An assay of collagenase activity using enzyme-linked immunosorbent assay for mammalian collagenase

Advances in assays of matrix metalloproteinases (MMPs) and their inhibitors

“…Enzyme-linked immunosorbent assays (ELISA) have recently been developed for the quantification of MMPs [53,54]. Clark et al [55] has raised and characterized monoclonal antibodies against purified human fibroblast collagenase.…”

Proteolytic activity in infected and noninfected insect cells: Degradation of HIV-1 Pr55gag particles

“…The complex specificity of proteases is clear from the diversity of the substrates and methods that have been developed for their assays: spectrophotometry (Feder, 1968), fluorescence (Auld and Prescott, 1983;Bratovanova and Petkov, 1987;Kuromizu et al, 1985;Tisljar and Denker, 1986), HPLC (Green, 1986;Kuwada and Katayama, 1984;Nagatsu et al, 1985), ELISA (Ohlsson et al, 1986;Yoshioka et al, 1987), and methods using gel-entrapped substrates (Brown et al, 1982;Kelleher and Juliano, 1984;Nagase and Woessner, 1980) or luminogenic and chromogenic substrates (Branchini et al, 1980). When there is no information about the proteases involved, the choice of the method and substrate becomes an even larger problem.…”

Enzyme Linked Immunosorbent Assays (ELISA) for the Quantitative Determination of Human Leukocyte Collagenase and Gelatinase

“…Recently a collagenase assay has been described, which uses the highly sensitive ELISA technique for the detection of collagen degradation (16). By this assay the Substrate, but not the protease itself, is detected by ELISA.…”