An efficient plant regeneration through embryogenic callus formation and direct somatic embryogenesis via immature embryo culture in Ipomoea purpurea and I. tricolor

Ishikuro, Hiroki; Nitasaka, Eiji; Otani, Motoyasu

doi:10.5511/plantbiotechnology.14.0216a

Cited by 2 publications

References 13 publications

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In Vitro Protocols for Ornamental Climbers

Shaheen

Shahzad

2015

Biotechnological Strategies for the Conservation of Medicinal and Ornamental Climbers

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Since the time immemorial, plants and plant parts thereof have been used as medicinal sources to cure various ailments and diseases. Recent era witnessed a great loss of this natural wealth because of its excessive use and overexploitation. Thus, it is the need of an hour to take immediate and possible measures for conserving such a great wealth. With the advent of biotechnological approaches, new vitas and horizons were broadened in every sphere of research including plant biotechnology that boosted during the present century and played a vital role in conserving ornamental plants, thus maintaining the depleting natural wealth under laboratory conditions in a short span of time. In vitro studies of several important ornamental climbers have been carried out by different workers, viz., Gloriosa superba , Ceropegia spp . , Allamanda , Passifl ora spp., etc. The present chapter deals with the detailed account of successful protocols developed through in vitro propagation of such elite germplasms.

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In Vitro Protocols for Ornamental Climbers

Shaheen

Shahzad

2015

Biotechnological Strategies for the Conservation of Medicinal and Ornamental Climbers

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Construction of transgenic <i>Ipomoea obscura</i> that exhibits new reddish leaf and flower colors due to introduction of β-carotene ketolase and hydroxylase genes

Otani

Kitayama

Ishikuro

et al. 2021

Plant Biotechnology

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Ipomoea obscura, small white morning glory, is an ornamental plant belonging to the family Convolvulaceae, and cultivated worldwide. I. obscura generates white petals including a pale-yellow colored star-shaped center (flower vein). Its fully opened flowers were known to accumulate trace amounts of carotenoids such as β-carotene. In the present study, the embryogenic calli of I. obscura, were successfully produced through its immature embryo culture, and co-cultured with Agrobacterium tumefaciens carrying the β-carotene 4,4′-ketolase (crtW) and β-carotene 3,3′-hydroxylase (crtZ) genes for astaxanthin biosynthesis in addition to the isopentenyl diphosphate isomerase (idi) and hygromycin resistance genes. Transgenic plants, in which these four genes were introduced, were regenerated from the infected calli. They generated bronze (reddish green) leaves and novel petals that exhibited a color change from pale-yellow to pale-orange in the starshaped center part. Especially, the color of their withered leaves changed drastically. HPLC-PDA-MS analysis showed that the expanded leaves of a transgenic line (T 0 ) produced astaxanthin (5.2% of total carotenoids), adonirubin (3.9%), canthaxanthin (3.8%), and 3-hydroxyechinenone (3.6%), which indicated that these ketocarotenoids corresponded to 16.5% of the total carotenoids produced there (530 µg g −1 fresh weight). Furthermore, the altered traits of the transgenic plants were found to be inherited to their progenies by self-crossing.

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An efficient plant regeneration through embryogenic callus formation and direct somatic embryogenesis via immature embryo culture in Ipomoea purpurea and I. tricolor

Cited by 2 publications

References 13 publications

In Vitro Protocols for Ornamental Climbers

In Vitro Protocols for Ornamental Climbers

Construction of transgenic <i>Ipomoea obscura</i> that exhibits new reddish leaf and flower colors due to introduction of β-carotene ketolase and hydroxylase genes

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