An automated system for analyzing agarose and polyacrylamide gel images

Abstract

Agarose or polyacrylamide gel electrophoresis is a common technique used to separate nucleic acids (DNA and RNA) according to their molecular weight, which generates images that can be analyzed automatically by a system. The separated DNA fragments of different molecular weights give a series of bands with positions in the gel image corresponding to their molecular weights. Automated image analysis of the gels removes much of the subjectivity of manual interpretation of band positions and sizes. In this paper, an automated system was designed for analyzing DNA bands using image and signal processing techniques. The proposed algorithm consists of four main steps: preprocessing to enhance the image, detecting lanes and bands, identifying band lengths and clustering; in which the similarities of biological samples based on shared bands can be identified. The proposed technique eliminates defects due to noise and double bands in images.

A new DNA-based model for finite field arithmetic

“…This offers the advantages of a uniform loading of samples, time saving and avoidance of the error prone process of handling and loading larger number of samples by hand. Another area where improvements can be incorporated is in reading and interpreting of the results through image analysis by simple software, for example (Intarapanich et al, 2015;Abeykoon et al, 2015). This software can be of great help, as it can quickly and accurately read and translate the image results in a user-friendly format.…”

Analisis Hasil Elektroforesis DNA dengan Image Processing Menggunakan Metode Gaussian Filter

“…Namun, pada saat nilai confidence kecil dengan nilai 40 dan nilai marker DNA 1000 Bp menghasilkan error 17.76% sehingga masih memiliki error yang cukup besar. Studi yang dilakukan oleh [13] menunjukkan bahwa sebuah sistem yang di desain untuk menganalisis band DNA menggunakan teknik image dan signal processing telah cukup bagus. Metode yang di desain ini melalui 4 step yaitu preprocessing, pendeteksian garis dan band, identifikasi panjang band, dan klasterisasi.…”

RAPD based Genetic Diversity of Endangered Himalayan Gray Langur (Semnopithecus ajax) Populations of Pakistan

“…Scoring of bands: Beside visual observations, the Software (GelAnalyzer-2010a) was used to record presence or absence of bands (Arruda et al, 2012;Abeykoon et al, 2015). Binary matrix of each genotype was recognized as presence "1" and absence "0" of bands.…”