An Automated Method for the Determination of Estrogens in Pregnancy

Abstract

Ittrich’s method for the determination of total estrogens in urine (1) was adapted for automation on the AutoAnalyzer, using a continuous digester module for the combined operation of phase exchange and color development. The automated method gave good recovery and reproducibility, and correlated well with the manual method. It increased the capacity of this laboratory for estrogen determinations, improved the turnover rate, and reduced the labor involved to a minimum.