An Automated Fluorometric Method for Determination of Lactic Dehydrogenase in Serum

Brooks, Lewis; Olken, Harry G.

doi:10.1093/clinchem/11.8.748

Cited by 64 publications

(3 citation statements)

References 16 publications

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“…Specific key findings of these studies are summarized in Table 1. Air pollutant particle effects in cellular viability and toxicity are conventionally assessed by measuring NADPH-dependent oxidoreductases and lactate dehydrogenase release respectively (Brooks & Olken, 1965;Shelton & Schneider, 1952). We (via unpublished results) and others have shown that exposure to PM led to reduced viability and cytotoxicity in olfactory and primary human nasal epithelial cells (Hong et al, 2016).…”

Section: Air Pollution Effects On Olfactory Epithelial Cells In Vitromentioning

confidence: 93%

Olfactory cell cultures to investigate health effects of air pollution exposure: Implications for neurodegeneration

Lampinen

Rantanen

Odendaal

et al. 2020

Neurochemistry International

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Section: Air Pollution Effects On Olfactory Epithelial Cells In Vitromentioning

confidence: 93%

Olfactory cell cultures to investigate health effects of air pollution exposure: Implications for neurodegeneration

Lampinen

Rantanen

Odendaal

et al. 2020

Neurochemistry International

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“…[Argininelvasopressin and ATPase activity were measured as described previously . Lactate dehydrogenase (EC 1.1.1.27) activity was assayed by the method of Brooks & Olken (1965), and that of glutamate dehydrogenase (EC 1.4.1.2) as described by Schmidt (1970). Protein was determined by the method of Lowry et al (1951), with bovine serum albumin as standard.…”

Section: Assayfor Subcellular Markersmentioning

confidence: 99%

Complex phosphorylation activity in neurosecretosomal membranes isolated from ox neurohypophyses

Treiman¹,

Worm-Petersen²,

Thorn³

1980

Biochemical Journal

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Homogenates of neural lobes of bovine pituitary glands were fractionated on Ficoll gradients to yield neurosecretosomes (nerve endings). The neurosecretosomes were lysed in a hypo-osmotic buffer and the membranes were separated from the soluble components by centrifugation. On incubation with [gamma-32P]ATP this membrane preparation showed an endogenous phosphorylation activity, which was studied by means of gel electrophoresis in the presence of sodium dodecyl sulphate, and subsequent autoradiography. The major part of the [32P]Pi detected on the gel was shown to be incorporated into three protein bands, termed A, B and C, with minimal mol.wts. of 83 000, 59 000 and 47 000 respectively. The phosphorylation of these three proteins was studied under a variety of experimental conditions. The patterns obtained were partly similar. However, important individual differences were noted, particularly with respect to the effects of cyclic AMP, Mg2+ and Ca2+. On the basis of these differences, it is suggested that in this system the phosphorylation activity is heterogenous, bands A, B and C each reflecting the presence of a different site of phosphate turnover. The relationship of bands A, B and C to several of the previously described phosphoproteins in the brain is discussed.

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“…A second general principle for the assay of a variety of enzymes is to measure the change in fluorescence produced by the reduction or oxidation of pyridine nucleotides in the coupled reaction. This has been used for dihydrofolic reductase (70), transaminase (339), transhydrogenase (238), and lactic and other dehydrogenases (5,69,106, 156, 185). Spectrofluorometric methods have been described for the pyridine nucleotides as such (182,323,397) and for flavine adenine dinucleotide (114).…”

Section: Organic and Biologicalmentioning

confidence: 99%

Fluorometric Analysis

White¹,

Weissler²

1966

Anal. Chem.

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This review coversthe two-year period from approximately December 1963 {465) to December 1965. Bozhevol'nov {56) has published a review on the luminescence of inorganic substances with 172 references and on fluorescence analysis for the determination of inorganic trace impurities in high purity substances {57).Shcherbov {388) has published a 259page book with 681 references on the use of fluorometry in the chemical analysis of mineral raw materials. Topics included are: general considerations, basic laws, apparatus, reagents

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An Automated Fluorometric Method for Determination of Lactic Dehydrogenase in Serum

Cited by 64 publications

References 16 publications

Olfactory cell cultures to investigate health effects of air pollution exposure: Implications for neurodegeneration

Olfactory cell cultures to investigate health effects of air pollution exposure: Implications for neurodegeneration

Complex phosphorylation activity in neurosecretosomal membranes isolated from ox neurohypophyses

Fluorometric Analysis

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