activation of MAPK/ERK , osteogenic ( ALPL , COL1A1 , BGLAP , SPP1 ) and angiogenetic ( VEGF , IL8) pathways, through gene expression and protein release analysis, after LIPUS stimuli. Results LIPUS exposure improved MgHA/Coll hybrid composite scaffold colonization and induced in vitro osteogenic differentiation of hMSCs seeded on the scaffold. Conclusions This work shows that the combined use of new biomimetic osteo-inductive composite and LIPUS treatment could be a useful therapeutic approach in order to accelerate bone regeneration pathways.

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Acoustic Microscopy Analyses to Determine Good vs. Failed Tissue Engineered Oral Mucosa Under Normal or Thermally Stressed Culture Conditions

Cited by 9 publications

References 20 publications

A new diagnostic feasibility for cardiomyopathy utilizing acoustic microscopy

A new diagnostic feasibility for cardiomyopathy utilizing acoustic microscopy

Quantitative Ultrasound for Nondestructive Characterization of Engineered Tissues and Biomaterials

Effect of Low-Intensity Pulsed Ultrasound on Osteogenic Human Mesenchymal Stem Cells Commitment in a New Bone Scaffold

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