An efficient protocol for commercial micropropagation of malanga (Colocasia esculenta L. Schott) using temporary immersion

Arano-Avalos, Samantha; Gómez-Meriño, Fernando Carlos; Mancilla-Álvarez, Eucario; Sánchez-Páez, R.; Bello-Bello, Jericó Jabín

doi:10.1016/j.scienta.2019.108998

Cited by 22 publications

(16 citation statements)

References 27 publications

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“…Our results showed a higher photosynthetic pigment content in the SETIS TM bioreactor compared to the semi-solid system, the ebb-and-flow bioreactor, and the TIB. In contrast, Arano-Avalos et al [ 5 ] in taro found an increase in photosynthetic pigment content in the TIB compared to the semi-solid medium. Martínez-Estrada et al [ 6 ] reported an increase in chlorophyll accumulation using the ebb-and-flow bioreactor during the in vitro multiplication of A. andreanum .…”

Section: Discussionmentioning

confidence: 81%

“…The use of TI for cocoyam ( Xanthosoma sagittifolium ) in vitro propagation has been reported by Niemenak et al [ 23 ], demonstrating that the TIB is more efficient for multiplication obtaining 68 shoots during four subcultures. Recently, Arano-Avalos et al [ 5 ] in taro ( C. esculenta ) reported three times the multiplication rate in TIB compared to the semi-solid culture medium.…”

Section: Discussionmentioning

confidence: 99%

“…According to Regueira et al [ 33 ], ventilation in culture vessels has an effect on the anatomy and physiological processes of plants under in vitro conditions. In vitro propagation in SABs with airflow allows gas exchange to improve photosynthetic capacity in plants prior to the acclimatization stage [ 5 ].…”

Section: Discussionmentioning

confidence: 99%

“…This species is propagated both sexually and asexually; sexual propagation is rare and produces seeds with low genetic quality [ 3 ], while traditional asexual propagation by corms is seasonal and can cause the spread of pests and diseases [ 4 ]. In vitro propagation of C. esculenta through semi-automated bioreactors (SABs) is an alternative for the production of high-quality genetic and phytosanitary clones [ 5 ]. SABs are designed for in vitro multiplication of plants by tissue, cell or organ in liquid culture medium with a programmed temporary immersion (TI) [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

“…[ 13 ] and apple ( Malus domestica ) [ 19 ]. Taro micropropagation has been carried out in semi-solid medium [ 20 ] and in a TIB [ 5 ]; however, other bioreactors need to be validated as an alternative for micropropagation. The aim of the present study was to compare the efficiency of the TIB, SETIS™, ebb-and-flow bioreactor, and semi-solid medium culture systems for in vitro multiplication of C. esculenta .…”

Section: Introductionmentioning

confidence: 99%

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Comparison of Different Semi-Automated Bioreactors for In Vitro Propagation of Taro (Colocasia esculenta L. Schott)

Mancilla-Álvarez

Pérez-Sato

Núñez-Pastrana

et al. 2021

Plants

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Taro is important for its nutritional content, medicinal use, and bioethanol production. The aim of the present study was to compare different semi-automated bioreactors (SABs) during in vitro multiplication of C. esculenta. The SABs used were temporary immersion bioreactors (TIBs), SETIS™ bioreactors and ebb-and-flow bioreactors; semi-solid culture medium was used as a control treatment. At 30 d of culture, different developmental variables, determination of chlorophyll, stomatal content, and survival percentage during acclimatization were evaluated. SABs increased the shoot multiplication rate relative to the semi-solid medium; however, the SETIS™ bioreactor showed the highest shoot production, with 36 shoots per explant, and the highest chlorophyll content. The stomatal index was higher in the semi-solid medium compared to the SABs, while the percentage of closed stomata was higher in the SABs than in the semi-solid culture medium. The survival rate during acclimatization showed no differences among the culture systems assessed, obtaining survival rates higher than 99%. In conclusion, the SETIS™ bioreactor showed the highest multiplication rate; however, other bioreactor alternatives are available for semi-automation and cost reduction for micropropagation of C. esculenta.

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Section: Discussionmentioning

confidence: 81%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Comparison of Different Semi-Automated Bioreactors for In Vitro Propagation of Taro (Colocasia esculenta L. Schott)

Mancilla-Álvarez

Pérez-Sato

Núñez-Pastrana

et al. 2021

Plants

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BIT® bioreactor increases in vitro multiplication of quality shoots in sugarcane (Saccharum spp. variety LAICA 04-809)

Orozco-Ortiz¹,

Araya-Mattey²,

Vargas-Solórzano³

et al. 2022

Plant Cell Tiss Organ Cult

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Micropropagation of iraca palm (Carludovica palmata Ruiz y Pav) using a temporary immersion system

Minchala-Buestán,

Hoyos-Sánchez,

Correa-Londoño

2023

In Vitro Cell.Dev.Biol.-Plant

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Carludovica palmata is a Neotropical plant with a promising potential for the agroindustry. It is an important source of fibers used to manufacture handmade goods. Colombia is one of the leading countries in the production of this species; however, it lacks efficient techniques for its propagation. We developed a protocol for massive micropropagation of C. palmata using a temporary immersion bioreactor (TIB) system. Immersion frequency, immersion time, culture medium volume, and explant density were evaluated using a split-split-plot design. The variables evaluated were the number and length of shoots and roots, the number of leaves, and the dry weight of shoots. The performance of three micropropagation systems, TIB, semi-solid medium, and liquid medium, was evaluated using a generalized randomized block design. Murashige and Skoog (MS) culture medium with 1.0 mg L−1 of 6-benzylaminopurine, 0.5 mg L−1 of 1-naphthaleneacetic acid, and 20 g L−1 of sucrose added to it was used. The results obtained show a frequency of 12 h with an immersion time of 1 min improved the length of shoots, roots, and number of leaves, and dry weight. A volume of 20 mL/explant was found to be an adequate condition to increase the number and length of shoots, the number of leaves, and the dry weight. According to the optimization module, the factor levels that will maximize the evaluated variables are a 1-minute immersion of the tissues in the culture medium with a frequency of 12 hours each time, with 175 mL of culture medium per bioreactor and density of 10 explants. In conclusion, TIB was shown to be efficient for massive micropropagation of C. palmata compared with conventional methods.

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An efficient protocol for commercial micropropagation of malanga (Colocasia esculenta L. Schott) using temporary immersion

Cited by 22 publications

References 27 publications

Comparison of Different Semi-Automated Bioreactors for In Vitro Propagation of Taro (Colocasia esculenta L. Schott)

Comparison of Different Semi-Automated Bioreactors for In Vitro Propagation of Taro (Colocasia esculenta L. Schott)

BIT® bioreactor increases in vitro multiplication of quality shoots in sugarcane (Saccharum spp. variety LAICA 04-809)

Micropropagation of iraca palm (Carludovica palmata Ruiz y Pav) using a temporary immersion system

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