An efficient biocatalytic system for floxuridine biosynthesis based on Lactobacillus animalis ATCC 35046 immobilized in Sr-alginate

Cappa, Valeria Alejandra; Rivero, Cintia W.; Britos, Claudia N.; Martínez, Luis; Lozano, Mario Enrique; Trelles, Jorge A.

doi:10.1016/j.procbio.2014.03.029

Cited by 23 publications

(19 citation statements)

References 43 publications

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“…Ca‐alginate lost its activity after four reuses, similarly to previous reports 29. Therefore, the reusability of alginate‐bentonite biocatalysts was twofold greater than previously reported values using SrCl 2 as cross‐linking solution 22.…”

Section: Resultssupporting

confidence: 85%

“…The elastic modulus of the bionanocomposite beads was close to 60 kPa. In previous works, an improved immobilization method using Sr‐alginate showed an elastic modulus higher than 20 kPa 22. Therefore, the Young's modulus value obtained for the bionanocomposite beads was by 160 % better than that for the improved condition previously reported 22.…”

Section: Resultsmentioning

confidence: 81%

“…2). This bioprocess enables to reach a twofold higher productivity than that obtained under previously optimized conditions 22.…”

Section: Resultsmentioning

confidence: 84%

“…In previous works, an improved immobilization method using Sr‐alginate showed an elastic modulus higher than 20 kPa 22. Therefore, the Young's modulus value obtained for the bionanocomposite beads was by 160 % better than that for the improved condition previously reported 22. Nanosized clay can act as a reinforcing agent and improve mechanical properties as a result of changes in the matrix structure.…”

Section: Resultsmentioning

confidence: 84%

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Bioproduction of Floxuridine Using Nanostabilized Biocatalysts

et al. 2016

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Bionanocomposites employing natural polysaccharides such as alginate and nanoclays, e.g., bentonite, are a promising alternative to developing stabilized biocatalysts used in the pharmaceutical industry due to their physicochemical properties, biocompatibility, and nontoxicity. Mechanical parameters such as swelling ratio, compressive strength, and fracture frequency were optimized, favoring scale-up. Moreover, storage stability and reusability of the biocatalysts were improved by more than 90 % compared with control conditions. In addition, immobilized lactic acid bacteria were used in the bioprocess scale-up to obtain floxuridine, showing a high productivity per gram of biocatalyst.

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Section: Resultssupporting

confidence: 85%

Section: Resultsmentioning

confidence: 81%

“…2). This bioprocess enables to reach a twofold higher productivity than that obtained under previously optimized conditions 22.…”

Section: Resultsmentioning

confidence: 84%

Section: Resultsmentioning

confidence: 84%

See 2 more Smart Citations

Bioproduction of Floxuridine Using Nanostabilized Biocatalysts

et al. 2016

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“…To carry out these bioprocesses under preparative conditions, microorganism immobilization is required to stabilize biocatalysts. Entrapment techniques are widely used for whole cell immobilization [12], providing bioprocess scale-up feasibility and biocatalyst reusability with high operational stability.…”

Section: Introductionmentioning

confidence: 99%