Detection of Sepsis in Platelets Using MicroRNAs and Membrane Antigens

Corrêa, Priscilla Cristina Moura Vieira; Carneiro, Débora Monteiro; Valente, L; Diogo, Fabíola Marques; Lamarão, Letícia Martins; Maués, Jersey Heitor da Silva; Moreira-Nunes, Caroline Aquino; Burbano, Rommel Rodrı́guez

doi:10.3390/genes12121877

Cited by 3 publications

(1 citation statement)

References 39 publications

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“…Thrombocytopenia is associated with a higher mortality rate in sepsis patients [ 20 ] . Research by Vieira Corrêa PCM [ 29 ] and others has confirmed a significant correlation between low platelet counts and the severity and mortality rate of sepsis, and persistent thrombocytopenia is considered an independent risk factor for sepsis-related death. [ 19 ] The present study also shows that the platelet count of the non-survivors was lower than that of the survivors (156.99 ± 86.46 vs 267.34 ± 102.22, t = 8.539, P < .001).…”

Section: Discussionmentioning

confidence: 99%

Prognostic value of platelet combined with serum procalcitonin in patients with sepsis

Chen,

Tu,

Huang

et al. 2023

Medicine

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Sepsis, a common and life-threatening condition in critically ill patients, is a leading cause of death in intensive care units. Over the past few decades, there has been significant improvement in the understanding and management of sepsis. However, the mortality rate remains unacceptably high, posing a prominent challenge in modern medicine and a significant global disease burden. A total of 295 patients with sepsis admitted to the hospital from January 2021 to December 2022 were collected and divided into survival group and death group according to their 28-day survival status. The differences in general clinical data and laboratory indicators between the 2 groups were compared. Receiver operating characteristic curve analysis was used to evaluate the predictive value of platelet (PLT) and procalcitonin (PCT) for the prognosis of sepsis patients within 28 days. A total of 295 patients were diagnosed with sepsis, and 79 died, with a mortality rate of 26.78%. The PLT level in the death group was lower than that in the survival group; the PCT level in the death group was higher than that in the survival group. The receiver operating characteristic curve showed that the area under the curve of PCT and PLT for evaluating the prognosis of sepsis patients were 0.808 and 0.804, respectively. Kaplan–Meier survival analysis showed that the 28-day survival rate of the low PLT level group was 19.0% and that of the high PLT level group was 93.1% at the node of 214.97 × 109/L, and the difference between the 2 groups was statistically significant (χ2 = 216.538, P < .001). The 28-day survival rate of the low PCT level group was 93.4% and that of the high PCT level group was 51.7% at the node of 2.85 ng/mL, and the difference between the 2 groups was statistically significant (χ2 = 63.437, P < .001). There was a negative correlation between PCT level and PLT level (r = −0.412, P < .001). Platelet combined with serum procalcitonin detection has high predictive value for judging the 28-day prognosis of sepsis, and it can be used as an index for evaluating the patient’s condition and prognosis, and is worthy of clinical promotion and application.

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Section: Discussionmentioning

confidence: 99%

Prognostic value of platelet combined with serum procalcitonin in patients with sepsis

Chen,

Tu,

Huang

et al. 2023

Medicine

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Modes of action and diagnostic value of miRNAs in sepsis

et al. 2022

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Sepsis is a clinical syndrome defined as a dysregulated host response to infection resulting in life-threatening organ dysfunction. Sepsis is a major public health concern associated with one in five deaths worldwide. Sepsis is characterized by unbalanced inflammation and profound and sustained immunosuppression, increasing patient susceptibility to secondary infections and mortality. microRNAs (miRNAs) play a central role in the control of many biological processes, and deregulation of their expression has been linked to the development of oncological, cardiovascular, neurodegenerative and metabolic diseases. In this review, we discuss the role of miRNAs in sepsis pathophysiology. Overall, miRNAs are seen as promising biomarkers, and it has been proposed to develop miRNA-based therapies for sepsis. Yet, the picture is not so straightforward because of the versatile and dynamic features of miRNAs. Clearly, more research is needed to clarify the expression and role of miRNAs in sepsis, and to promote the use of miRNAs for sepsis management.

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MicroRNA 320a and Membrane Antigens as Tools to Evaluate the Pathophysiology of Platelets Stored in Blood Banks

Vieira

Maués

Lamarão

et al. 2022

CIMB

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Our research group, through the analysis of miRNomes in platelet concentrates (PCs) stored in blood banks, identified and validated the miR-127 and miR-320a miRNAs as biomarkers of platelet storage lesions (PSLs) in PCs. In order to validate the miRNAs 127 and 320a methodologically, as PSL biomarkers in a large number of PC bags, we also evaluated important immunological markers involved in the platelet activation/aggregation process—the CD62P receptor (P-selectin), the surface glycoproteins (GP) IIb/IIIa, and the purinergic P2Y12 receptor—via flow cytometry. The miRNAs miR-127 and miR-320a were quantified by real-time quantitative PCR (RT-qPCR). To carry out this study, 500 collection tubes were used at the upper edge of the PC bags containing platelets. Each tube was divided into seven equal parts (totaling 3500 samples) for platelet analysis from 7 different storage days, where the 1st day represents the high-quality control, and the 7th day corresponds to the low-quality control of the platelets. After analyzing all parameters during storage days, it was concluded that the relative quantification of miR-320a below 0.50 and the CD62P receptor below 27.92% are reliable indicators of the absence of storage lesions in blood banks. We believe that the values found in the expression of the CD62P receptor legitimize the use of the miR-320a and miR-127 miRNAs to build a kit capable of accurately measuring whether the stored platelets are suitable for transfusion.

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Detection of Sepsis in Platelets Using MicroRNAs and Membrane Antigens

Cited by 3 publications

References 39 publications

Prognostic value of platelet combined with serum procalcitonin in patients with sepsis

Prognostic value of platelet combined with serum procalcitonin in patients with sepsis

Modes of action and diagnostic value of miRNAs in sepsis

MicroRNA 320a and Membrane Antigens as Tools to Evaluate the Pathophysiology of Platelets Stored in Blood Banks

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