An efficient micropropagation protocol, chemical components, and hypoglycemic activity for Cremastra appendiculata (D. Don) Makino pseudobulbs

Yang, Ningxian; Wang, Daoping; Gao, Yongxing; Hu, Enming; Yu, Xiaosong; Peng, Sijing; Ji, Junfu; Zhang, Mingsheng

doi:10.1007/s11627-021-10222-z

Cited by 8 publications

(2 citation statements)

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“…They also found that these ingredients are mainly concentrated in the outer layer of the pseudobulbs’ skin, and the content decreases from the outside to the inside 13 – 15 . A recent study also found colchicine in the dried pseudobulbs from both in vitro–raised and field-raised C. appendiculata 16 . Colchicine is an alkaloid extracted from the carmus and seeds of Colchicum autumnale in Liliaceae family 17 , which is often used clinically to treat acute gout attacks and to relieve symptoms, such as redness, swelling, heat, and pain within a few hours.…”

Section: Introductionmentioning

confidence: 76%

A draft genome of the medicinal plant Cremastra appendiculata (D. Don) provides insights into the colchicine biosynthetic pathway

et al. 2022

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Cremastra appendiculata (D. Don) Makino is a rare terrestrial orchid with a high market value as an ornamental and Chinese traditional medicinal herb with a wide range of pharmacological properties. The pseudobulbs of C. appendiculata are one of the primary sources of the famous traditional Chinese medicine “Shancigu”, which has been clinically used for treating many diseases, especially, as the main component to treat gout. The lack of genetic research and genome data restricts the modern development and clinical use of C. appendiculata. Here, we report a 2.3 Gb chromosome-level genome of C. appendiculata. We identify a series of candidates of 35 candidate genes responsible for colchicine biosynthesis, among which O-methyltransferase (OMT) gene exhibits an important role in colchicine biosynthesis. Co-expression analysis reveal purple and green-yellow module have close relationships with pseudobulb parts and comprise most of the colchicine pathway genes. Overall, our genome data and the candidate genes reported here set the foundation to decipher the colchicine biosynthesis pathways in medicinal plants.

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Section: Introductionmentioning

confidence: 76%

A draft genome of the medicinal plant Cremastra appendiculata (D. Don) provides insights into the colchicine biosynthetic pathway

et al. 2022

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“…Successful asymbiotic seed productions of several wild orchids have been reported, including Eulophia alta [10], Bletilla striata [11], Cyrtopodium paludicolum [12], (Spathoglottis plicata [13], Cremastra appendiculata (D. Don) Makino [14], and Paphiopedilum tigrinum [15]. Other essential elements influencing the success of in vitro asymbiotic seed cultivations and protocorm development include macro and micro element compositions, vitamins, sucrose, nitrogen, carbohydrates, and organic nutrients [16][17][18].…”

Section: Introductionmentioning

confidence: 99%

Developing Paraphalaenopsis labukensis (Shim, A. Lamb & C.L. Chan), an Orchid Endemic to Sabah, Borneo, Asymbiotic Seed Germination and In Vitro Seedling Development

et al. 2023

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Paraphalaenopsis labukensis Shim, A. Lamb & C.L. Chan (P. labukensis) is an endangered monopodial epiphytic orchid threatened due to habitat fragmentation and overharvesting. Consequently, this research aimed to achieve in vitro propagation of P. labukensis through asymbiotic seed germination and seedling development. This study focused on identification and optimizing capsule maturity, basal media and nutrient requirements. In addition, after hand pollinating the plant, their capsules’ flowers, length, girth, and colour variations were recorded weekly to ascertain morphological maturity characters of this species. Murashige and Skoog (MS), Knudson C (KC), and Vaccin and Went (VW) basal media were used to determine seed vegetation at 60, 90, and 120 days. Subsequently, for the proliferation and development of protocorms, the best basal media were those supplemented with different organic additives (coconut water (CW) and banana homogenate (BH)) and plant growth regulators (PGRs). As a result, 120 days after pollination (DAP) was chosen as the best capsule age for germination since the capsules had reached maturity. The highest germination rate was attained after 90 days of culture in Knudson C (KC) medium, with 98.78 ± 0.89%, followed by Murashige and Skoog (MS) medium, with 92.80 ± 3.26%. The KC supplemented with 0.5 mg/L NAA media documented a maximum percentage of 17.25 ± 0.96%. The 5 g/L (w/v) BH recorded a higher protocorm proliferation percentage than CW. Additionally, after 150 days of culture (DOC), this medium combination produced the most leaves, averaging four or five. The result of this present study has successfully established an effective in vitro propagation protocol for this species.

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Mycobiont identity and light conditions affect belowground morphology and physiology of a mixotrophic orchid Cremastra variabilis (Orchidaceae)

Yagame,

Figura,

Tanaka

et al. 2024

Mycorrhiza

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An efficient micropropagation protocol, chemical components, and hypoglycemic activity for Cremastra appendiculata (D. Don) Makino pseudobulbs

Cited by 8 publications

References 50 publications

A draft genome of the medicinal plant Cremastra appendiculata (D. Don) provides insights into the colchicine biosynthetic pathway

A draft genome of the medicinal plant Cremastra appendiculata (D. Don) provides insights into the colchicine biosynthetic pathway

Developing Paraphalaenopsis labukensis (Shim, A. Lamb & C.L. Chan), an Orchid Endemic to Sabah, Borneo, Asymbiotic Seed Germination and In Vitro Seedling Development

Mycobiont identity and light conditions affect belowground morphology and physiology of a mixotrophic orchid Cremastra variabilis (Orchidaceae)

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