An economical, upgraded, stabilized and efficient preparation of amyloglucosidase

Shah, Nilesh; Shah, D.N.; Upadhyay, C. M.; Nehete, Pramod N.; Kothari, R. M.; Hegde, Mahabaleshwar V.

doi:10.1016/0168-1656(89)90070-9

Cited by 16 publications

(5 citation statements)

References 13 publications

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“…One unit of a-amylase is defined as the amount of enzyme giving red colour equivalent to 20 mg standard dextrin in 30s at 40°C at pH 7.0 as a result of starch liquefaction. AMG 200-L@ was estimated by incubating it with 2% (w/v) soluble starch (60°C, pH 4.3, 10 min) and determining the reducing sugars formed as discussed earlier [5,6]. One unit of AMG 200-L@ is defined as the amount ouf enzyme releasing 1 mg reducing sugars measured as dextrose per min under assay conditions.…”

Section: Methodsmentioning

confidence: 99%

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Alternate Economical Starchy Substrates for the Production of 70% Sorbitol

Upadhyay¹,

Nehete

Shah³

et al. 1991

Starch Stärke

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In view of the soaring prices of corn and tapioca starch, use of their hydrolysate in the production of 70% sorbitol became less remunerative. Therefore, an economical alternative is explored by using hydrolysates of cereal flours, namely, rice (Oryzae sativa), wheat (Triticum aestivum), jowar (Sorghum vulagare) and bajra (Pennisetum typhoideum). A protocol is devised to a) prepare their high DE hydrolysates, b) purify it after saccharification, c) monitor the chemical characteristics of concentrated hydrolysate, as fedstock for Raney nickel catalyzed pressure hydrogenation and d) finally prepare 70% sorbitol. Merits and demerits of hydrolysates of these cereal flours are discussed in terms of operational limitations and percentage recovery, the governing factors for their industrial acceptability. Rice flour hydrolysate appears to be an alternative substrate, operationally and economically.

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Section: Methodsmentioning

confidence: 99%

“…Aspergillus and Pencillium sp. by adsorption to several ion exchange resins have been reported [ 5 ] and such systems for steroid transformation has been discussed [6] in literature.…”

Section: Introductionmentioning

confidence: 99%

Alternate Economical Starchy Substrates for the Production of 70% Sorbitol

Upadhyay¹,

Nehete

Shah³

et al. 1991

Starch Stärke

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“…It was produced by (a) preparing an aqueous slurry of tapioca starch (32% w/v), (b) liquefying it with Termamyl 120L (0.2 units/g of starch, pH 6.4, 9SoC, 30 min), (c) arresting the liquefaction (pH 4.5, 9SoC, 15min) and (d) saccharifying it with AMG-200L (13 units/g of starch, 55kloC, 55-6011) as described earlier by us [5].…”

Section: Tapioca Starch Hydrolysate (Tsh) Preparationmentioning

confidence: 99%

Isolation of Sorbitol‐6‐Phosphate from Hydrogenated Tapioca Starch Hydrolysate

1996

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“…Although, it is not precisely clear as to which ingredient from the residue stabilized Bamylase activity, it is obvious that clarification removed stabilityimparting ingredient(s). However, in view of high cost of stabilization by refrigeration, we opted for a chemical mode of stabilization at ambient temperature as reported earlier (Shah et al, 1989). Minimal 80% stability afforded by 0.5% (w/v) thiourea for 6 weeks paved the way to stabilize B-amylase at ambient temperature.…”

Section: Stability As a Function Of Stabilizersmentioning

confidence: 99%