An Effort of Mutation Breeding by Oryzalin and Gamma Rays on Wild Raspberry (Rubus sp.) in Cibodas Botanical Garden

Surya, Muhammad Imam; Ismaini, Lily; Destri, Destri; Normasiwi, Suluh

doi:10.15294/biosaintifika.v8i3.6559

Cited by 4 publications

(3 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Research related to mutation induction effect on the content of plant secondary metabolite compounds was carried out at Dendrobium officinale (Pham et al, 2019), Solanum bulbocastanum (Caruso et al, 2013), Physalis peruviana L. (Çömlekçioğlu & Özden, 2020), Melissa officinalis (Talei & Fotokian, 2020), Capsicum frutescens (Pliankong et al, 2017), Artemisia cina (Kasmiyati et al, 2020) and Cnidium officinale (Kim et al, 2021). Oryzalin is a safer mutation agent because it does not inhibit the vegetative growth of plants (Surya et al, 2016). Mutation induction using oryzalin in local Tawangmangu stevia plants is expected to increase the diversity of stevia plants with properties different from those of diploid stevia plants.…”

Section: Introductionmentioning

confidence: 99%

Stevia Local Tawangmangu Generation M1 Result of Oryzalin Treatment

Susanti,

Parjanto,

Haryanti

2024

Jurnal Biodjati

View full text Add to dashboard Cite

Stevia rebaudiana Bert. (Stevia) is used in the commercial and health industries because of its steviol glycosides (stevioside, rebaudioside A, rebaudioside C) and flavonoids. Polyploidy induction of stevia plants using oryzalin was held to increase the diversity and produce superior varieties. This research aims to obtain stevia diversity with different characteristics from its diploid. The Tawangmangu variety of stevia seedlings was experimentally designed using a completely random design. Nine combinations of oryzalin concentrations (1.5, 2.5, and 3.5μM) and immersion times (4, 6, and 8 hours) treatments were applied to 15 stevia seedlings each. Stevia without treatment was used as a control. Treatments were applied directly to the shoots on the second internode from the tip. Observations on target shoots included the number of survivors, morphological variables (height, number of internodes, internode length, leaf size, leaf thickness, stem diameter, and leaf color), stomata, and plant ploidy level. Data analysis on the number of live plants used frequency and descriptively. Morphological data consisting of plant height, number of shoots, number of internodes, internode length, leaf length, leaf width, leaf thickness, and stem diameter were analyzed using boxplot graphs and descriptive to describe the diversity of M1 stevia treatment results. Leaf color and stomata were analyzed descriptively. The results showed that up to 3.5μM concentrations of oryzalin and 6 hours of immersion time were safe to use as a mutation agent with above 67% survival rate. Various oryzalin treatments of Tawangmangu stevia varieties yielded polyploidy morphological growth indications in height, number of internodes, internode length, stem diameter, leaf size, leaf thickness, leaf color, stomata, and stem diameter. In addition, there are growth variations such as chimeras, rosettes, and leaf splitting. However, further flow cytometry tests showed that oryzalin concentration and immersion duration directly on the vegetative material did not produce polyploid stevia individuals.

show abstract

Section: Introductionmentioning

confidence: 99%

Stevia Local Tawangmangu Generation M1 Result of Oryzalin Treatment

Susanti,

Parjanto,

Haryanti

2024

Jurnal Biodjati

View full text Add to dashboard Cite

show abstract

“…Irradiating gamma rays on Phalaenopsis amabilis plantlets that are ready to be acclimatized and getting a radiation dose of 25 Gy (Widiarsih & Dwimahyani, 2013). Surya et al (2016) EMS is of chemical mutagen which can trigger alkylation and is most likely to cause gene mutations (Talebi et al, 2012). According to Li et al (2021), there have been no studies related to mutations caused by chemical mutagens belonging to alkylating agents that cause high heterozygosity in orchids.…”

Section: Introductionmentioning

confidence: 99%

Induction of Protocorm-Like Bodies (PLBs) Phalaenopsis spp. Hybrids Mutation through Ultraviolet Irradiation (UV254) and Ethyl Methane Sulfonate (EMS)

et al. 2023

View full text Add to dashboard Cite

Phalaenopsis sp. is the most-produced orchid species in Indonesia. Compared to conventional breeding, mutation induction by using mutagens, such as Ultraviolet Light-C (λ = 254 nm) (UV254) and Ethyl Methane Sulfonate (EMS), could probably result in new superior orchid variants. This research aims to get some mutants with phenotypes that have visual differences in the Phalaenopsis spp. hybrids wild type. There were 4 durations of UV254 irradiation: 5’ on, 85’ off; 10’ on, 80’ off (1 day and 7 days for each treatment); 4 concentrations of EMS used in this research: 0.05%; 0.06%; 0.07%; 0.08% for 6 hours of immersion; selected UV254 irradiation (5’ on, 85’ off (7 days)) combined with these concentrations. UV254 irradiation treatment (5’ on, 85’ off (1 day and 7 days); 10’ on, 80’ off (7 days)) resulted in some mutants with leaf phenotypes that were visually different from the wild type; 0.05% EMS treatment resulted in PLBs mutant with a visually larger size than the wild type; 0.08% EMS treatment and combination treatments (for EMS 0.05% and 0.08% for each treatment) resulted in non-growing albino PLBs. Hence, mutation induction using UV254 and EMS in this research produced several most likely mutants having visual differences that may be more desirable than the wild type.

show abstract

“…It is also important to genetic improvement of plant species which is achieved by selection, first from population in fields that continued unique genotype fixed by vegetative propagation (Surya et al,2016). Maturity and storage of seed need to be attended correlating to seed quality for seed propagation.…”

Section: Introductionmentioning

confidence: 99%

Effect of Seed Maturity and Storage Duration on Germination of Sambiloto (Andrographis paniculata (Burm.f.) Nees)

Solikin

Nurfadilah

2017

J Bio Bio Edu

View full text Add to dashboard Cite

Seed maturity and its storage are one of problems on propagation and developing of medicinal plants such as sambiloto (Andrographis paniculata (Burm.f.) ex Nees). The research aimed to determine effects of seed maturity and storage duration on seed germination of sambiloto was conducted in a green house of Purwodadi Botanical Garden from November 2014 to November 2015. The experiment was done by completely randomised design with two treatments namely seed maturity and storage duration. The seed was classified into immature seed (0.061 g/100 seeds), semimature seed (0.113 g/100 seeds) and mature seed (0.166 g/100 seeds). The seed's storage duration was classified into seed was not stored, seed was stored for six months and seed was stored for twelve months. Each treatment combination was replicated five times. The results showed that there were significant interactions between the treatments on percentage and rate of seed germination. The highest percentage of seed germination was the treatment of mature seed and stored twelve months (98.50%). On the contrast, the lowest seed germination percentage was the treatment of immature seed and stored twelve months (4.25 %). The fastest seed germination rate was the treatment of mature seed and stored six months (3.88 days), whereas the slowest seed germination rate was the treatment of immature seed and without stored (28.58 days). This study is expected to be applied to improve genetic and cultivation of medicinal plant as well as increasing plant growth and yield.

show abstract

An Effort of Mutation Breeding by Oryzalin and Gamma Rays on Wild Raspberry (Rubus sp.) in Cibodas Botanical Garden

Cited by 4 publications

References 6 publications

Stevia Local Tawangmangu Generation M1 Result of Oryzalin Treatment

Stevia Local Tawangmangu Generation M1 Result of Oryzalin Treatment

Induction of Protocorm-Like Bodies (PLBs) Phalaenopsis spp. Hybrids Mutation through Ultraviolet Irradiation (UV254) and Ethyl Methane Sulfonate (EMS)

Effect of Seed Maturity and Storage Duration on Germination of Sambiloto (Andrographis paniculata (Burm.f.) Nees)

Contact Info

Product

Resources

About