An assessment of adaptive and antagonistic properties of Trichoderma sp. strains in vegetable waste composts

Abstract

The experiment consisted in monitoring the count of moulds and three selected Trichoderma sp. isolates (T1 - Trichoderma atroviride, T2 - Trichoderma harzianum, T3 - Trichoderma harzianum) in vegetable (onion and tomato) waste composted with additives (straw, pig manure). Additionally, the aim of the study was to determine the type of interaction occurring between autochthonous fungi isolated from composts after the end of the thermophilic phase and Trichoderma sp. strains applied in the experiment. Number of microorganisms was determined by the plate method, next the identification was confirmed. The rating scale developed by Mańka was used to determine the type of interactions occurring between microorganisms. The greatest count of moulds in onion waste composts was noted in the object which had simultaneously been inoculated with two strains T1 - T. atroviride and T3 - T. harzianum. The greatest count of moulds was noted in the tomato waste composts inoculated with T2 - T. harzianum strain. Microscope identification revealed that Penicillum sp., Rhizopus sp., Alternaria sp. and Mucor sp. strains were predominant in onion waste composts. In tomato waste composts Penicillium was the predominant genus, followed by Rhizopus. The test of antagonism revealed the inhibitory effect of Trichoderma isolates on most autochthonous strains of moulds. Tomato waste composts proved to be better substrates for the growth and development of Trichoderma sp. isolates. The results of the study show that vegetable waste can be used in agriculture as carriers of antagonistic microorganisms.

Energy and economic potential of maize straw used for biofuels production

“…The dry matter content was made according to Polish standard norm PN-75C-04616/01 (drying by 24 h in 105 o C), whereas organic dry matter according to PN-Z-15011-3 norm (samples combustion in 525 o C for 3 hours) [20][21].…”

The Use of Lignin as a Microbial Carrier in the Co-Digestion of Cheese and Wafer Waste

“…The substrates and the digested materials were analyzed using the following methods and devices: the carbon content-combustion at 900 • C, CO 2 -OI Analytical analyzer; nitrogen-titration, Kjeldahl method with 0.1 n HCl; ammonium nitrogen-distillation and titration with 0.1 n HCl; phosphorus-the mineralization of phosphorus compounds with nitric acid (microwave furnace, Milestone), then by spectrophotometric analysis (Varian Cary 50); chemical oxygen demand (CODr)-titration, dichromate method (potassium dichromate, concentrated sulphuric acid, silver sulphate as catalyst); light metal ions-inductively coupled plasma optical emission spectrometry (ICP-OES, Sysmex, Hoeilaart, Belgium), JY 2000 2 ICP-OES spectrometer (ISA Jobin Yvon, Edison, NJ, USA) [15,36].…”