In Vitro Shoot Regeneration of Lysimachia nummularia L. in Solid and Liquid Culture Medium

Doğan, Muhammet

doi:10.38093/cupmap.1057290

Cited by 2 publications

(2 citation statements)

References 21 publications

(11 reference statements)

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“…Thus, in the propagation of L. nummularia, M. Dogan uses both solid and liquid NM, but the best results regarding the number of shoots and percentage of regeneration were found in solid NM according to the values of in vitro propagation. On the other hand, results regarding the best shoot length formation were noted in liquid NM [20].…”

Section: Resultsmentioning

confidence: 95%

Morphogenesis of cell lines of plants Lysimachia nummularia L. at in vitro culture promising for the content of biologically active substances

Білоус¹,

Matashuk²,

Marchuk³

et al. 2022

Ukr. j. for. wood sci.

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Lysimachia nummularia L. is a plant belonging to the Primulaceae family, which is particularly valuable as a medicinal raw material used in folk medicine in many countries. It has excellent antibacterial and antioxidant capacity of metabolites. That is why the microclonal propagation of Lysimachia nummularia L. is a relevant issue. The purpose of this paper is to develop approaches to microclonal propagation of L. nummularia. For microclonal reproduction of L. nummularia, the method of activation of isolated tissues and organs already present in the plant meristem and induction of direct regeneration directly by explant tissues was used. To select highly productive cell lines of representatives of the genus Lysimachia, callus culture was obtained by indirect morphogenesis from stem and leaf explants. It was found that the formation of tissues and organs of L. nummularia into in vitro culture depended on the composition of the nutrient medium and the quantitative and qualitative ratio of growth regulators in it. Active proliferation of L. nummularia microshoots into in vitro culture was noted on the variants of Murashige and Skoog, and Driver and Kuniyuki nutrient medium with 6-benzylaminopurine 4.0 mg∙l -1, indolyl butyric acid 0.03 mg∙l -1, gibberellic acid 0.1 mg∙l -1. It was established that for microclonal reproduction, induction, and proliferation of the root system and obtaining regenerating plants of L. nummularia, the most effective is the use of nutrient media according to Murashige and Skoog with the addition of thidiazuron 0.5 mg∙l -1 and 0.25 mg∙l -1 kinetin. The optimal conditions for the induction of callusogenesis and obtaining the culture of cells and callus tissues of L. nummularia and its passage in vitro were selected. It has been shown that the modified nutrient medium of Murashige and Skoog, with 2.4-dichlorophenacetic acid 1.5 mg∙l -1 and indole-3-acetic acid 0.2 mg∙l -1, is optimal for the accumulation of callus tissue biomass of L. nummularia, which ensured the frequency of callusogenesis for the first and second passages up to 98.0 ± 0.2%. 5 cell lines that actively synthesize stilbenoids and the highly productive LN-EE 02/19 cell line, which is capable of synthesizing and accumulating in callus tissues up to 10-12 mg∙g-1 of myricetrin, were selectively isolated. As a result of the analysis, the callus culture cell line LN-EE 02/19 was obtained, which allows obtaining myricetrin in amounts up to 10.0-12.0 mg∙l -1 of raw biomass. The developed protocol can be used both for L. nummularia plants and other representatives of the Primulaceae family

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Section: Resultsmentioning

confidence: 95%

Morphogenesis of cell lines of plants Lysimachia nummularia L. at in vitro culture promising for the content of biologically active substances

Білоус¹,

Matashuk²,

Marchuk³

et al. 2022

Ukr. j. for. wood sci.

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“…Furthermore, stem cuttings cannot be used for reproduction of economically important new mulberry varieties (Kapur et al, 2001). However, the plants can be propagated quickly by in vitro culture methods (Hepaksoy, 2017;Zainal & Hepaksoy, 2018;Taha et al, 2020;Doğan, 2022). Propagation of black mulberry via conventional propagation techniques is not satisfactory.…”

Section: Introductionmentioning

confidence: 99%

Effects of charcoal and physical state of medium on micropropagation of black mulbery (Morus nigra L.)

Sevgin

KHALİD>

ÖZKUL>³

2023

Ege Üniversitesi Ziraat Fakültesi Dergisi

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Objective: The purpose of this research was to investigate the effects of charcoal and physical state of culture medium on growth of Morus nigra. L. Material and Methods: For the study tree mature black mulbery (M. nigra) genotypes A5, T6 and T8 grown in vitro were used as the explant source in this study. To find out the possible effects of charcoal and culture physical state (culture medium) on culture growth used four) Liquid Nas and Read Medium (NRM) + 0.5 gl-1 activated charcoal, Liquid NRM containing no charcoal, Gelled NRM + 0.5 gl-1 activated charcoal, agar-gelled NRM containing no charcoal) medium were used in the study to find out the possible effects of the presence of charcoal and physical state of the culture medium on culture growth. Results: Medium physical state and presence of activated charcoal in the medium greatly affected mean shoot number per cultured explants. Mean shoot numbers obtained per explants varied between 1.1 and 5.8. Medium containing no charcoal produced more shoots compared to medium containing charcoal and agar - gelled medium produced more shoots compared to liquid medium. Conclusion: Agar- The gelled medium with agar gave better results for black mulbery (M. nigra) than the liquid medium.

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In Vitro Shoot Regeneration of Lysimachia nummularia L. in Solid and Liquid Culture Medium

Cited by 2 publications

References 21 publications

Morphogenesis of cell lines of plants Lysimachia nummularia L. at in vitro culture promising for the content of biologically active substances

Morphogenesis of cell lines of plants Lysimachia nummularia L. at in vitro culture promising for the content of biologically active substances

Effects of charcoal and physical state of medium on micropropagation of black mulbery (Morus nigra L.)

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