An attempt to classify the plasma proteins of the rainbow trout (Salmo gairdnerii Richardson) using disc electrophoresis, gel filtration and salt solubility fractionation

The plasma proteins of Labeo umbratus (Smith) and Labeo capensis (Smith)

“…Tt is therefore at present not possible to remark on the nature of the albumins and globulins of the species studied. This view is in agreement with that of Mulcahy (1970) and that of Perrier et al (1973). The latter workers suggest a completely separate nomenclature for trout plasma proteins after initial biochemical investigation.…”

Assessment of serum protein fractions in rainbow trout using automated electrophoresis and densitometry

“…Protein electrophoresis has been applied to serum samples from fish since 1949, using many different methods and resulting in the resolution of different fractions 1–9 . Among fish, rainbow trout ( Oncorhynchus mykiss , Walbaum) are one of the most‐studied fish.…”

“…Deutsch and McShan 1 obtained 8 protein fractions as a result of “free electrophoresis” in serum from rainbow trout ( O. mykiss ). Disk gel electrophoresis resulted in 13 main bands in rainbow trout plasma, 3,6,11 although additional bands were also reported to occur occasionally 3 . Agarose gel electrophoresis of rainbow trout plasma resulted in 11 protein fractions (3 in the albumin zone, 4 in the α‐globulin zone, 3 in the β‐globulin zone, and 1 in the γ‐globulin zone).…”

“…Fibrinogen was identified in the 12th band (of 13) by Perrier et al 3 by comparison of plasma and serum on a disk electrophoretic tracing. Booth et al 11 found that the same band 12 disappeared in chlorine‐exposed rainbow trout and correlated this finding with severe hemorrhage in the exposed fish.…”

The Plasma Proteins of Some Freshwater Fish in Their Natural Environment

“…Species specific electrophoretograms have been found with wide individual variations as well as seasonal fluctuations evident in the concentration of each protein fraction (Uthe, Roberts, Clarke & Tsuyuki, ]966;Fourie&Hattingh, 1976;Van Vuren&Hattingh, 1976). Mulcahy (1970), Perrier, Del e r oi x , Perrier &Gras (1973) and Hattingh (1974a) have pointedout that the application of the observations pertaining to human plasma proteins cannot be directly applied to fish plasma proteins and are therefore of dubious value.…”