An effective microplate method (Biolog MT2) for screening native lignocellulosic-straw-degrading bacteria

Taha, Mohamed; Kadali, Krishna K.; Al-Hothaly, Khalid A.; Smith, Andrew T.; Ball, Andrew S.; Adetutu, Eric M.

doi:10.1007/s13213-015-1044-y

Cited by 17 publications

(10 citation statements)

References 55 publications

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“…Therefore in this study approach to accurate and effective Fusarium fungicides resistance detection was designed and optimized using MT2 microplates (Biolog TM ). MT2 microplates method has been described as screening and evaluation tool for identification and metabolic characterization of bacteria strains ( Kadali et al, 2012 ; Taha et al, 2015 ). However, MT2 microplates method for fungal sensitivity have never been used before.…”

Section: Discussionmentioning

confidence: 99%

“…MT2 microplates method is traditionally used for bacteria identification and the evaluation of their ability to utilize different carbon substrates ( Kadali et al, 2012 ). This system was also successfully used as a method in fast screening of native lignocellulosic-straw-degrading bacteria ( Taha et al, 2015 ), identifying of bacteria, which are able to decompose hydrocarbon fractions ( Kadali et al, 2012 ) and metabolize microcystin-LR ( Manage et al, 2009 ). However, to the best of our knowledge, there are no reports concerning the use of this technique to determine fungicides resistance of the Fusarium isolates.…”

Section: Introductionmentioning

confidence: 99%

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Fast and Accurate Microplate Method (Biolog MT2) for Detection of Fusarium Fungicides Resistance/Sensitivity

et al. 2016

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The need for finding fungicides against Fusarium is a key step in the chemical plant protection and using appropriate chemical agents. Existing, conventional methods of evaluation of Fusarium isolates resistance to fungicides are costly, time-consuming and potentially environmentally harmful due to usage of high amounts of potentially toxic chemicals. Therefore, the development of fast, accurate and effective detection methods for Fusarium resistance to fungicides is urgently required. MT2 microplates (BiologTM) method is traditionally used for bacteria identification and the evaluation of their ability to utilize different carbon substrates. However, to the best of our knowledge, there is no reports concerning the use of this technical tool to determine fungicides resistance of the Fusarium isolates. For this reason, the objectives of this study are to develop a fast method for Fusarium resistance to fungicides detection and to validate the effectiveness approach between both traditional hole-plate and MT2 microplates assays. In presented study MT2 microplate-based assay was evaluated for potential use as an alternative resistance detection method. This was carried out using three commercially available fungicides, containing following active substances: triazoles (tebuconazole), benzimidazoles (carbendazim) and strobilurins (azoxystrobin), in six concentrations (0, 0.0005, 0.005, 0.05, 0.1, 0.2%), for nine selected Fusarium isolates. In this study, the particular concentrations of each fungicides was loaded into MT2 microplate wells. The wells were inoculated with the Fusarium mycelium suspended in PM4-IF inoculating fluid. Before inoculation the suspension was standardized for each isolates into 75% of transmittance. Traditional hole-plate method was used as a control assay. The fungicides concentrations in control method were the following: 0, 0.0005, 0.005, 0.05, 0.5, 1, 2, 5, 10, 25, and 50%. Strong relationships between MT2 microplate and traditional hole-plate methods were observed regarding to the detection of Fusarium resistance to various fungicides and their concentrations. The tebuconazole was most potent, providing increased efficiency in the growth inhibition of all tested isolates. Almost all among tested isolates were resistant to azoxystrobin-based fungicide. Overall, the MT2 microplates method was effective and timesaving, alternative method for determining Fusarium resistance/sensitivity to fungicides, compering to traditional hole-plate approach.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Fast and Accurate Microplate Method (Biolog MT2) for Detection of Fusarium Fungicides Resistance/Sensitivity

et al. 2016

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“…A Biolog MT2 microplate (Hayward, CA, USA)-based assay was used for the rapid identification and evaluation of the ability of a given bacterial strain to utilize PBDEs as a sole carbon substrate. Each Biolog MT2 microplate well was created as a sole carbon source substrate well using MSB; in addition, an equal concentration of tetrazolium violet dye, which is sensitive to the oxidation of a carbon source and to bacterial respiration, was added (Taha et al 2015 ; Chang et al 2020 ). Different types of PBDEs, namely BDE-209 (25.0 μg L -1 ), BDE-15 (25.0 μg L -1 ), and a mixture of 38 PBDE congeners, including mono-BDE, di-BDE, tri-BDE, tetra-BDE, penta-BDE, hexa-BDE, and hepta-BDE were selected as the carbon sources.…”

Section: Methodsmentioning

confidence: 99%

A coupled UV photolysis-biodegradation process for the treatment of decabrominated diphenyl ethers in an aerobic novel bioslurry reactor

Chang

Chen

Chou

et al. 2020

Environ Sci Pollut Res

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The commercial flame retardant is an emerging contaminant (EC) commonly found in soils and sediments. A coupled UVphotolysis-biodegradation process was used to decompose decabromodiphenyl ether (BDE-209) in clay slurries. A novel bioslurry bioreactor (NBB) was employed in which BDE-209 degradation was maximized by the simultaneous application of LED UVA irradiation and biodegradation by a mixed bacterial culture. The rate of BDE-209 degradation decreased in the order: coupled UV photolysis-biodegradation (1.31 × 10-2 day-1) > UV photolysis alone (1.10 × 10-2 day-1) > biodegradation alone (1.00 × 10-2 day-1). Degradation intermediates detected included hydroxylated polybrominated diphenylethers, partially debrominated PBDE congeners and polybrominated dibenzofuran. The UV-resistant bacterial strains isolated that could utilize BDE-209 as a sole carbon source included Stenotrophomonas sp., Pseudomonas sp., and Microbacterium sp. These strains encoded important functional genes such as dioxygenase and reductive dehalogenases. Continuous UV irradiation during the NBB process affected various biochemical oxidative reactions during PBDEs biodegradation. Simultaneous photolysis and biodegradation in the NBB system described reduces operational time, energy, expense, and maintenance-demands required for the remediation of BDE-209 when compared to sequential UV-biodegradation process or to biodegradation alone. Keywords Coupled UV photolysis-biodegradation. Decabromodiphenyl ether. Novel bioslurry bioreactor. UV-resistant bacterial strains. Polybrominated dibenzofuran Highlights • A time, energy, and cost-saving technique using a coupled UV photolysis-biodegradation process for degrading BDE-209 in a novel bioslurry bioreactor. • Identification of isolated UV-resistant bacterial strains that potentially are able to utilize PBDEs as sole carbon source and the analysis of related functional genes. • The prediction of a possible pathway for BDE-209 degradation by coupled photolysis-biodegradation.

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“…To prepare an inoculum, each isolate was cultivated on Potato Dextrose Agar medium (PDA) (Oxoid Ltd., England) with a 3% addition of oak sawdust (SDM), beet pulp (BPM) or, wheat bran (WBM), and control medium (CLM) without any additives, at 27°C in the dark, for 25 days including 7 days with white light exposure for spore formation. 100 μl of the mycelium water suspension was added to wells on the MT2 plate, where previously 50 μl of 1% SD, BP or WB water solution was placed, following the modified procedures of Kadali et al (2012) , Taha et al (2015) and Frąc et al (2016) , in four replicates. The inoculated microplates were incubated at 27°C for 10 days.…”

Section: Methodsmentioning

confidence: 99%

Metabolic and Genetic Properties of Petriella setifera Precultured on Waste

et al. 2018

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Although fungi that belong to Petriella genus are considered to be favorable agents in the process of microbial decomposition or as plant endophytes, they may simultaneously become plant pests. Hence, nutrition factors are supposed to play an important role. Therefore, it was hypothesized that Petriella setifera compost isolates, precultured on three different waste-based media containing oak sawdust, beet pulp (BP) and wheat bran (WB) will subsequently reveal different metabolic properties and shifts in genetic fingerprinting. In fact, the aim was to measure the influence of selected waste on the properties of P. setifera. The metabolic potential was evaluated by the ability of five P. setifera strains to decompose oak sawdust, BP and WB following the MT2 plate® method and the catabolic abilities of the fungus to utilize the carbon compounds located on filamentous fungi (FF) plates®. Genetic diversity was evaluated using Amplified Fragment Length Polymorphism analysis performed both on DNA sequences and on transcript-derived fragments. P. setifera isolates were found to be more suitable for decomposing waste materials rich in protein, N, P, K and easily accessible sugars (as found in WB and BP), than those rich in lignocellulose (oak sawdust). Surprisingly, among the different waste media, lignocellulose-rich sawdust-based culture chiefly triggered changes in the metabolic and genetic features of P. setifera. Most particularly, it contributed to improvements in the ability of the fungus to utilize waste-substrates in MT2 plate® and two times increase the ability to catabolize carbon compounds located in FF plates®. Expressive metabolic properties resulting from being grown in sawdust-based substrate were in accordance with differing genotype profiles but not transcriptome. Intraspecific differences among P. setifera isolates are described.

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An effective microplate method (Biolog MT2) for screening native lignocellulosic-straw-degrading bacteria

Cited by 17 publications

References 55 publications

Fast and Accurate Microplate Method (Biolog MT2) for Detection of Fusarium Fungicides Resistance/Sensitivity

Fast and Accurate Microplate Method (Biolog MT2) for Detection of Fusarium Fungicides Resistance/Sensitivity

A coupled UV photolysis-biodegradation process for the treatment of decabrominated diphenyl ethers in an aerobic novel bioslurry reactor

Metabolic and Genetic Properties of Petriella setifera Precultured on Waste

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