An azole-resistant isolate of Malassezia pachydermatis

Nijima, Misako; Kano, Rui; Nagata, Masahiko; Hasegawa, Atsuhiko; Kamata, Hiroshi

doi:10.1016/j.vetmic.2010.10.010

Cited by 58 publications

(56 citation statements)

References 9 publications

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“…Control cultures were achieved with medium alone, and with medium supplemen- ted with 50% of sweet almond oil, respectively. Furthermore, the yeasts were tested against ketoconazole by E-test (AB Biodisk, Solna, Sweden), as described by Nijima et al, (2010) [16]. Cultures were incubated at 30 °C for 24-72 h, until a full development of the yeasts in control wells was noticeable.…”

Section: Introductionmentioning

confidence: 99%

Antimycotic Activity of Some Aromatic Plants Essential Oils Against Canine Isolates of Malassezia pachydermatis: An in vitro Assay

Pistelli¹,

Mancianti²,

Bertoli³

et al. 2012

TOMYCJ

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Section: Introductionmentioning

confidence: 99%

Antimycotic Activity of Some Aromatic Plants Essential Oils Against Canine Isolates of Malassezia pachydermatis: An in vitro Assay

Pistelli¹,

Mancianti²,

Bertoli³

et al. 2012

TOMYCJ

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“…Previously, we reported that a clinical isolate of M. pachydermatis was resistant to itraconazole [9]. Cafarchia et al .…”

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confidence: 99%

<i>In Vitro</i> Susceptibility of <i>Malassezia pachydermatis</i> Isolates from Canine Skin with Atopic Dermatitis to Ketoconazole and Itraconazole in East Asia

et al. 2014

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Topical or oral azole antifungals are commonly used in canine atopic dermatitis (AD), as the lipophilic yeast Malassezia pachydermatis exacerbates canine AD. To examine whether canine AD lesions harbor azole-resistant M. pachydermatis isolates in East Asia, we investigated the in vitro susceptibility of M. pachydermatis isolates to ketoconazole (KTZ) and itraconazole (ITZ) obtained from AD lesions of canines in Japan, Korea and Taiwan. The minimum inhibitory concentrations (MICs) of KTZ and ITZ were measured by the E-test using Sabouraud dextrose agar with 0.5% Tween 40. The MICs of KTZ and ITZ for isolates from canines with AD were significantly higher than the MICs for isolates from healthy canines. Our findings suggested that the clinical isolates from canine AD skin lesions were less susceptible to azoles than those from normal canine skin in East Asia.

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“…A variety of alternative procedures, most of which use lipid-enriched media, have been proposed, but published studies have mainly focused on M. pachydermatis susceptibility to ketoconazole, itraconazole, and other azole derivatives (e.g., [8][9][10][11][12][13][14][15][16][17]. On the contrary, information on the in vitro susceptibility of M. pachydermatis isolates to the polyene amphotericin B, which is commonly used for treating bloodstream infections in human patients (5-7), is scarcer; the available studies were based on a single method and/or a low number of isolates (see Table 1), so the reliability of their results cannot be adequately assessed.…”

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confidence: 99%

In Vitro Amphotericin B Susceptibility of Malassezia pachydermatis Determined by the CLSI Broth Microdilution Method and Etest Using Lipid-Enriched Media

Álvarez‐Pérez

Blanco

Peláez

et al. 2014

Antimicrob Agents Chemother

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We determined the in vitro amphotericin B susceptibility of 60 Malassezia pachydermatis isolates by the CLSI broth microdilution method and the Etest using lipid-enriched media. All isolates were susceptible at MICs of <1 g/ml, confirming the high activity of amphotericin B against this yeast species. Overall, the essential agreement between the tested methods was high (80% and 96.7% after 48 h and 72 h, respectively), and all discrepancies were regarded as nonsubstantial. Malassezia pachydermatis is a lipophilic but non-lipid-dependent basidiomycetous yeast which thrives on the skin of most warm-blooded vertebrates (1-3). Although generally regarded as a commensal microorganism, M. pachydermatis can become pathogenic under the influence of diverse factors, leading to different clinical forms of dermatitis and/or otitis, mainly in small animals (1, 2, 4). Systemic infections caused by this yeast species are only rarely encountered, but some outbreaks of fungemia have been reported in intensive care nurseries (5, 6). Notably, in one such outbreak, the introduction of the yeast into a nursery was linked to its possible zoonotic transmission from colonized pet dogs to the health care workers' hands, with further spread of the microorganism in the unit through person-to-person contact (5).Antifungal susceptibility testing of Malassezia species remains a challenge, as their growth is not supported (or, in the case of M. pachydermatis, only poorly supported) on the standard lipid-free RPMI growth medium recommended for yeast testing by the CLSI and EUCAST (6-8). A variety of alternative procedures, most of which use lipid-enriched media, have been proposed, but published studies have mainly focused on M. pachydermatis susceptibility to ketoconazole, itraconazole, and other azole derivatives (e.g., 8-17). On the contrary, information on the in vitro susceptibility of M. pachydermatis isolates to the polyene amphotericin B, which is commonly used for treating bloodstream infections in human patients (5-7), is scarcer; the available studies were based on a single method and/or a low number of isolates (see Table 1), so the reliability of their results cannot be adequately assessed.The aim of this study was to expand the database of MIC values to amphotericin B for M. pachydermatis isolates, as determined by two different procedures using lipid-enriched media, (i) a modified broth microdilution (BMD) method based on CLSI guidelines (18) and (ii) the Etest, which is a commercial agar-based gradient technique that is widely used for antifungal susceptibility testing of yeasts.Isolates. A total of 60 Malassezia pachydermatis isolates obtained from clinical cases of canine otitis and primarily recovered in Sabouraud dextrose agar (SDA) containing 0.005% chloramphenicol (bioMérieux, Marcy l'Etoile, France) were included in this study. They were identified as M. pachydermatis on the basis of their macroscopic and microscopic morphologies and their ability to grow on media without lipid supplementation, such as SDA, at 32°C (19)...

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An azole-resistant isolate of Malassezia pachydermatis

Cited by 58 publications

References 9 publications

Antimycotic Activity of Some Aromatic Plants Essential Oils Against Canine Isolates of Malassezia pachydermatis: An in vitro Assay

Antimycotic Activity of Some Aromatic Plants Essential Oils Against Canine Isolates of Malassezia pachydermatis: An in vitro Assay

<i>In Vitro</i> Susceptibility of <i>Malassezia pachydermatis</i> Isolates from Canine Skin with Atopic Dermatitis to Ketoconazole and Itraconazole in East Asia

In Vitro Amphotericin B Susceptibility of Malassezia pachydermatis Determined by the CLSI Broth Microdilution Method and Etest Using Lipid-Enriched Media

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