The Function of Cyclic Nucleotide-Gated Channels in Biotic Stress

Ma, Wei; Yoshioka, Keiko; Gehring, Chris; Berkowitz, Gerald A.

doi:10.1007/978-3-642-10494-7_8

Cited by 15 publications

(6 citation statements)

References 77 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Analyses of knock-out lines have revealed that both AtCNGC11 and AtCNGC12 are also positive mediators of resistance against an avirulent biotype of Hyaloperonospora parasitica [ 27 ]. This may suggest that specific cyclic nucleotide signatures generated in response to biotic [ 9 , 28 ] and abiotic [ 29 ] stresses act as messengers in signaling cascades that critically depend on CNGCs [ 30 , 31 ]. Cyclic AMP may also have an important role in abiotic stress responses and in particular responses to NaCl stress since voltage independent channels (VICs) in Arabidopsis thaliana roots have been reported to have open probabilities sensitive to μmolar concentrations cAMP or cGMP at the cytoplasmic side [ 32 ].…”

Section: Is There Camp-dependent Signaling In Plants?mentioning

confidence: 99%

Adenyl cyclases and cAMP in plant signaling - past and present

2010

Self Cite

View full text Add to dashboard Cite

In lower eukaryotes and animals 3'-5'-cyclic adenosine monophosphate (cAMP) and adenyl cyclases (ACs), enzymes that catalyse the formation of cAMP from ATP, have long been established as key components and second messengers in many signaling pathways. In contrast, in plants, both the presence and biological role of cAMP have been a matter of ongoing debate and some controversy. Here we shall focus firstly on the discovery of cellular cAMP in plants and evidence for a role of this second messenger in plant signal transduction. Secondly, we shall review current evidence of plant ACs, analyse aspects of their domain organisations and the biological roles of candidate molecules. In addition, we shall assess different approaches based on search motifs consisting of functionally assigned amino acids in the catalytic centre of annotated and/or experimentally tested nucleotide cyclases that can contribute to the identification of novel candidate molecules with AC activity such as F-box and TIR proteins.

show abstract

Section: Is There Camp-dependent Signaling In Plants?mentioning

confidence: 99%

Adenyl cyclases and cAMP in plant signaling - past and present

2010

Self Cite

View full text Add to dashboard Cite

show abstract

“…The characterization of knockout mutants of CNGC‐encoding genes provided the strongest genetic evidence for cAMP function in cellular Ca 2+ increase, which was also demonstrated to be involved in plant defence responses (Clough et al ., ; Balagué et al ., ). In response to avirulent pathogens, cAMP elevation functioned upstream of reactive oxygen species (ROS) and nitric oxide (NO) production, through the direct modulation of CNGCs (Ma et al ., , ).…”

Section: Introductionmentioning

confidence: 99%

Genetic buffering of cyclic AMP in Arabidopsis thaliana compromises the plant immune response triggered by an avirulent strain of Pseudomonas syringae pv. tomato

et al. 2019

View full text Add to dashboard Cite

Cyclic AMP plays important roles in different physiological processes, including plant defence responses. However, as little information is known on plant enzymes responsible for cAMP production/degradation, studies of cAMP functions have relied, to date, on non-specific pharmacological approaches. We therefore developed a more reliable approach, producing transgenic Arabidopsis thaliana lines overexpressing the 'cAMP-sponge' (cAS), a genetic tool that specifically buffers cAMP levels. In response to an avirulent strain of Pseudomonas syringae pv. tomato (PstAvrB), cAS plants showed a higher bacterial growth and a reduced hypersensitive cell death in comparison with wild-type (WT) plants. The low cAMP availability after pathogen infection delayed cytosolic calcium elevation, as well as hydrogen peroxide increase and induction of redox systems. The proteomic analysis, performed 24 h post-infection, indicated that a core of 49 proteins was modulated in both genotypes, while 16 and 42 proteins were uniquely modulated in WT and cAS lines, respectively. The involvement of these proteins in the impairment of defence response in cAS plants is discussed in this paper. Moreover, in silico analysis revealed that the promoter regions of the genes coding for proteins uniquely accumulating in WT plants shared the CGCG motif, a target of the calcium-calmodulinbinding transcription factor AtSR1 (Arabidopsis thaliana signal responsive1). Therefore, following pathogen perception, the low free cAMP content, altering timing and levels of defence signals, and likely acting in part through the mis-regulation of AtSR1 activity, affected the speed and strength of the immune response. Role of cAMP in plant immune response 597Generation of Arabidopsis cAS-mCherry plants expressing the NES-YC3.6 probeThe Arabidopsis Col-0 cAS-transgenic lines were crossed with the Col-0 pUBQ10-NES-YC3.6 line reported in Krebs et al. (2012). Seeds from cross-pollinated flowers were surface sterilized by vapour-

show abstract

“…Of the 56 coding sequences identified at present as cation-conducting channels in the Arabidopsis thaliana genome, 20 are members of the CNGC family (Ward et al, 2009 ). CNGCs have been functionally characterized by expression in heterologous systems, or by analysis of cation-related phenotypes of mutant plants (typically A. thaliana ) that have specific CNGC genes silenced (Talke et al, 2003 ; Ma et al, 2010 ). All relevant experimental evidence indicates that plant CNGCs are specifically localized to the plasma membrane, although CNGC20 may be targeted to the chloroplast envelope according to a putative annotation in UniProt 1 (accessed August 28, 2011).…”

mentioning

confidence: 99%

“…All relevant experimental evidence indicates that plant CNGCs are specifically localized to the plasma membrane, although CNGC20 may be targeted to the chloroplast envelope according to a putative annotation in UniProt 1 (accessed August 28, 2011). Functional analyses of members of this channel family have associated many of them with inward K + and Ca 2+ currents, and at least in several cases, Na + conductance (Ma et al, 2010 ). It may be relevant to their function in plants that in one case, K + conductance by a CNGC was restricted in the presence of millimolar concentrations of external Ca 2+ (Leng et al, 2002 ).…”

mentioning

confidence: 99%

Evolutionary and Structural Perspectives of Plant Cyclic Nucleotide-Gated Cation Channels

Zelman¹,

Dawe²,

Gehring³

et al. 2012

Front. Plant Sci.

Self Cite

123

104

View full text Add to dashboard Cite

Ligand-gated cation channels are a frequent component of signaling cascades in eukaryotes. Eukaryotes contain numerous diverse gene families encoding ion channels, some of which are shared and some of which are unique to particular kingdoms. Among the many different types are cyclic nucleotide-gated channels (CNGCs). CNGCs are cation channels with varying degrees of ion conduction selectivity. They are implicated in numerous signaling pathways and permit diffusion of divalent and monovalent cations, including Ca2+ and K+. CNGCs are present in both plant and animal cells, typically in the plasma membrane; recent studies have also documented their presence in prokaryotes. All eukaryote CNGC polypeptides have a cyclic nucleotide-binding domain and a calmodulin binding domain as well as a six transmembrane/one pore tertiary structure. This review summarizes existing knowledge about the functional domains present in these cation-conducting channels, and considers the evidence indicating that plant and animal CNGCs evolved separately. Additionally, an amino acid motif that is only found in the phosphate binding cassette and hinge regions of plant CNGCs, and is present in all experimentally confirmed CNGCs but no other channels was identified. This CNGC-specific amino acid motif provides an additional diagnostic tool to identify plant CNGCs, and can increase confidence in the annotation of open reading frames in newly sequenced genomes as putative CNGCs. Conversely, the absence of the motif in some plant sequences currently identified as probable CNGCs may suggest that they are misannotated or protein fragments.

show abstract

The Function of Cyclic Nucleotide-Gated Channels in Biotic Stress

Cited by 15 publications

References 77 publications

Adenyl cyclases and cAMP in plant signaling - past and present

Adenyl cyclases and cAMP in plant signaling - past and present

Genetic buffering of cyclic AMP in Arabidopsis thaliana compromises the plant immune response triggered by an avirulent strain of Pseudomonas syringae pv. tomato

Evolutionary and Structural Perspectives of Plant Cyclic Nucleotide-Gated Cation Channels

Contact Info

Product

Resources

About