An early Ca2+ influx is a prerequisite to thaxtomin A-induced cell death in Arabidopsis thaliana cells

Errakhi, Raffik; Dauphin, Aurélien; Meimoun, Patrice; Lehner, Arnaud; Reboutier, David; Vatsa, Parul; Briand, Joël; Madiona, Karine; Rona, Jean‐Pierre; Barakate, Mustapha; Wendehenne, David; Beaulieu, Carole; Bouteau, François

doi:10.1093/jxb/ern267

Cited by 62 publications

(65 citation statements)

References 54 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Other studies have found JA-responsive genes to be upregulated with the exogenous application of thaxtomin A to A. thaliana cells. It induced an influx of calcium and the expression of PAL-1 but not PR-1 in one case (15), and induced the expression of many defense-related genes in a microarray analysis, including PAL2, POX, a thaumatin family PR protein, and a PIN-related protein (14). These results differ from those we have obtained, suggesting that perhaps thaxtomin, or other bacterial determinants of S. scabies, induce different responses in different plant systems, as seen in a study which demonstrated that thaxtomin A did not induce a similar cellular response in A. thaliana and tobacco, another solanaceous species (26).…”

Section: Discussionmentioning

confidence: 89%

See 1 more Smart Citation

Long-Term Induction of Defense Gene Expression in Potato by Pseudomonas sp. LBUM223 and Streptomyces scabies

Arseneault¹,

Pieterse²,

Gérin-Ouellet³

et al. 2014

Phytopathology®

View full text Add to dashboard Cite

Streptomyces scabies is a causal agent of common scab of potato, which generates necrotic tuber lesions. We have previously demonstrated that inoculation of potato plants with phenazine-1-carboxylic acid (PCA)-producing Pseudomonas sp. LBUM223 could significantly reduce common scab symptoms. In the present study, we investigated whether LBUM223 or an isogenic phzC-mutant not producing PCA could elicit an induced systemic resistance response in potato. The expression of eight defense-related genes (salicylic acid [SA]-related ChtA, PR-1b, PR-2, and PR-5; and jasmonic acid and ethylene-related LOX, PIN2, PAL-2, and ERF3) was quantified using newly developed TaqMan reversetranscription quantitative polymerase chain reaction assays in 5-and 10-week-old potted potato plants. Although only wild-type LBUM223 was capable of significantly reducing common scab symptoms, the presence of both LBUM223 and its PCA-deficient mutant were equally able to upregulate the expression of LOX and PR-5. The presence of S. scabies overexpressed all SA-related genes. This indicates that (i) upregulation of potato defense-related genes by LBUM223 is unlikely to contribute to common scab's control and (ii) LBUM223's capacity to produce PCA is not involved in this upregulation. These results suggest that a direct interaction occurring between S. scabies and PCA-producing LBUM223 is more likely involved in controlling common scab development.

show abstract

Section: Discussionmentioning

confidence: 89%

“…To our knowledge, S. scabies' ability to induce SAR in potato plants has never been studied. However, some reports suggest that S. scabies, or the thaxtomin A it produces, are capable of inducing defense responses in A. thaliana (5,14,15,23).…”

mentioning

confidence: 99%

Long-Term Induction of Defense Gene Expression in Potato by Pseudomonas sp. LBUM223 and Streptomyces scabies

Arseneault¹,

Pieterse²,

Gérin-Ouellet³

et al. 2014

Phytopathology®

View full text Add to dashboard Cite

show abstract

“…11 Cell death appeared to be dose dependent for both suspension cells ( Fig. 1) with a lag of sensitivity in BY2.…”

Section: Two Different Signaling Pathways For Thaxtomin A-induced Celmentioning

confidence: 90%

“…In A. thaliana cells, 10 μM TXT induces a rapid influx of Ca 2+ through the PM inhibited by La 3+ , Gd 3+ or BAPTA. 11 In BY2 cells, also expressing aequorin in their cytosol, no significant increase in [Ca 2+ ] cyt were recorded upon addition of 20 μM TXT (Fig. 2A).…”

Section: Two Different Signaling Pathways For Thaxtomin A-induced Celmentioning

confidence: 91%

“…In A. thaliana cells, the influx of Ca 2+ is an upstream event in the signaling pathway leading to TXT-induced cell death. 11 We thus compared the effect of a 6 hours treatment with TXT on both cell lines in presence or absence of La 3+ , a PM Ca 2+ channel inhibitor. As expected, La 3+ pretreatment of BY2 cells failed to decrease the TXT-induced cell death (Fig.…”

Section: Two Different Signaling Pathways For Thaxtomin A-induced Celmentioning

confidence: 99%

See 1 more Smart Citation

Two different signaling pathways for thaxtomin A-induced cell death in Arabidopsis and tobacco BY2

Meimoun¹,

Tran²,

Baz³

et al. 2009

Plant Signaling & Behavior

Self Cite

View full text Add to dashboard Cite

of defence-related genes. 1,2 Additionally, plants often induce a hypersensitive response (HR) characterized by a localized cell death often associated with disease resistance. 3 This form of programmed cell death (PCD) 4 requires gene expression and metabolic activities 3 that displays apoptosis-like features. Thaxtomin A (TXT) is a nitrated dipeptide phytotoxin produced by all plant-pathogenic of the Streptomyces species. 5,6 TXT inhibits the cellulose synthesis, suggesting that cell wall is one of the main target of TXT. 7,8 In the roots of different species, TXT stimulates H + efflux across plasma membrane (PM) and a short lived Ca 2+ influx, inhibited by La 3+ , a PM Ca 2+ channel inhibitor. 9 TXT also induces cell death depending on active gene transcription and de novo protein synthesis, which are PCD hallmarks. 10 We recently reported new insights regarding the effect of TXT 11 by using A. thaliana suspension cells, a convenient material for studying early physiological events induced by pathogens. 10,[12][13][14][15] We showed that the transient increase in [Ca 2+ ] cyt was a key step of a further signaling pathway leading to the death of the cells in response to a TXT accordingly to previous studies which showed that Ca 2+ activity changes are involved in PCD in plant, as in animal. 16,17 Fast rises in [Ca 2+ ] cyt are effectively also frequently described as one of the earliest responses to various microbial phytotoxins and elicitors 18,19 and numerous studies led to the conclusion that activation of defence responses depends on Ca 2+ influxes from the apoplast into the cytosol of plant cells. 16 Notably, elicitor-induced uptake of Ca 2+ from the extracellular medium was shown to be required for the controlled generation of H 2 O 2 , 20-22 the activation of MAPK pathways, 2,23 the activation of defence related genes 24 and production of phytoalexin. 16 In the present work, we extended our study by comparing the TXT-induced responses in A. thaliana and N. tabacum BY2 suspension cells. The cell death detection experiments and Ca 2+ assay we performed demonstrate that the signal transduction pathways leading to the cell death in response to TXT are different in these two plant models.Arabidopsis thaliana L. suspension cells were grown in Gamborg medium (pH 5.8) and Nicotiana tabacum BY2 suspension cells were grown in Murashige and Skoog medium (pH 5.8). All experiments were performed using log-phase cells (4 days after sub-culture for Arabidopsis and 6 days for BY2). Cell death was quantified using [Plant Signaling & Behavior 4:2, 142-144; February 2009] ; ©2009 Landes BioscienceThaxtomin A (TXT) is a phytotoxin produced by all plantpathogenic Streptomyces scabies involved in the potato scab disease. Their pathogenicity was previously correlated with the production of TXT. Calcium is known to be an essential second messenger associated with pathogen-induced plant responses and cell death. We have effectively shown that in Arabidopsis thaliana cell suspensions, TXT induces an early short lived Ca 2+ inf...

show abstract

The Function of Cyclic Nucleotide-Gated Channels in Biotic Stress

Ma¹,

Yoshioka²,

Gehring³

et al. 2010

Ion Channels and Plant Stress Responses

View full text Add to dashboard Cite

An early Ca2+ influx is a prerequisite to thaxtomin A-induced cell death in Arabidopsis thaliana cells

Cited by 62 publications

References 54 publications

Long-Term Induction of Defense Gene Expression in Potato by Pseudomonas sp. LBUM223 and Streptomyces scabies

Long-Term Induction of Defense Gene Expression in Potato by Pseudomonas sp. LBUM223 and Streptomyces scabies

Two different signaling pathways for thaxtomin A-induced cell death in Arabidopsis and tobacco BY2

The Function of Cyclic Nucleotide-Gated Channels in Biotic Stress

Contact Info

Product

Resources

About