An eco-friendly HPTLC method for assay of eszopiclone in pharmaceutical preparation: investigation of its water-induced degradation kinetics

Abstract

An eco-friendly, sensitive and selective high-performance thin-layer chromatographic method was developed and validated for analysis of eszopiclone in bulk powder and formulation. The method employed HPTLC aluminium plates precoated with silica gel 60F-254 as the stationary phase. The solvent system consisted methanol-water (6: 4, v/v). Densitometric analysis of eszopiclone was carried out at 300 nm. This system was found to give compact spot for eszopiclone (R f value of 0.48 ± 0.02). The proposed method was validated for linearity, precision, robustness, LOD, LOQ, specificity and accuracy in accordance with ICH guidelines.Linearity was found to be in the range of 0.2-1.2 µg/band with significantly high value of correlation coefficient r = 0.9997. Statistical comparison with a reported reference method showed similar results with respect to accuracy and precision Study of the eszopiclone water-induced degradation kinetic was carried out by the proposed HPTLC method. Activation energy and degradation rate constant at room temperature were calculated after construction of Arrhenius plot.Zone Block 2, Alexandria, Egypt. All chemicals and reagents used were of analytical grade and were purchased from Merck Chemicals, Egypt. InstrumentationThe samples were applied to the plates using a 100-µL CAMAG microsyringe (Hamilton, Bonaduz, Switzerland) in the form of bands using a CAMAG Linomat IV (Switzerland) applicator. Slit dimension was kept at 6 × 0.2 mm and a 20-mm s -1 scanning speed was employed. Ascending development of the mobile phase was carried out in 20 × 20 cm twin trough glass chamber (CAMAG, Muttenz, Switzerland) and the top of chamber was covered tightly with the lid. The optimized chamber saturation time for mobile phase was 30 min at room temperature (25 °C ± 2). CAMAG TLC Scanner III operated in the reflectance-absorbance mode and controlled by CATS software (V 3.15, CAMAG). The source of radiation utilized was deuterium lamp emitting a continuous ultraviolet (UV) spectrum between 190 and 400 nm. 2.3.Chromatographic conditionsChromatographic studies were performed using the following conditions:Stationary phase: Silica gel 60 F pre coated HPTLC 254 plates (20 × 10 cm, aluminum plates with 250-µm thickness), Prewashing with methanol, drying in oven (60±1ºC, 5 min),

A novel smartphone HPTLC assaying platform versus traditional densitometric method for simultaneous quantification of alfuzosin and solifenacin in their dosage forms as well as monitoring content uniformity and drug residues on the manufacturing equipment

“…Their values were found to surpass 0.9990, a good indication of the peaks' homogeneity as well as the absence of any masked co-eluting components. 47,48 Moreover, the similar identity of the standard and sample of each drug was guaranteed through the good correlation and superimposition of their UV spectra, as illustrated in Fig. 3A and B.…”

Simple simultaneous determination of moxifloxacin and metronidazole in complex biological matrices

“…Chromatographic analysis offers many advantages for the analysis of various compounds. 13–27 Thus, our proposed RP-HPLC method shows many advantages over the reported ones. It shows higher selectivity and sensitivity.…”

Economic Electrochemical Sensors for the Determination of Eszopiclone in Pharmaceutical Formulation with Greenness Profile Assessment

“…In this work, two novel comprehensive approaches were applied for evaluating the greenness of the applied analytical methodology; GAPI and analytical Eco-scale [53][54][55]. These approaches compare different parameters and different steps of our developed analytical method to those applied in previous reports including; HPLC [16], HPTLC [23] and spectrophotometric [7] methods.…”

“…Few methods have been reported for the determination of EZP pharmaceutical dosage forms in literature. Pandya et al [7] has reported a simple spectrophotometric method by direct measurement of maximum absorbance of EZP peak at 305 nm, El-Yazbi et al [23] has reported a HPTLC method for EZP determination using methanolwater (6 : 4, v/v) as a mobile phase. While a HPLC method was reported by Ravi et al [16] with a mobile phase consisting of phosphate buffer of pH, 3.5 and acetonitrile in the ratio of 50 : 50 (v/v).…”

“…Some analytical methods have been reported in literature for EZP determination, these include UV spectrophotometric [5–8], spectrofluorometric [9], HPLC [10–20], UPLC [13, 21, 22] and TLC [9, 23] methods. To the best of our knowledge no potentiometric techniques has been previously published for the determination of EZP.…”