An ATP-based method for monitoring the microbiological drinking water quality in a distribution network

Delahaye, E.; Welté, B.; Lévi, Yves; Leblon, Gérard; Montiel, A.

doi:10.1016/s0043-1354(03)00288-4

Cited by 96 publications

(59 citation statements)

References 11 publications

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“…Because the ATP method has mainly been applied in monitoring the microbiological quality of the drinking water [12,14,33,34], its recommended protocol [26] should be optimized when used in detecting ATP in sludge. In this study, the effects of sludge concentration and incubation time were investigated.…”

Section: Optimize Atp Assay For Fast Detection Of the Quantity Of Viamentioning

confidence: 99%

“…On the contrary, the adenosine triphosphate (ATP) bioluminescence assay, based on the direct proportionality of ATP to viable biomass, can provide a more accurate estimate of the total active biomass in a sample than the CFU method [7,11,12]. The ATP assay, mainly used in monitoring microbiological drinking water quality [12][13][14], allows an estimation of bacterial populations within minutes and can be done on-site, which is particularly suitable for assessing the reduction of viable bacteria during activated sludge ozonation, but the use of the ATP assay for this application has not yet been reported. As for the change in bacterial community composition, a loss of bacterial diversity was found with the increase of ozone dosage by polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE) [5].…”

Section: Introductionmentioning

confidence: 99%

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Detection of Viable Bacteria during Sludge Ozonation by the Combination of ATP Assay with PMA-Miseq Sequencing

Tian

Yang

et al. 2017

Water

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Using sludge obtained from municipal sewage treatment plants, the response of viable bacterial populations during the sludge ozonation process was investigated by a combination of adenosine triphosphate (ATP) assay and propidium monoazide (PMA)-Miseq sequencing. The ATP assay was first optimized for application on sludge samples by adjusting the sludge solid contents and reaction time. PMA-modified polymerase chain reaction (PCR) was also optimized by choosing the suitable final PMA concentration. The quantity and composition of viable bacterial populations during sludge ozonation were further elucidated using the optimized ATP and PMA-modified PCR methods. The results indicated that after the sludge was exposed to ozone (O3) at 135 mg·O3/g total suspended solids (TSS), the viable biomass displayed a substantial decrease, with a reduction rate reaching 70.89%. The composition of viable bacterial communities showed a faster succession, showing that an ozone dosage of 114 mg·O3/g TSS is enough to significantly change the viable bacterial population structure. Floc-forming genera, such as Zoogloea, Ferruginibacter, Thauera and Turneriella, are sensitive to ozonation, while the relative abundances of some functional bacterial genera, including SM1A02, Nitrospira and Candidatus Accumulibacter, remained constant or increased in the viable bacterial population during sludge ozonation, indicating that they are more resistant to ozonation.

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Section: Optimize Atp Assay For Fast Detection Of the Quantity Of Viamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Detection of Viable Bacteria during Sludge Ozonation by the Combination of ATP Assay with PMA-Miseq Sequencing

Tian

Yang

et al. 2017

Water

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“…Water disinfection creates stressing conditions in bacteria and induces the VBNC state. The process also takes place in cells that make the biofilm, which negatively affects water quality and can also counteract the effects of disinfectants [41]. Importantly, cells in the VBNC state can occur in the biofilm that does not undergo disinfection processes.…”

Section: Microbiological Analyses Of Drinking Watermentioning

confidence: 99%

Using ATP tests for assessment of hygiene risks

Pistelok

Pohl

Stuczyński

et al. 2016

Ecological Chemistry and Engineering S

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Adenosine-5'-triphosphate (ATP) is a high-energy molecule playing a role of universal energy carrier used in cell metabolism. Since ATP is present in both prokaryotic and eukaryotic cells, this coenzyme can be used to determine a hygiene state. During last decade there has been a significant growth of practical applications of ATP measurements. ATP tests are commonly used for an assessment of hygienic conditions in food industry, residential buildings and hotels. In comparison with traditional and time-consuming laborious microbial analysis the measurement of ATP provides a quick indicative on-site data, characterizing microbial and organic contamination to support decision-making with regards to adequate corrective and preventive actions. This paper demonstrates applications of ATP in practice and, particularly, in water treatment technology and wastewater processing. The review highlights opportunities for using the test for the optimization of water treatment facilities and pipe networks functioning in order to assess wastewater toxicity and wastewater treatment process efficiency. Other areas of application are also addressed. The review of literature indicates that ATP used in different areas of industry can be considered as a diagnostic method -complement the classical methods -useful for identification of hygiene risks as well as a tool for a real-time management of health and environmental risks.

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“…En effet, la conception de réseaux de distribution d'eau potable et d'eau chaude domestique ainsi que des systèmes aéroréfrigérants ont créé de nouveaux écosystèmes dans lesquels les microorganismes ont pu se développer. Outre les contaminations liées à des accidents d'exploitation (au niveau des filières de traitements ou des infrastructures de stockage et de distribution), les microorganismes peuvent se multiplier dans le système de distribution (BEAUDEAU et al, 2007;DELAHAyE et al, 2003;LECHEvALLIER et al, 1987;LECHEvALLIER et al, 1996). Cette contamination de l'eau potable peut engendrer non seulement une dégradation de la qualité organoleptique, mais aussi des cas de maladie d'origine hydrique (BERRy et al, 2006).…”

Section: Les Maladies D'origine Hydrique Et La Potabilité De L'eauunclassified

Le réseau de distribution d’eau potable : un écosystème complexe lié à des enjeux de santé publique

Poitelon

Joyeux

Welté

et al. 2012

rseau

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L’émergence de pathogènes dans l’eau destinée à la consommation humaine représente une préoccupation majeure en matière de santé publique pour les industriels et les pouvoirs publics concernés. Parmi ces pathogènes, certains sont d’origine fécale (Cryptosporidium, Campylobacter ou bien les rotavirus), alors que d’autres vivent dans l’environnent naturel (Legionella, Pseudomonas, Aeromonas ou bien les mycobactéries). Dans l’optique de mettre en place une analyse des risques liés à la présence de ces pathogènes, il est important d’accroître nos connaissances sur l’écologie de ces microorganismes et de développer des outils d’analyse afin de réaliser une meilleure surveillance sanitaire. Par conséquent, l’écologie microbienne du réseau de distribution d’eau potable doit être étudiée en détail, particulièrement vis-à-vis des propriétés physiologiques et la diversité des espèces microbiennes présentes, afin de mieux comprendre les interactions entre les espèces communément rencontrées et celles pathogènes.The emergence of pathogens in water intended for human consumption is a major concern in terms of the public health industry and the public authorities concerned. Among these pathogens, some are of faecal origin (Cryptosporidium, Campylobacter, or rotavirus), while others live in the natural environment (Legionella, Pseudomonas, Aeromonas or mycobacteria). In order to establish a risk analysis related to the presence of these pathogens, it is important to increase our knowledge on the ecology of these microorganisms and to develop analytical tools to achieve better health monitoring. Therefore, the microbial ecology of drinking water distribution networks must be studied in detail, especially with respect to the properties and physiological diversity of the microbial species present, in order to better understand the interactions between species commonly encountered and those that are pathogens

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An ATP-based method for monitoring the microbiological drinking water quality in a distribution network

Cited by 96 publications

References 11 publications

Detection of Viable Bacteria during Sludge Ozonation by the Combination of ATP Assay with PMA-Miseq Sequencing

Detection of Viable Bacteria during Sludge Ozonation by the Combination of ATP Assay with PMA-Miseq Sequencing

Using ATP tests for assessment of hygiene risks

Le réseau de distribution d’eau potable : un écosystème complexe lié à des enjeux de santé publique

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