An Efficient Transformation Method to Regenerate a High Number of Transgenic Plants Using a New Embryogenic Line of Medicago truncatula cv. Jemalong

Araújo, Susana de Sousa; Duque, Ana Sofia; Santos, Dulce M.; Fevereiro, Pedro

doi:10.1023/b:ticu.0000022540.98231.f8

Cited by 67 publications

(37 citation statements)

References 24 publications

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“…cv. Jemalong line M9-10a seeds (Neves et al 1999;Santos and Fevereiro 2002; Laboratory of Plant Cell Biotechnology, ITQB/UNL, Oeiras, Portugal) were scarified, sterilised and germinated according to Araújo et al (2004). For water-deficit treatments, seedlings were transferred and grown as described in Nunes et al (2008).…”

Section: Methodsmentioning

confidence: 99%

miR398 and miR408 are up-regulated in response to water deficit in Medicago truncatula

Trindade

Capitão

Dalmay

et al. 2009

Planta

368

211

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Plant microRNAs have been implicated in various abiotic stress responses. We identified several conserved microRNAs that showed differential expression in Medicago truncatula plants subjected to water deficit: miR169 is down-regulated only in the roots and miR398a/b and miR408 are strongly up-regulated in both shoots and roots. Down-regulation of miR169 in the roots did not correlate with accumulation of its target MtHAP2-1 transcripts, suggesting that its regulation may not occur at the mRNA level or may depend on other regulatory mechanisms, which do not involve this miRNA, in water-deficit conditions. The up-regulation of miR398a/b and miR408 and the clear down-regulation of their respective target genes, which encode the copper proteins COX5b (subunit 5b of mitochondrial cytochrome c oxidase) and plantacyanin, highlight the involvement of these miRNAs in response to water deprivation in M. truncatula. Also, miR398 up-regulation is inversely correlated with the down-regulation of copper superoxide dismutase, CSD1, during water deficit. The regulation of genes encoding copper proteins by miR398a/b and miR408 suggests a link between copper homeostasis and M. truncatula adaptation to progressive water deficit.

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Section: Methodsmentioning

confidence: 99%

miR398 and miR408 are up-regulated in response to water deficit in Medicago truncatula

Trindade

Capitão

Dalmay

et al. 2009

Planta

368

211

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“…These are Jemalong 2HA (Nolan et al 1989;Rose et al 1999), R108 (Hoffmann et al 1997) and M9--10a (Araújo et al 2004). This suggests that the regeneration process has consistently selected for a somatic cell with a somatic embryogenesis capacity, which is then inherited.…”

Section: Somatic Embryogenesismentioning

confidence: 96%

Medicago truncatula as a model for understanding plant interactions with other organisms, plant development and stress biology: past, present and future

Rose¹

2008

Functional Plant Biol.

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Abstract. Medicago truncatula Gaertn. cv. Jemalong, a pasture species used in Australian agriculture, was first proposed as a model legume in 1990. Since that time M. truncatula, along with Lotus japonicus (Regal) Larsen, has contributed to major advances in understanding rhizobia Nod factor perception and the signalling pathway involved in nodule formation. Research using M. truncatula as a model has expanded beyond nodulation and the allied mycorrhizal research to investigate interactions with insect pests, plant pathogens and nematodes. In addition to biotic stresses the genetic mechanisms to ameliorate abiotic stresses such as salinity and drought are being investigated. Furthermore, M. truncatula is being used to increase understanding of plant development and cellular differentiation, with nodule differentiation providing a different perspective to organogenesis and meristem biology. This legume plant represents one of the major evolutionary success stories of plant adaptation to its environment, and it is particularly in understanding the capacity to integrate biotic and abiotic plant responses with plant growth and development that M. truncatula has an important role to play. The expanding genomic and genetic toolkit available with M. truncatula provides many opportunities for integrative biological research with a plant which is both a model for functional genomics and important in agricultural sustainability.

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“…Thomas et al (1992) and Rose et al (1999) selected and used genotype 2HA, which belongs to M. truncatula ssp. truncatula, the same as genotype M9-10a used by several authors (Santos and Fevereiro 2002;Araujo et al 2004;Confalonieri et al 2010), while Trinh et al (1998) worked with genotype R108, sometimes assigned to M. truncatula ssp. tricycla (Chabaud et al 2007).…”

Section: Barrel Medicmentioning

confidence: 99%

Gene Transfer in Legumes

Atif¹,

Patat-Ochatt²,

Švábová

et al. 2012

Progress in Botany

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An Efficient Transformation Method to Regenerate a High Number of Transgenic Plants Using a New Embryogenic Line of Medicago truncatula cv. Jemalong

Cited by 67 publications

References 24 publications

miR398 and miR408 are up-regulated in response to water deficit in Medicago truncatula

miR398 and miR408 are up-regulated in response to water deficit in Medicago truncatula

Medicago truncatula as a model for understanding plant interactions with other organisms, plant development and stress biology: past, present and future

Gene Transfer in Legumes

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