An Assay for Circulating Antibodies to a Major Etiologic Virus of Human Non-A, Non-B Hepatitis

Kuo, George

doi:10.1097/00006454-199005000-00025

Cited by 644 publications

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“…Blood screening based on the cloned HCV genome and its gene products has reduced significantly the blood-borne HCV transmission [1][2][3]. However, detailed studies of HCV (for example, virus replication, pathogenicity, and natural history of HCV) have been greatly hampered by the lack of efficient in vitro culture systems of HCV infection.…”

Section: Introductionmentioning

confidence: 99%

Polypyrimidine-tract-binding Protein is a Component of the HCV RNA Replication Complex and Necessary for RNA Synthesis

et al. 2006

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The machinery for hepatitis C virus (HCV) RNA replication is still poorly characterized. The relationship between HCV RNA replication and translation is also not clear. We have previously shown that a cellular protein polypyrimidine-tract-binding protein (PTB) binds to HCV RNA at several different sites and modulates HCV translation in several ways. Here we show that PTB also participates in RNA replication. By bromouridine triphosphate (BrUTP) labeling and confocal microscopy of cells harboring an HCV replicon, we showed that the newly synthesized HCV RNA was localized to distinct structures in the cytoplasm, which also contain PTB. Membrane flotation analysis demonstrated that a fraction of cytoplasmic PTB was associated with a detergent-resistant membrane (DRM) structure consisting of lipid rafts, which also contained HCV nonstructural proteins and the human vesicle-associated membrane proteinassociated protein (hVAP-33). PTB in the DRM was resistant to protease digestion, but became sensitive after treatment with the raft-disrupting agents. PTB in the DRM consisted of multiple isoforms and the brain-specific paralog. By using small interfering RNA (siRNA) of PTB, we showed that silencing of the endogenous PTB reduced the replication of HCV RNA replicon. In a cell-free, de novo HCV RNA synthesis system, HCV RNA synthesis was inhibited by anti-PTB antibody. These studies together indicated that PTB is a part of the HCV RNA replication complex and participates in viral RNA synthesis. Thus, PTB has dual functions in HCV life cycle, including translation and RNA replication.

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Section: Introductionmentioning

confidence: 99%

Polypyrimidine-tract-binding Protein is a Component of the HCV RNA Replication Complex and Necessary for RNA Synthesis

et al. 2006

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“…It is the most common cause of sporadic non-A non-B and post-transfusion hepatitis [1]. Persistent viral infection occurs in patients infected with HCV.…”

Section: Introductionmentioning

confidence: 99%

Detection of hepatitis C virus RNA in paraffin‐embedded tissues from patients with non‐Hodgkin's lymphoma

et al. 2004

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The aim of this study is to detect the possible role of hepatitis C Virus (HCV) in lymphomagenesis. HCV‐RNA and anti‐HCV antibodies were studied in tissue and serum samples taken from patients with non‐Hodgkin's Lymphoma (NHL). The prevalence of HCV, the clinical presentation of these cases, and association with histologic subtypes were determined. RT‐PCR was used to detect the HCV‐RNA in serum and tissue samples. The anti‐HCV antibodies were tested with microparticle enzyme immunoassay. Immunohistochemistry with the ABC method was used to detect the HCV core protein in HCV‐RNA+ cases. RNA could be detected in 30 of 35 cases, and other tests were performed in these 30 samples. HCV‐RNA was detected in 11 tissue samples (11/30, 37%). HCV core protein was studied in 10 of 11 HCV‐RNA+ cases, and 1–3% nuclear staining was found in only 2 samples. Serologically, HCV‐RNA was detected in 7 of 30 samples (23.3%) and anti‐HCV antibody was detected in 3 of 30 samples (10%). Detection of HCV‐RNA in 37% of the lymphoma tissue samples suggests that HCV may have a role or is a contributing factor in the pathogenesis of lymphoma. The very low HCV core protein in lymphoma tissues may be due to the low viral load in lymphoid tissues and/or higher sensitivity of the PCR method. Detection of anti‐HCV antibody in only three cases may be associated with undetectable levels of antibodies due to the immune deficiency in cases with NHL. Am. J. Hematol. 76:252–257, 2004. © 2004 Wiley‐Liss, Inc.

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“…Copyright 1998 by the American Association for the Study of Liver Diseases H epatitis C virus (HCV) is the major etiologic agent of parenterally transmitted Non A Non B hepatitis worldwide. 1,2 In the majority of cases, the infection becomes chronic and is commonly associated with long-term complications such as cirrhosis and hepatoma. [3][4][5] It is estimated that approximately 3.5 million individuals in the United States are infected with HCV, resulting in more than 5000 deaths annually.…”

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confidence: 99%

The influence of hepatitis C virus genotypes on the outcome of liver transplantation

et al. 1998

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Background: The aim of this study was to report the influence of hepatitis C virus (HCV) genotypes and HLA matches on the outcome of liver transplantation, hepatitis recurrence, and progression to cirrhosis after transplantation. Methods: HCV genotypes were determined from pretransplantation sera and/or liver explant tissues from 202 patients with HCV-related end-stage liver disease. One hundred fifty patients with known infecting genotype for whom posttransplantation biopsy specimens were available or who had normal results of liver injury tests constituted the group analyzed. Patients were followed up for up to 4.5 years. Hepatitis activity index scores at the time of disease recurrence were used to assess disease activity. Cirrhosis was diagnosed by using histological evidence. The number of HLA matches with respect to A, B, DR, and DQ loci was deter-

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An Assay for Circulating Antibodies to a Major Etiologic Virus of Human Non-A, Non-B Hepatitis

Cited by 644 publications

References 0 publications

Polypyrimidine-tract-binding Protein is a Component of the HCV RNA Replication Complex and Necessary for RNA Synthesis

Polypyrimidine-tract-binding Protein is a Component of the HCV RNA Replication Complex and Necessary for RNA Synthesis

Detection of hepatitis C virus RNA in paraffin‐embedded tissues from patients with non‐Hodgkin's lymphoma

The influence of hepatitis C virus genotypes on the outcome of liver transplantation

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