A study on orchids diversity was carried out in the Sungai Kangkawat, Imbak Canyon Conservation Area (ICCA), Sabah, Malaysia. The study aimed to record species composition and distribution of orchids in the furtherance of orchid conservation objective in the gazetted conservation area. A total of 95 species and 30 genera were collected from South Rim, Kawang, and Nepenthes trails. The collection consists of 91 epiphytes, two terrestrials, and two mycoheterotrophs. More orchids were found in the riverine than inland areas. Our study on the species composition based on the vegetation types shows that the presence of inconspicuous and achlorophyllous, either mycoheterotrophic or leafless epiphytic orchids, are indicators of the undisturbed vegetation types. Though the finding was preliminary and brief, our encounter of endemic and rare species underlines the importance of Sungai Kangkawat forest area as an orchid biodiversity hotspot.
A preliminary survey of the diversity of gingers (Zingiberaceae) was conducted in Kadamaian, Kota Belud from 14th to 19th October, 2019. Wild ginger species is utilized widely as one of the most important material in traditional medicine among indigenous people of Sabah. However, few of these plant species have been studied for their chemical constituents and beneficial properties. In order to investigate the compound composition, the essential oil from Etlingera brevilabrum, Alpinia nieuwenhuizii and Hornstedtia havilandii were screened. The essential oil was obtained from leaves, stems and rhizomes of the plant through hydro-distillation and analysed for their chemical composition through Gas Chromatography-Mass Spectrometry (GC-MS). The result of this study indicated that the chemical constituents of all three parts for all species are similar; all have terpenoids (monoterpene and sesquiterpene), aldehyde, hydrocarbon, ketone and alcohol in the essential oil extracts. GC-MS analyses of the oils led to the identification of 35 compound constituents from the leaves, stems and rhizomes of E. brevilabrum, which is the highest. Meanwhile, A. nieuwenhuizii displayed 34 chemical compositions from all parts (leaf, stem and rhizome) of the plant. H. havilandii showed the lowest number of volatiles from all plant parts (24 compounds). Monoterpene is dominant in all wild ginger studied, except for rhizome of E. brevilabrum. On the contrary, E. brevilabrum showed sesquiterpene as the most abundant compound in its composition. This shows that the volatile oil composition of wild ginger species is extremely variable. This study provides preliminary key chemical information for evaluating the quality of local wild gingers in Kadamaian, Kota Belud, Sabah.
A brief orchid diversity study was conducted from 14th to 19th October 2019 in Kadamaian, located northwest of Kinabalu area, through a scientific geographic expedition. A convenience sampling method was employed with only the flowering individuals collected and preserved as herbarium specimens. A total of 58 species and 32 genera were identified during the field visits to the Mount Nopungguk, Melangkap Noriou, Ulu Sungai Melawa, and basecamp site. The finding comprises of 43 epiphytes, 13 terrestrials, and two mycoheterotrophs. Appendicula congesta, Bulbophyllum disjunctum, Dendrobium kiauense, and Goodyera rostellata are species endemic to Borneo found during the expedition along with Crepidium multiflorum, a hyper-endemic species to Kinabalu area. Several rare species which narrowly distributed to primary forest habitat were discovered, including jewel orchids; Cystorchis variegata var. variegata and Anoectochilus geniculatus, and two mycoheterotrophs; Aphyllorchis pallida and Lecanorchis multiflora var. multiflora.
Paraphalaenopsis labukensis Shim, A. Lamb & C.L. Chan (P. labukensis) is an endangered monopodial epiphytic orchid threatened due to habitat fragmentation and overharvesting. Consequently, this research aimed to achieve in vitro propagation of P. labukensis through asymbiotic seed germination and seedling development. This study focused on identification and optimizing capsule maturity, basal media and nutrient requirements. In addition, after hand pollinating the plant, their capsules’ flowers, length, girth, and colour variations were recorded weekly to ascertain morphological maturity characters of this species. Murashige and Skoog (MS), Knudson C (KC), and Vaccin and Went (VW) basal media were used to determine seed vegetation at 60, 90, and 120 days. Subsequently, for the proliferation and development of protocorms, the best basal media were those supplemented with different organic additives (coconut water (CW) and banana homogenate (BH)) and plant growth regulators (PGRs). As a result, 120 days after pollination (DAP) was chosen as the best capsule age for germination since the capsules had reached maturity. The highest germination rate was attained after 90 days of culture in Knudson C (KC) medium, with 98.78 ± 0.89%, followed by Murashige and Skoog (MS) medium, with 92.80 ± 3.26%. The KC supplemented with 0.5 mg/L NAA media documented a maximum percentage of 17.25 ± 0.96%. The 5 g/L (w/v) BH recorded a higher protocorm proliferation percentage than CW. Additionally, after 150 days of culture (DOC), this medium combination produced the most leaves, averaging four or five. The result of this present study has successfully established an effective in vitro propagation protocol for this species.
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