An Effective Protocol for Micropropagation of Edible Bamboo Species (Bambusa tuldaandMelocanna baccifera) through Nodal Culture

Abstract

High demand for edible bamboo shoots of Bambusa tulda and Melocanna baccifera in many Asian ethnic groups has led to the need for developing intensive bamboo farming. To achieve this, in vitro regeneration of bamboo plantlets is needed due to the long and irregular bamboo flowering cycle and scarcity of bamboo seeds. An effective protocol for plantlets regeneration in B. tulda and M. baccifera from nodal explants following validation of the species using the sequence of trnL-F intergenic spacer region is described. Effective axillary bud breaking was achieved at 3 mg/L of 6-benzylaminopurine (BAP) in MS medium. Importantly, combining 2 mg/L of kinetin (Kn) with 3 mg/L of BAP produced a synergistic effect for shoot multiplication in B. tulda and M. baccifera. Under optimized conditions in half-strength MS medium supplemented with 3 mg/L of indole-3-butyric acid (IBA), 10 mg/L of coumarin, and 3% sucrose, profuse production of dark-brown rhizome in B. tulda and abundant rooting (81.67%, P < 0.05, F = 15.46) for M. baccifera within 30 days were achieved. The established protocol and the validation of the reported species at the molecular level will be of help to stakeholders in edible bamboo trade to conserve gene-pool and increase productivity.

Mass propagation of Bambusa bambos (L.) Voss through in vitro culture

“…Most suitable result on surface sterilization was obtained when nodal segments of B. bambos were surface sterilized with 0.2% HgCl 2 for 7 min which resulted in 92% contamination free explants (Table 1). The present results corroborates with the findings of Kheng and Lee, (2011) in B. ventricosa;Waikhom and Louis, (2014) in B. tulda and Melocanna baccifera; Sharma and Sarma, (2011) in B. balcooa; Jha et al (2013) in Dendrocalamus hamiltonii; Arya and Arya, (2015) in D. asper, D. hamiltonii and Drepenostachyum falcatum. In the present investigation, for in vitro shoot induction MS medium was used.…”

&lt;i&gt;In Vitro&lt;/i&gt; Micropropagation of Himalayan Weeping Bamboo, &lt;i&gt;Drepanostachyum falcatum&lt;/i&gt;

“…In Drepanostachyum falcatum, 7 -9 fold shoot multiplication was achieved on MS medium supplemented with 3.5 mg/l BAP (Table 2, Figure 1(c), Figure 1(d)). These results are supported by earlier reports on in vitro propagation of bamboos, where BAP had invariably been used for shoot multiplication [7] [11]- [13] [18]- [20] [22]- [27].…”

In vitro arrow cane (Gynerium sagitatum Aubl.) multiplication in double phase medium

“…Micropropagation is a clonal propagation technique that allows massive plant multiplication in short periods of time, reduced space and under aseptic conditions (Sinhg et al, 2013;Waikhom and Louis, 2014). Micropropagated plants are cultured from explants, established in closed containers with nutrient media and hormone supply that result in high levels of genetic and phenotypic uniformity.…”