An efficient regeneration pathway through adventitious organogenesis for the endangered Argania spinosa (L.) Skeels

Amghar, Ilham; Diria, Ghizlane; Boumlik, Imane; Gaboun, Fatima; Iraqi, Driss; Labhilili, Mustapha; Mentag, Rachid; Meziani, Reda; Mazri, Mouaad Amine; Ibriz, Mohammed; Abdelwahd, Rabha

doi:10.1007/s42535-021-00208-y

Cited by 12 publications

(12 citation statements)

References 39 publications

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“…A solid MS medium combined with BA, IAA, and GA 3 was successful in shoot proliferation of E. alpinum [8]. Moreover, the highest adventitious shoots of the endangered plant, A. spinosa, were observed on MS medium containing 1 mg L −1 BA and 2 mg L −1 GA 3 [59].…”

Section: In Vitro Propagationmentioning

confidence: 88%

Genetic Stability, Phenolic, Flavonoid, Ferulic Acid Contents, and Antioxidant Activity of Micropropagated Lycium schweinfurthii Plants

Mamdouh

Mahgoub

Gabr

et al. 2021

Plants

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Lycium schweinfurthii is a Mediterranean wild shrub rich in plant secondary metabolites. In vitro propagation of this plant may support the production of valuable dietary supplements for humanity, introduction of it to the world market, and opportunities for further studies. The presented study aimed to introduce an efficient and reproducible protocol for in vitro micropropagation of L. schweinfurthii and assess the genetic stability of micropropagated plants (MiPs) as well as to estimate phenolic, flavonoid, ferulic acid contents, and the antioxidant activity in leaves of micropropagated plants. Two DNA-based techniques, random amplified polymorphic DNA (RAPD) and inter-simple sequence repeats (ISSR), and one biochemical technique, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), were used to assess the genetic stability in MiPs. Spectrophotometric analysis was performed to estimate total phenolic and flavonoid contents and antioxidant activity of MiPs leaves, while ferulic acid content was estimated using high-performance thin-layer chromatography (HPTLC). Sufficient shoot proliferation was achieved at MS (Murashige and Skoog) medium supplemented with 0.4 mg L−1 kinetin and rooted successfully on half-strength MS medium fortified with 0.4 mg L−1 Indole-3-butyric acid (IBA). The Jaccard’s similarity coefficients detected in MiPs reached 52%, 55%, and 82% in the RAPD, ISSR, and SDS-PAGE analyses, respectively. In the dried leaves of MiPs, the phenolic, flavonoid, and ferulic acid contents of 11.53 mg gallic acid equivalent, 12.99 mg catechin equivalent, and 45.52 mg were estimated per gram, respectively. However, an IC50 of 0.43, and 1.99 mg mL−1 of MiP dried leaves’ methanolic extract was required to scavenge half of the DPPH, and ABTS free radicals, respectively. The study presented a successful protocol for in vitro propagation of a valued promising plant source of phenolic compounds.

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Section: In Vitro Propagationmentioning

confidence: 88%

Genetic Stability, Phenolic, Flavonoid, Ferulic Acid Contents, and Antioxidant Activity of Micropropagated Lycium schweinfurthii Plants

Mamdouh

Mahgoub

Gabr

et al. 2021

Plants

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“…The development of healthy and vigorous roots is essential for successful acclimatization to ex vitro conditions. In a previous study by our group [24], adventitious shoot rooting was achieved on media containing both IBA (1.5 mg L −1 ) and putrescine (160 mg L −1 ). The beneficial effects of polyamines in general and putrescine in particular on rooting were reported in many other plant species, such as olive [30,33], hazelnut [34], almond [35] and teak [36].…”

Section: Discussionmentioning

confidence: 99%

“…The seeds were surface-sterilized by immersion in 70% ethanol for 2 min, followed by immersion in 30% sodium hypochlorite solution containing a few drops of Tween-20 for 15 min. The seeds were then immersed in 0.1% mercury chloride for 10 min, followed by 3 rinses with sterile distilled water, then by immersion in 0.2% polyvinylpyrrolidone solution for 60 min [24]. To promote germination, the seeds were soaked in 10 mg L −1 GA 3 for 12 h and then cultured for 35 days on sucrose-free 1/2MS medium modified by omitting ammonium nitrate [24].…”

Section: Origin Of Plant Materialsmentioning

confidence: 99%

“…The seeds were then immersed in 0.1% mercury chloride for 10 min, followed by 3 rinses with sterile distilled water, then by immersion in 0.2% polyvinylpyrrolidone solution for 60 min [24]. To promote germination, the seeds were soaked in 10 mg L −1 GA 3 for 12 h and then cultured for 35 days on sucrose-free 1/2MS medium modified by omitting ammonium nitrate [24].…”

Section: Origin Of Plant Materialsmentioning

confidence: 99%

“…Developing efficient regeneration systems through somatic embryogenesis or organogenesis will undoubtedly contribute to the propagation, genetic improvement and preservation of argan, as was the case in other oil crops [20][21][22][23]. Recently, a regeneration system has been established for argan through organogenesis [24]. The development of an efficient and reproducible organogenesis system strongly depends on successful in vitro rooting of the regenerated shoots and high survival rates during acclimatization to ex vitro conditions.…”

Section: Introductionmentioning

confidence: 99%

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In Vitro Root Induction from Argan (Argania spinosa (L.) Skeels) Adventitious Shoots: Influence of Ammonium Nitrate, Auxins, Silver Nitrate and Putrescine, and Evaluation of Plantlet Acclimatization

Amghar

Ibriz

Ibrahimi

et al. 2021

Plants

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Argania spinosa (L.) Skeels is an endangered plant species endemic to Morocco. In recent years, attempts to develop in vitro regeneration systems for this species were made. However, rooting and acclimatization of in vitro plants have been a bottleneck for successful propagation. In the present study, the effects of different concentrations of auxins, putrescine, silver nitrate (AgNO3) and ammonium nitrate on the in vitro rooting of adventitious shoots of two argan genotypes “Mejji” and “R’zwa”, were evaluated. The highest rooting percentages (86.6% in “Mejji” and 84.4% in “R’zwa”) were observed on Murashige and Skoog (MS) medium modified by reducing the ammonium nitrate concentration and supplemented with 1.5 mg L−1 indole-3-butyric acid (IBA), 0.5 mg L−1 1-naphthalene acetic acid (NAA), 2 mg L−1 AgNO3 and 160 mg L−1 putrescine. This medium resulted in the development of a good root system after only 10 days of culture. Plantlet acclimatization was carried out using different substrate mixtures, and high survival rates (100%) were observed when the substrate contained either peat alone or a sand–peat mixture (1:1, w/w). The high percentages of rooting and acclimatization reported in the present study are of high importance for rapid and large-scale propagation of this endangered species.

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In Vitro Responses of Some Mediterranean Fruit Crops to Auxin, Cytokinin and Gibberellin Treatments

Mazri

Koufan

Abdelwahd

et al. 2022

Auxins, Cytokinins and Gibberellins Signaling in Plants

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An efficient regeneration pathway through adventitious organogenesis for the endangered Argania spinosa (L.) Skeels

Cited by 12 publications

References 39 publications

Genetic Stability, Phenolic, Flavonoid, Ferulic Acid Contents, and Antioxidant Activity of Micropropagated Lycium schweinfurthii Plants

Genetic Stability, Phenolic, Flavonoid, Ferulic Acid Contents, and Antioxidant Activity of Micropropagated Lycium schweinfurthii Plants

In Vitro Root Induction from Argan (Argania spinosa (L.) Skeels) Adventitious Shoots: Influence of Ammonium Nitrate, Auxins, Silver Nitrate and Putrescine, and Evaluation of Plantlet Acclimatization

In Vitro Responses of Some Mediterranean Fruit Crops to Auxin, Cytokinin and Gibberellin Treatments

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