“…Platelet-rich plasma samples were pre-incubated for 15 min at room temperature with 5 µL of one of the following monoclonal antibodies: P2Y12 Platelet ADP Receptor antibody (Sigma-Aldrich, Saint Louis, MO, USA), CD62P/P-Selectin Antibody APC conjugate (Thermo Fisher Scientific ® , Waltham, MA, USA), Integrin alpha-2b/CD41 Antibody FITC conjugate (Thermo Fisher Scientific ® , Waltham, MA, USA), and Integrin beta-3/CD61 Antibody APC conjugate (Thermo Fisher Scientific, Waltham, MA, USA), which have specificity for the platelet antigens P2Y12, P-Selectin (CD62P), GpIIb (CD41), and GpIIIa (CD61), respectively. We characterized the basal state of platelet activation, measured by the mean fluorescence intensity (MFI) of the plasma, which means the higher the fluorescence, the greater the labeling of antibodies against the plasma membrane [ 12 ]. For each sample submitted to flow cytometry, 10,000 events were analyzed using Attune NxT Software (Thermo Fisher Scientific, Waltham, MA, USA).…”