An efficient in vitro propagation protocol for direct organogenesis from root explants of a multi-purpose plant, Broussonetia papyrifera (L.) L’Hér. ex Vent.

Lin, Jiana; Zou, Jintuo; Zhang, Bingnan; Que, Qingmin; Zhang, Junjie; Chen, Xiaoyang; Zhou, Wei

doi:10.1016/j.indcrop.2021.113686

Cited by 10 publications

(7 citation statements)

References 42 publications

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“…A study by Zahid et al [ 46 ] showed that the medium had a significant effect on the regeneration efficiency of rhizome explants of Zingiber officinale Roscoe. The high concentration of inorganic salts such as nitrogen and potassium contained in MS medium can meet the nutrient conditions required for rapid growth and promote the growth and long-term survival of the culture, while B5 medium contains low concentrations of ammonium and high concentrations of nitrate, thiamine hydrochloride [ 47 ].…”

Section: Discussionmentioning

confidence: 99%

Efficient In Vitro Sterilization and Propagation from Stem Segment Explants of Cnidoscolus aconitifolius (Mill.) I.M. Johnst, a Multipurpose Woody Plant

Jiang

et al. 2022

Plants

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Cnidoscolus aconitifolius (Mill.) I.M. Johnst is a multipurpose woody plant. In this study, an in vitro efficient propagation system of stem segment explants derived from field-grown C. aconitifolius plants was established for the first time. The sterilization effect, axillary bud initiation, and proliferation efficiency of stem segments were evaluated. The results showed that the sterilization time of 0.1% mercuric chloride, the concentration of Plant Preservative Mixture (PPM), the pretreatment method, and the sampling season had significant effects on the sterilization of stem segments (p < 0.05). The type of medium and plant growth regulators (PGRs) affected the initiation of axillary buds, and the proliferation efficiency was significantly affected by PGRs. The results showed that the best sterilization method for stem segment explants was as follows: a pretreatment by rinsing with running water for 120 min, soaking in 75% ethanol for 50 s, soaking in 0.1% mercuric chloride for 10 min, and medium supplemented with 3 mL/L PPM. When inoculated on the medium in spring, the contamination rate was as low as 25.56%. The optimal initiation medium for axillary buds in stem segments was half-strength Murashige and Skoog (1/2 MS) medium supplemented with 0.5 mg/L 6-benzyladenine (6-BA). The induction rate was as high as 93.33%, and the mean length of axillary buds was 2.47 cm. The optimal proliferation medium was 1/2 MS medium supplemented with 4.0 mg/L 6-BA and 0.2 mg/L indole-3-butyric acid (IBA). The induction rate was up to 80.00%, the total proliferation coefficient was 4.56, and the net proliferation coefficient was 5.69. The 1/2 MS medium supplemented with 0.1 mg/L 6-BA and 1.5 mg/L indole-3-acetic acid (IAA) was most conducive to the elongation of the adventitious shoot, and the adventitious shoot of approximately 1 cm reached 1.93 cm after culturing for 14 days. The best medium for adventitious shoot rooting was 1/2 MS medium supplemented with 0.1 mg/L α-naphthalene acetic acid (NAA), the highest rooting rate was 82.00%, and the survival rate of transplanting was over 90%.

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Section: Discussionmentioning

confidence: 99%

Efficient In Vitro Sterilization and Propagation from Stem Segment Explants of Cnidoscolus aconitifolius (Mill.) I.M. Johnst, a Multipurpose Woody Plant

Jiang

et al. 2022

Plants

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“…At the same time, it was also reported that adventitious shoots were induced by tissue culture with the root segment as the explant. The induction rate can reach 88.9%, and the total multiplication coefficient can reach 5.02 [ 24 ]. Compared with the root cuttings in this experiment, tissue culture can be expanded with less root material.…”

Section: Discussionmentioning

confidence: 99%

“…The root biomass of B. papyrifera is large, so the root is an excellent propagation material. Plant tissue culture of B. papyrifera has been reported [ 24 ], but it requires operation under sterile conditions with advanced technical requirements [ 25 ]. However, propagation through root cuttings does not require a sterile environment so it is a convenient method of operation.…”

Section: Introductionmentioning

confidence: 99%

An Efficient Propagation System through Root Cuttings of an Ecological and Economic Value Plant—Broussonetia papyrifera (L.) L’Hér. ex Vent

Zou

Lin

Zhang

et al. 2022

Plants

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Broussonetia papyrifera (L.) L’Hér. ex Vent. has considerable economic and ecological value and a long history of use in China. In this paper, root cuttings were used as the material to establish an efficient vegetative propagation of B. papyrifera. The results revealed that root segments with a diameter of 1.5~2.0 cm and a length of 20~30 cm were most suitable for shoot regeneration, as these segments had the highest adventitious shoot induction rates (93.3%), strongest adventitious shoots, and highest multiplication coefficients (7.07). With regard to the methods used for root burial, a horizontal burial at a depth of 1~3 cm yielded the best results, in this case, the adventitious shoot induction rate can reach 86.7%. The best substrate combination was perlite: peat: coconut chaff = 1:1:1 (v/v/v), wherein the adventitious shoot induction rate can reach 75.6%. The best sterilization method was mixing soil with carbendazim and soaking the root sections in carbendazim for 30 min, wherein the adventitious shoot induction rate can reach 77.8%. Adding 0.2 mg/L naphthaleneacetic acid (NAA) to 1/4 Hoagland nutrient solution significantly improved the rooting rate of adventitious shoots to 82.2%, and the survival rate of the acclimatized plants was more than 90.0%.

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“…Recently, it was reported that many medicinal plants such as Broussonetia papyrifera L'Hér. Ex Vent (Lin et al, 2021), Eupatorium glandulosum L. (Nithya and Kamalam, 2021) and Mondia whitei (Hook. F.) Skeels (Patricia et al, 2021) produced by tissue culture.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Shoot Regeneration of Lysimachia nummularia L. in Solid and Liquid Culture Medium

Doğan¹

2022

Current Perspectives on Medicinal and Aromatic Plants (CUPMAP)

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Lysimachia nummularia L. is a perennial medicinal and aromatic plant. In this study, the effects of solid and liquid culture media, and plant growth regulators on micropropagation of L. nummularia were investigated. Nodes were used as an explant. Different combinations of thidiazuron (TDZ: 0.05-0.80 mg L -1 ) and indole-3-butyric acid (IBA: 0.10 mg L -1 ) were used as growth regulators in Murashige and Skoog (MS) basal medium. In solid and liquid media experiments, the highest number of shoots was obtained from MS nutrient media supplemented with 0.40 mg L -1 TDZ + 0.10 mg L -1 IBA. The shoot length was higher in both liquid and solid culture medium supplemented with 0.05 mg L -1 TDZ + 0.10 mg L -1 IBA. The best results for number of shoots and regeneration ratio were determined in the solid nutrient media according to results . On the other hand, the best results for shoot length were found in the liquid culture media. Increasing doses of TDZ adversely affected shoot length in both culture media. The regenerated plants were successfully acclimatized to aquatic conditions. The results of this study may help the large-scale propagation of L. nummularia by tissue culture.

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An efficient in vitro propagation protocol for direct organogenesis from root explants of a multi-purpose plant, Broussonetia papyrifera (L.) L’Hér. ex Vent.

Cited by 10 publications

References 42 publications

Efficient In Vitro Sterilization and Propagation from Stem Segment Explants of Cnidoscolus aconitifolius (Mill.) I.M. Johnst, a Multipurpose Woody Plant

Efficient In Vitro Sterilization and Propagation from Stem Segment Explants of Cnidoscolus aconitifolius (Mill.) I.M. Johnst, a Multipurpose Woody Plant

An Efficient Propagation System through Root Cuttings of an Ecological and Economic Value Plant—Broussonetia papyrifera (L.) L’Hér. ex Vent

In Vitro Shoot Regeneration of Lysimachia nummularia L. in Solid and Liquid Culture Medium

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