An efficient protocol for regenerating shoots from paper mulberry (Broussonetia papyrifera) leaf explants

Cui, Siming; Ren, Ying; Hao, Yahan; Zhang, Junjie; Chen, Zhouchao; Zou, Jintuo; Zhou, Wei; Chen, Xiaoyang

doi:10.1515/biol-2020-0034

Cited by 4 publications

(1 citation statement)

References 40 publications

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“…The root biomass of B. papyrifera is large, so the root is an excellent propagation material. Plant tissue culture of B. papyrifera has been reported [ 24 ], but it requires operation under sterile conditions with advanced technical requirements [ 25 ]. However, propagation through root cuttings does not require a sterile environment so it is a convenient method of operation.…”

Section: Introductionmentioning

confidence: 99%

An Efficient Propagation System through Root Cuttings of an Ecological and Economic Value Plant—Broussonetia papyrifera (L.) L’Hér. ex Vent

Zou

Lin

Zhang

et al. 2022

Plants

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Broussonetia papyrifera (L.) L’Hér. ex Vent. has considerable economic and ecological value and a long history of use in China. In this paper, root cuttings were used as the material to establish an efficient vegetative propagation of B. papyrifera. The results revealed that root segments with a diameter of 1.5~2.0 cm and a length of 20~30 cm were most suitable for shoot regeneration, as these segments had the highest adventitious shoot induction rates (93.3%), strongest adventitious shoots, and highest multiplication coefficients (7.07). With regard to the methods used for root burial, a horizontal burial at a depth of 1~3 cm yielded the best results, in this case, the adventitious shoot induction rate can reach 86.7%. The best substrate combination was perlite: peat: coconut chaff = 1:1:1 (v/v/v), wherein the adventitious shoot induction rate can reach 75.6%. The best sterilization method was mixing soil with carbendazim and soaking the root sections in carbendazim for 30 min, wherein the adventitious shoot induction rate can reach 77.8%. Adding 0.2 mg/L naphthaleneacetic acid (NAA) to 1/4 Hoagland nutrient solution significantly improved the rooting rate of adventitious shoots to 82.2%, and the survival rate of the acclimatized plants was more than 90.0%.

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Section: Introductionmentioning

confidence: 99%

An Efficient Propagation System through Root Cuttings of an Ecological and Economic Value Plant—Broussonetia papyrifera (L.) L’Hér. ex Vent

Zou

Lin

Zhang

et al. 2022

Plants

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An efficient in vitro propagation protocol for direct organogenesis from root explants of a multi-purpose plant, Broussonetia papyrifera (L.) L’Hér. ex Vent.

Lin

Zou

Zhang

et al. 2021

Industrial Crops and Products

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In Vitro Micropropagation of Endangered Achillea fragrantissima Forssk. Combined with Enhancement of Its Antihyperglycemic Activity

et al. 2023

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Achillea fragrantissima Forssk. (Family: Asteraceae) has been used as a natural remedy in the Arabian region for its antihyperglycemic activity. As a result of the intensive demand for this plant in folk medicinal uses, its scarcity has become problematic. This study has explored methods that produce an efficient in vitro culture protocol for the conservation of this plant as well as the enhancement of its hypoglycemic activity. A. fragrantissima cultures on Murashige and Skoog (MS) medium supplemented with 3.6 µM/L of 6-benzyl aminopurine (BAP) for a two month period resulted in maximum in vitro shoot proliferation (12.33 shoots/explant) while MS medium supplemented with 2.4 µM/L 1-naphthalene acetic acid (NAA) provided maximum in vitro adventitious root formation (2.46 roots/shoot tip explant). Callus induction was favored by leaf explants cultured on MS medium and supplemented with 3 µM/L BAP and 3 µM/L IAA media in dark conditions. Further in vivo study of some selected feedings determined that the best hypoglycemic activity was obtained in either indole-3-butyric acid (IBA)-fed plants (24%) or NAA-fed plants (22%). Both treatments enhanced insulin-like activity in STZ-treated diabetic Sprague-Dawley rats when compared with the wild plant (10%). Moreover, the IBA-fed plants showed significant antioxidant activity while the NAA-fed plants inhibited salivary alpha amylase. The framework of this study provides in vitro culture methods that can sustain the cultivation of this over-exploited A. fragrantissima plant as well as increase its antioxidant and insulin-like activities.

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An efficient protocol for regenerating shoots from paper mulberry (Broussonetia papyrifera) leaf explants

Cited by 4 publications

References 40 publications

An Efficient Propagation System through Root Cuttings of an Ecological and Economic Value Plant—Broussonetia papyrifera (L.) L’Hér. ex Vent

An Efficient Propagation System through Root Cuttings of an Ecological and Economic Value Plant—Broussonetia papyrifera (L.) L’Hér. ex Vent

An efficient in vitro propagation protocol for direct organogenesis from root explants of a multi-purpose plant, Broussonetia papyrifera (L.) L’Hér. ex Vent.

In Vitro Micropropagation of Endangered Achillea fragrantissima Forssk. Combined with Enhancement of Its Antihyperglycemic Activity

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