An early humoral immune response in peripheral blood following parenteral inactivated influenza vaccination

Cox, Rebecca Jane; Brokstad, Karl Albert; Zuckerman, Mark; Wood, John M.; Haaheim, Lars R.; Oxford, John

doi:10.1016/0264-410x(94)90334-4

Cited by 139 publications

(131 citation statements)

References 19 publications

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“…In these studies, PCs peaked at around a week following immunization. [17][18][19] The PC response to a booster dose of a glycoconjugate vaccine is more rapid than following a primary dose, similar to the PC kinetics following repeated protein vaccination. The peak in PC responses following booster vaccination with a MenC glycoconjugate in 1-y-old children 5 or 13-to 15-y-old adolescents 7 appeared at day 6, which is more rapid than following the first vaccine dose in infants but slower than that following the third dose of an infant primary series.…”

Section: Plasma Cell Kineticsmentioning

confidence: 90%

Polysaccharide-specific B cell responses to vaccination in humans

Mitchell

Kelly

Pollard

et al. 2014

Human Vaccines & Immunotherapeutics

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Section: Plasma Cell Kineticsmentioning

confidence: 90%

Polysaccharide-specific B cell responses to vaccination in humans

Mitchell

Kelly

Pollard

et al. 2014

Human Vaccines & Immunotherapeutics

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“…Immunological tests. Lymphocytes were isolated from the blood, tonsils and nasal tissue using Lymphoprep (Nycomed Pharma, Oslo, Norway) and were analyzed by the ELISPOT method [5]. Antibodies in the oral fluid and serum were quantified by the ELISA method [6].…”

Section: Methodsmentioning

confidence: 99%

“…All capture and detector Abs were purchased from Sigma (St. Louis, MI, USA). In addition, the level of serum antibody to the viruses was analyzed by the HI test [5].…”

Section: Methodsmentioning

confidence: 99%

High Prevalence of Influenza Specific Antibody Secreting Cells in Nasal Mucosa

Brokstad¹,

Cox

Eriksson

et al. 2001

Scand J Immunol

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Secretory immunoglobulin A (SIgA) provides the first line of defence against pathogens initiating infection via the mucosal route, e.g. the influenza virus. The aim of this study was to examine the basal level of influenza-specific antibody-secreting cell (ASC) in the local mucosa of the upper respiratory tract. Nineteen patients scheduled for tonsillectomy were enrolled for the study, and they had not experienced influenza during the previous year. Tonsils, blood, oral fluid and a nasal biopsy were sampled, and the basal levels of ASC and antibodies (Abs) were determined. We found low numbers of influenza-specific ASC in the blood and tonsils, but there were about 10±100 times higher numbers of specific ASC in the nasal mucosa tissue despite no recent influenza exposure. Thus, the basal level of influenza-specific ASC in the mucosa of the respiratory tract may be important in the protection against influenza infection.

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“…The enzyme-linked immunospot assay is a well-established method for the study of the immune response and the secretion of antibodies from B lymphocytes in both natural infections and vaccine experiments (5,6,9,11,12), although the procedures are laborious and include time-consuming incubation steps. Further development based on this technology has revealed that specific antibodies can be detected after purified lymphocytes were placed in appropriately coated enzymelinked immunosorbent assay (ELISA) wells and kept for 1 to 2 h at 37°C to allow the spontaneous secretion of antibodies, referred to here as the PlasmAcute technology (9).…”

mentioning

confidence: 99%

“…This response can be detected at about day 2 or 3 to day 16 or 17 following vaccination. Plasma immunoglobulin G (IgG) can usually be detected from day 12 onwards in this system (5,6).…”

mentioning

confidence: 99%