An automated biological assay to determine levels of the trypanocidal drug melarsoprol in biological fluids

Mismatch repair in Trypanosoma brucei: Heterologous expression of MSH2 from Trypanosoma cruzi provides new insights into the response to oxidative damage

“…After 48 h growth, 20 μl of Alamar blue (12.5 mg ml − 1 ) was added to each well. Plates were kept at 37°C in the dark and 24 h later the fluorescence emitted by Alamar blue reduction by live cells in each well was determined fluorometrically (PerkinElmer LS55) at 590 nm (Räz et al, 1997;Onyango et al, 2000). The fluorescence emitted was compared to that of cells cultured in the absence of MNNG and expressed as percentage survival (taking the cells without drug as 100%).…”

An antimicrobial peptide with trypanocidal activity characterized from Glossina morsitans morsitans

“…3B). For these experiments, parasite inhibition was evaluated using the chromogenic/fluorogenic substrate alamarBlue which measures cell viability and proliferation based on detection of metabolic activity (Haines et al, 2003;Onyango et al, 2000;Raz et al, 1997). The assay incorporates an oxidation-reduction indicator that changes color in response to chemical reduction by metabolically active cells from its oxidized form (blue, non-fluorescent) to reduced form (red, fluorescent).…”

Natural products as potential antiparasitic drugs

“…Sensitive molecular techniques such RT-PCR -while providing little advantage when quantifying an acute infection -are useful for detection of latent infections after parasitological cure. [32], the MTT assay (described in section 3.2) or a fluorochrome such as Alamar blue [33]. Plotting % growth inhibition {= [1 -δ sample -δ max kill ) / (δ max growth -δ max kill )] x 100} against drug concentration, dose-response curves can be generated and EC 50 and EC 90 values calculated for better comparison.…”