An assessment of inositol fermentation by Klebsiellae and its implication in screening media.

Barr, J. G.; Mahood, R J

doi:10.1136/jcp.29.9.827

Cited by 6 publications

(11 citation statements)

References 6 publications

(4 reference statements)

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“…§Of a total of 11 isolates 8 were from patients in closely adjacent wards and represented repeated isolations from three different patients. (Barr and Mahood, 1976). Our results showed the important effect of inoculum size on the results obtained in the biochemical tests described on screening media.…”

Section: Effect Of Inoculum Size On Response Produced On Specific Scrmentioning

confidence: 51%

“…Among other Klebsiella spp only Kpneumoniae could otherwise be differentiated, although stock cultures of K ozaenae could also be presumptively identified on the basis of slow inositol fermentation (Barr and Mahood, 1976). One novel biotype among the K aerogenes/edwardsii/atlantae group was characterised by the absence of aesculin hydrolysis.…”

Section: Evaluation Of Screening Scheme In Routine Usementioning

confidence: 99%

“…The value of aesculin hydrolysis (Wasilauskas, 1971; Barr and Mahood, 1977) and inositol fermentation (Donovan, 1966;Barr and Mahood, 1976) in differentiating the Klebsiella-Enterobacter-Serratia group from other Enterobacteriaceae has already been established, and Proteus spp and Providencia spp are differentiated from other Enterobacteriaceae and from one another on the basis of phenylalanine deaminase and urease activity. Other tests, including tryptophanase activity, growth in presence of potassium cyanide, citrate…”

mentioning

confidence: 99%

“…Thus the degree of aesculin hydrolysis and inositol fermentation may indicate species or biotype differences among Klebsielleae (Barr and Mahood, 1976).…”

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confidence: 99%

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Factors affecting the value of a simple biochemical scheme for identifying Enterobacteriaceae: the reproducible recognition of biotypes.

Barr¹,

Mahood²,

Curry³

1977

J Clin Pathol

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SUMMARY A biochemical screening scheme on agar media for differentiating Enterobacteriaceae in a hospital laboratory is evaluated. Careful observation of test reactions within the scheme permitted the recognition of 78 biotypic reaction patterns which could contribute to epidemiological surveillance. The limitations of the technique are described and discussed and methods of importance in ensuring the reproducibility of reaction patterns emphasised.

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Section: Effect Of Inoculum Size On Response Produced On Specific Scrmentioning

confidence: 51%

Section: Evaluation Of Screening Scheme In Routine Usementioning

confidence: 99%

mentioning

confidence: 99%

“…Thus the degree of aesculin hydrolysis and inositol fermentation may indicate species or biotype differences among Klebsielleae (Barr and Mahood, 1976).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Factors affecting the value of a simple biochemical scheme for identifying Enterobacteriaceae: the reproducible recognition of biotypes.

Barr¹,

Mahood²,

Curry³

1977

J Clin Pathol

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“…The population was very homogeneous, and few biotypes could be identified. Differences did occur in inositol fermentation (cf, Barr and Mahood, 1976), indole production, and malonate utilisation, which could usefully complement strain discrimination on the basis of differential substrate metabolism.…”

Section: Comparison Of Biochemical Typing Methodsmentioning

confidence: 98%

Biotypes of Klebsiella pneumoniae (sensu lato) and Enterobacter aerogenes characterised by differential substrate metabolism: application of the technique.

Barr¹,

Hogg²

1979

J Clin Pathol

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SUMMARY A biochemical typing method is described for Klebsiella pneumoniae (sensu lato) and Enterobacter aerogenes. The technique depends on differences in metabolism of five carbon substrates-glycerol, inositol, lactose, glucose, and xylose-at two concentrations. Reproducibility is satisfactory and is monitored by the incorporation of control klebsiellae of known biotype.The method has been used for 12 months in the surveillance of urinary tract colonisation in this hospital. Gut carriage of klebsiellae, implicated by several workers as a source of infection, was common among staff and new admissions. Many biotypes were represented which were sensitive to most antibiotics except ampicillin.Klebsiellae, all multiply resistant, were isolated most frequently from urine specimens in two orthopaedic wards. In a longitudinal study in these wards, a sequential dominance in urinary tract colonisation by two klebsiella biotypes was shown, which suggested the presence of cross infection or an environmental reservoir. Confirmatory evidence was obtained from capsular serotypes and R-factor studies.Klebsiellae are one of the major causes of hospital acquired infection. This has led to the development of many methods which aimed to detect identical strains of the same species and thus provide a reliable method for epidemiological studies.Serological (Casewell, 1972;Riser et al., 1976; Palfreyman,1978) Received for publication 7 March 1979 Biochemical typing, however, if feasible, would be quick and inexpensive and could overcome the requirements for special reagents and special expertise often needed by other methods. Barr (1978) identified a number of biochemical test substrates whose differential metabolism provided good discrimination between isolates of klebsiella. In our experience, differential metabolism of these substrates also allowed discrimination between isolates of Enterobacter aerogenes. The various biotypes of Klebsiella pneumoniae (sensu lato) and Enterobacter aerogenes distinguished by the differential metabolism of five selected substrates are described here.This technique is applied to surveillance of biochemical types of klebsiella associated with colonization of the urinary tract. The results suggest that the preponderance of certain biotypes in some wards could be attributed to cross infection or to a common source. Material and methods ORGANISMS: ISOLATION AND IDENTIFICATIONA total of 445 clinical isolates of klebsiellae were examined over a 12-month period. These included 935 on 12 May 2018 by guest. Protected by copyright.

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Bacteriocin-producing lactic acid bacteria isolated from mangrove forests in southern Thailand as potential bio-control agents in food: Isolation, screening and optimization

2014

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An assessment of inositol fermentation by Klebsiellae and its implication in screening media.

Cited by 6 publications

References 6 publications

Factors affecting the value of a simple biochemical scheme for identifying Enterobacteriaceae: the reproducible recognition of biotypes.

Factors affecting the value of a simple biochemical scheme for identifying Enterobacteriaceae: the reproducible recognition of biotypes.

Biotypes of Klebsiella pneumoniae (sensu lato) and Enterobacter aerogenes characterised by differential substrate metabolism: application of the technique.

Bacteriocin-producing lactic acid bacteria isolated from mangrove forests in southern Thailand as potential bio-control agents in food: Isolation, screening and optimization

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